Antibacterial potential of essential oils of Zataria multiflora and Mentha piperita, micro- and nano-formulated forms
,
Abstract
Background and Objectives: Plant-derived essential oils (EOs) shave many usages in health and medicine, such as antibacterial agents. The aim of this study was the improvement of antibacterial activities of two EOs using nanotechnology.
Materials and Methods: Antibacterial activity was investigated on four important human pathogenic bacteria using the 96-well plate microdilution method, a quantitative approach. Eleven formulations were prepared using each of the EOs. Eventually, the best nanoformulation with the smallest particle size and polydispersive indices (PDI and SPAN) was selected using each EO for further investigations. Moreover, two microemulsions with similar ingredients and the same portion in comparison with two selected nanoemulsions were also prepared. Antibacterial activity of each EO was compared with its micro- and nano-emulsions.
Results: The antibacterial efficacy of Zataria multiflora EO (ZMEO) was significantly better than Mentha piperita EO (MPEO). Besides, the antibacterial activity of nanoemulsion of ZMEO with a particle size of 129 ± 12 nm was significantly better than no- and micro-formulated forms of ZMEO. Interestingly, the efficiency of MPEO nanoemulsion (160 ± 25 nm) was also significantly better than MPEO and its micro-formulated form.
Conclusion: Regardless of the intrinsic antibacterial property of two examined EOs, by formulating to nanoemulsion, their efficiencies were improved. Nanoemulsion of ZMEO introduced as an inexpensive, potent and green antibacterial agent.
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16. Khani S, Abbasi Sh, Keyhanfar F, Amani A. Use of artificial neural networks for analysis of the factors affecting particle size in mebudipine nanoemulsion. J Biomol Struct Dyn 2019;37:3162-3167.
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19. Aumeeruddy-Elalfi Z, Ismael IS, Hosenally M, Zengin G, Mahomoodally MF. Essential oils from tropical medicinal herbs and food plants inhibit biofilm formation in vitro and are non-cytotoxic to human cells. 3 Biotech 2018;8:395.
20. Utegenova GA, Pallister KB, Kushnarenko SV, Ozek G, Ozek T, Abidkulova KT, et al. Chemical composition and antibacterial activity of essential oils from Ferula L. Species against methicillin-resistant Staphylococcus aureus. Molecules 2018;23:E1679.
21. Trombetta D, Castelli F, Sarpietro MG, Venuti V, Cristani M, Daniele C, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005;49:2474-2478.
22. Botelho MA, Nogueira NA, Bastos GM, Fonseca SG, Lemos TL, Matos FJ, et al. Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. Braz J Med Biol Res 2007;40:349-356.
23. Abyadeh M, Sadroddiny E, Ebrahimi A, Esmaeili F, Saeedi Landi F, Amani A. Electrosprayed chitosan nanoparticles: facile and efficient approach for bacterial transformation. Int Nano Lett 2017;7:291-295.
24. Nirmal NP, Mereddy R, Li L, Sultanbawa Y. Formulation, characterisation and antibacterial activity of lemon myrtle and anise myrtle essential oil in water nanoemulsion. Food Chem 2018;254:1-7.
25. Hussein AM, Mahmoud KF, Hegazy NA, Kamil MM, Mohammad AA, Mehaya FM. Efficiency of micro and nano encapsulated orange peel essential oils on quality of sponge cake. J Environ Sci Tech 2019;12:26-37.
26. Shinoda K, Kunieda H. Conditions to produce so-called microemulsions: Factors to increase the mutual solubility of oil and water by solubilizer. J Colloid Interface Sci 1973;42:381-387.
27. Hemmila MR, Mattar A, Taddonio MA, Arbabi S, Hamouda T, Ward PA, et al. Topical nanoemulsion therapy reduces bacterial wound infection and inflammation after burn injury. Surgery 2010;148:499-509.
28. Anwer MK, Jamil S, Ibnouf EO, Shakeel F. Enhanced antibacterial effects of clove essential oil by nanoemulsion. J Oleo Sci 2014;63:347-354.
29. da Silva Gundel S, de Souza ME, Quatrin PM, Klein B, Wagner R, Gundel A, et al. Nanoemulsions containing Cymbopogon flexuosus essential oil: Development, characterization, stability study and evaluation of antimicrobial and antibiofilm activities. Microb Pathog 2018;118:268-276.
2. Amini SM. Preparation of antimicrobial metallic nanoparticles with bioactive compounds. Mater Sci Eng C Mater Biol Appl 2019;103:109809.
3. Osanloo M, Sedaghat MM, Sereshti H, Rahmani M, Saeedi Landi F, Amani A. Chitosan nanocapsules of tarragon essential oil with low cytotoxicity and long-lasting activity as a green nano-larvicide. J Nanostruct 2019;9:723-725.
4. Osanloo M, Assadpour S, Mehravaran A, Abastabar M, Akhtari J. Niosome-loaded antifungal drugs as an effective nanocarrier system: A mini review. Curr Med Mycol 2018;4:31-36.
5. Osanloo M, Sereshti H, Sedaghat MM, Amani A. Nanoemulsion of Dill essential oil as a green and potent larvicide against Anopheles stephensi. Environ Sci Pollut Res Int 2018;25:6466-6473.
6. Shah P, Bhalodia D, Shelat P. Nanoemulsion: a pharmaceutical review. Sys Rev Pharm 2010;1:24-32.
7. Díaz-Blancas V, Medina D, Padilla-Ortega E, Bortolini-Zavala R, Olvera-Romero M, Luna-Bárcenas G. Nanoemulsion formulations of fungicide tebuconazole for agricultural applications. Molecules 2016;21:E1271.
