Reducing urinary oxalate by simultaneous using Sankol herbal drop with oxalate-degrading bacteria

  • Rouhi Afkari Department of Microbiology, School of Medicine, Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
  • Mohammad Bokaeian Department of Microbiology, School of Medicine, Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
  • Soroosh Dabiri Department of Laboratory Sciences, Zahedan University of Medical Sciences, Zahedan, Iran
  • Habib Ghaznavi Department of Pharmacology, School of Medicine, Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
  • Mohsen Taheri Department of Genetics, School of Medicine, Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
  • Fereshteh Heidari Tajabadi Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Mehdi Feizabadi Mail Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Keywords:
Herbal medicine; Hyperoxaluria; Oxalate degrading bacteria; Probiotic bacteria; Sankol drop

Abstract

Background and Objectives: Oxalate degrading bacteria and herbal extracts are new strategy for reducing hyperoxaluria. In Iranian traditional medicine, Sankol oral drop is widely used as an antispasmodic drug to reduce stones from urinary tract. This study aimed to evaluate the synergistic effect of oxalate-degrading bacteria and Sankol oral drop in reducing urinary oxalate in rat model.
Materials and Methods: Several bacterial strains, including Lactobacillus (4), Bifidobacterium (2) and L. paracasei (2) (very strong in degrading oxalate in vitro) were used in this study. Male Wistar rats were divided into 6 groups (n = 6). The rats of Group I received normal diet and drinking water + 60% ethanol (positive group). Groups II (negative group), III, IV, V, and VI rats received diet containing ethylene glycol (3%) for 30 days. Groups III rats received Sankol with minimum concentration (7.5 ml/kg/b.w), Group IV rats received Sankol with maximum concentration (9 ml/kg/b.w), Group V rats received Sankol with minimum concentration + probiotic, and Group VI rats received Sankol with maximum concentration + probiotic for 30 days.
Results: Treatment with Sankol (maximum concentration) and oxalate-degrading probiotic bacteria significantly reduced urinary oxalate (P = .0001). At the end of treatment period, rats in groups II (negative control) showed a high score of CaOx crystal, while rats in VI groups did not show any CaOx crystal.
Conclusion: This is the first study on the simultaneous use of Sankol herbal drop and oxalate-degrading probiotic bacteria that showed a significant reduction in urinary oxalate.