8. Karthikeyan R, Amaechi BT, Rawls HR, Lee VA. Antimicrobial activity of nanoemulsion on cariogenic Streptococcus mutans. Arch Oral Biol 2011;56:437-445.
9. Sarker DK. Engineering of nanoemulsions for drug delivery. Curr Drug Deliv 2005;2:297-310.
10. Sanei-Dehkordi A, Sedaghat MM, Vatandoost H, Abai MR. Chemical compositions of the peel essential oil of citrus aurantium and its natural larvicidal activity against the malaria vector Anopheles stephensi (Diptera: Culicidae) in comparison with citrus paradisi. J Arthropod Borne Dis 2016;10:577-585.
11. Sanei-Dehkordi A, Gholami S, Abai MR, Sedaghat MM. Essential oil composition and larvicidal evaluation of Platycladus orientalis against two mosquito vectors, Anopheles stephensi and Culex pipiens. J Arthropod Borne Dis 2018;12:101-107.
12. Mohammadi A, Hashemi M, Hosseini SM. Comparison of antifungal activities of various essential oils on the Phytophthora drechsleri, the causal agent of fruit decay. Iran J Microbiol 2015;7:31-37.
13. Moon T, Wilkinson JM, Cavanagh HM. Antiparasitic activity of two Lavandula essential oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata. Parasitol Res 2006;99:722-728.
14. Afshar FF, Saffarian P, Hosseini HM, Sattarian F, Amin M, Fooladi AAI. Antimicrobial effects of Ferula gummosa Boiss gum against extended-spectrum β-lactamase producing Acinetobacter clinical isolates. Iran J Microbiol 2016;8:263-273.
15. Valizadeh A, Shirzad M, Esmaeili F, Amani A. Increased antibacterial activity of cinnamon oil microemulsionin comparison with cinnamon oil bulk and nanoemulsion. Nanomed Res J 2018;3:37-43.
16. Khani S, Abbasi Sh, Keyhanfar F, Amani A. Use of artificial neural networks for analysis of the factors affecting particle size in mebudipine nanoemulsion. J Biomol Struct Dyn 2019;37:3162-3167.
17. Sharififar F, Moshafi M, Mansouri S, Khodashenas M, Khoshnoodi M. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control 2007;18:800-805.
18. İşcan G, Ki̇ri̇mer N, Kürkcüoǧlu Mn, Başer HC, DEMIrci F. Antimicrobial screening of Mentha piperita essential oils. J Agric Food Chem 2002;50:3943-3946.
19. Aumeeruddy-Elalfi Z, Ismael IS, Hosenally M, Zengin G, Mahomoodally MF. Essential oils from tropical medicinal herbs and food plants inhibit biofilm formation in vitro and are non-cytotoxic to human cells. 3 Biotech 2018;8:395.
20. Utegenova GA, Pallister KB, Kushnarenko SV, Ozek G, Ozek T, Abidkulova KT, et al. Chemical composition and antibacterial activity of essential oils from Ferula L. Species against methicillin-resistant Staphylococcus aureus. Molecules 2018;23:E1679.
21. Trombetta D, Castelli F, Sarpietro MG, Venuti V, Cristani M, Daniele C, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005;49:2474-2478.
22. Botelho MA, Nogueira NA, Bastos GM, Fonseca SG, Lemos TL, Matos FJ, et al. Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. Braz J Med Biol Res 2007;40:349-356.
23. Abyadeh M, Sadroddiny E, Ebrahimi A, Esmaeili F, Saeedi Landi F, Amani A. Electrosprayed chitosan nanoparticles: facile and efficient approach for bacterial transformation. Int Nano Lett 2017;7:291-295.
24. Nirmal NP, Mereddy R, Li L, Sultanbawa Y. Formulation, characterisation and antibacterial activity of lemon myrtle and anise myrtle essential oil in water nanoemulsion. Food Chem 2018;254:1-7.
25. Hussein AM, Mahmoud KF, Hegazy NA, Kamil MM, Mohammad AA, Mehaya FM. Efficiency of micro and nano encapsulated orange peel essential oils on quality of sponge cake. J Environ Sci Tech 2019;12:26-37.
26. Shinoda K, Kunieda H. Conditions to produce so-called microemulsions: Factors to increase the mutual solubility of oil and water by solubilizer. J Colloid Interface Sci 1973;42:381-387.
27. Hemmila MR, Mattar A, Taddonio MA, Arbabi S, Hamouda T, Ward PA, et al. Topical nanoemulsion therapy reduces bacterial wound infection and inflammation after burn injury. Surgery 2010;148:499-509.
28. Anwer MK, Jamil S, Ibnouf EO, Shakeel F. Enhanced antibacterial effects of clove essential oil by nanoemulsion. J Oleo Sci 2014;63:347-354.
29. da Silva Gundel S, de Souza ME, Quatrin PM, Klein B, Wagner R, Gundel A, et al. Nanoemulsions containing Cymbopogon flexuosus essential oil: Development, characterization, stability study and evaluation of antimicrobial and antibiofilm activities. Microb Pathog 2018;118:268-276.
| Files | ||
| Issue | Vol 12 No 1 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i1.2517 | |
| Keywords | ||
| Zataria multiflora Mentha piperita Essential oil Antibacterial activity Nanoemulsion | ||
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How to Cite
1.
Osanloo M, Abdollahi A, Valizadeh A, Abedinpour N. Antibacterial potential of essential oils of Zataria multiflora and Mentha piperita, micro- and nano-formulated forms. Iran J Microbiol. 2020;12(1):43-51.