References

1. Barreto FM, Colado Simão AN, Morimoto HK, Batisti Lozovoy MA, Dichi I, Helena da Silva Miglioranza L. Beneficial effects of Lactobacillus plantarum on glycemia and homocysteine levels in postmenopausal women with metabolic syndrome. Nutrition 2014;30:939-942.
2. Ilavenil S, Kim DH, Valan Arasu M, Srigopalram S, Sivanesan R, Choi KC. Phenyl lactic acid from Lactobacillus plantarum promotes adipogenic activity in 3T3-L1 adipocyte via up-regulation of PPAR-c2. Molecules 2015;20:15359-15373.
3. Won TJ, Kim B, Lee Y, Bang JS, Oh ES, Yoo JS, et al. Therapeutic potential of Lactobacillus plantarum CJLP133 for house-dust mite-induced dermatitis in NC/Ngamice. Cell Immunol 2012; 277:49-57.
4. Ahrén IL, Xu J, Önning G, Olsson C, Ahrné S, Molin G. Antihypertensive activity of blueberries fermented by Lactobacillus plantarum DSM 15313 and effects on the gut microbiota in healthy rats. Clin Nutr 2015; 34: 719-726.
5. Dimitrovski D, Cencic A, Winkelhausen E, Langerholc T. Lactobacillus plantarum extracellular metabolites: in vitro assessment of probiotic effects on normal and cancerogenic human cells. Int Dairy J 2014;39: 293-300.
6. Lieske JC, Turner ST, Edeh SN, Ware EB, Kardia SL, Smith JA. Heritability of dietary traits that contribute to nephrolithiasis in a cohort of adult sibships. J Nephrol 2016; 29:45-51.
7. Okombo J, Liebman M. Probiotic induced reduction of gastrointestinal oxalate absorption in healthy subjects. Urol Res 2010;38:169-178.
8. Vijayakumar M, Ilavenil S, Kim DH, Arasu MV, Priya K, Choi KC. In-vitro assessment of the probiotic potential of Lactobacillus plantarum KCC-24 isolated from Italian rye-grass (Loliu mmultiflorum) forage. Anaerobe 2015;32:90-97.
9. Shafaeifar A, Mehrabi S, Malekzadeh J, Jannesar R, Sadeghi H, Vahdani R, et al. Effect of hydrophilic extract of Alhagi Maurorum on Ethylene Glycol-induced renal stone in male wistar rats. Armaghane danesh 2012; 17:129-138.
10. Mohammadi M, Parvaneh E, Tolou-Ghamari Z. Clinical investigation of Levisticum officinale (Lovage) effectiveness’ in patients with cystinuria. J Urol Res 2016;3:1071.
11. Hadjzadeh MA, Khoei A, Hadjzadeh Z, Parizady M. Ethanolic extract of nigella sativa L seeds on ethylene glycol-induced kidney calculi in rats. Urol J 2007;4:86-90.
12. Bahmani M, Baharvand-Ahmadi B, Tajeddini P, Rafieian-Kopaei M, Naghdi N. Identification of medicinal plants for the treatment of kidney and urinary stones. J Renal Inj Prev 2016;5:129-133.
13. Safarinejad MR. Adult urolithiasis in a population-based study in Iran: prevalence, incidence, and associated risk factors. Urol Res 2007;35:73-82.
14. Nikpay S, Moradi K, Azami M, Babashahi M, Otaghi M, Borji M. Frequency of kidney stone different compositions in patients referred to a Lithotripsy Center in Ilam, West of Iran. J Ped Nephrology 2016;4:102-107.
15. Kargar M, Afkari R, Inallo R, Kargar M, Ghorbani-Dalini S. Molecular identification of oxalate-degrading lactobacillus in patients with calcium oxalate urolithiasis. Iran South Med J 2012;15:183-192.
16. Kargar M, Afkari R, Ghorbani-Dalini S. Oxalate-degrading capacities of gastrointestinal lactic acid bacteria and urinary tract stone formation. Zahedan J Res Med Sci 2013; 15:54-58.
17. Jang E, Han J, Kim Y, Kim DH. Lactobacillus plantarum CLP-0611 ameliorates colitis in mice by polarizing M1 to M2-like macrophages. Int Immunopharmacol 2014;21:186-192.
18. Kim SY, Kim SR, Oh MJ, Jung SJ, Kang SY. In vitro antiviral activity of red alga, Polysiphonia morrowii extract and its bromophenols against fish pathogenic infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus. J Microbiol 2011;49: 102-106.
19. Kim JY, Kim H, Jung BJ, Kim NR, Park JE, Chung DK. Lipoteichoic acid isolated from Lactobacillus plantarum suppresses LPS-mediated atherosclerotic plaque inflammation. Mol Cells 2013;35:115-124.
20. Ku HK, Lee H, Choi ID, Ra JH, Kim TY, Jeong JW, et al. Immuno-stimulatory effect of Lactobacillus plantarum HY7712 via toll-like receptor 2 signaling pathway. Cytokine 2014;70: 52-52.
21. Wang K, Li W, Rui X, Chen X, Jiang M, Dong M. Characterization of a novel exopolysaccharide with antitumor activity from Lactobacillus plantarum 70810. Int J Biol Macromol 2014;63:133-139.
22. Stevenson C, Blaauw R, Fredericks E, Visser J, Roux S. Randomized clinical trial: effect of Lactobacillus plantarum 299 v on symptoms of irritable bowel syndrome. Nutrition 2014;30:1151-1157.
23. Amini H, Jahantigh M, Galavi HR, Abdollahi A, Pirouzi A, Afkari R. Evaluation of oxalate-degrading activity and molecular recognition of Oxc, Frc Genes in lactic acid bacterium of inhabit in human colon. Int J Pharm Technol 2016;8:16055-16066.
24. Sasikumar P, Gomathi S, Anbazhagan K, Abhishek A, Paul E, Vasudevan V, et al. Recombinant Lactobacillus plantarum expressing and secreting heterologous oxalate decarboxylase prevents renal calcium oxalate stone deposition in experimental rats. J Biomed Sci 2014; 21:86.
25. Hosseinzadeh H, Khooei AR, Khashayarmanesh Z, Motamed-Shariaty V. Antiurolithiatic activity of Pinus eldarica medw: fruits aqueous extract in rats. Urol J 2010;7:232-237.
26. Khatib N, Dhaval G, Hashilkar N, Rajesh KJ. Antiurolithiatic potential of the fruit extracts of Carica papaya on ethylene glycol induced urolithiatic rats. J Pharm Res 2010;3:2772-2775.
27. Karadi RV, Gadge NB, Alagawadi KR, Savadi RV. Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats. J Ethnopharmacol 2006;105:306-311.
28. Debnath S, Babre N, Manjunath YS, Mallareddy V, Parameshwar P, Hariprasath K. Nephroprotective evaluation of ethanolic extract of the seeds of papaya and pumpkin fruit in Cisplatin-induced Nephrotoxicity. J Pharm Sci Technol 2010;2:241-246.
29. Johri N, Cooper B, Robertson W, Choong S, Rickards D, Unwin R. An update and practical guide to renal stone management. Nephron Clin Pract 2010; 116:c159-171.
30. Zheng W, Wang S. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001; 49: 5165-5170.
31. Durbán A, Abellán JJ, Jiménez-Hernández N, Ponce M, Ponce J, Sala T, et al. Assessing gut microbial diversity from feces and rectal mucosa. Microb Ecol 2011;61: 123-133.
32. Scher JU, Ubeda C, Artacho A, Attur M, Isaac S, Reddy SM, et al. Decreased bacterial diversity characterizes the altered gut microbiota in patients with psoriatic arthritis, resembling dysbiosis in inflammatory bowel disease. Arthritis Rheumatol 2015;67: 128-139.
Published
2020-01-11
How to Cite
1.
Afkari R, Bokaeian M, Dabiri S, Ghaznavi H, Taheri M, Heidari Tajabadi F, Feizabadi MM. Reducing urinary oxalate by simultaneous using Sankol herbal drop with oxalate-degrading bacteria. Iran J Microbiol. 11(6):460-467.
Section
Original Article(s)