Increment in protease activity of Lysobacter enzymogenes strain by ultra violet radiation

  • Reyhaneh Jafari Kalahroudi Department of Biological Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran AND Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
  • Vahideh Valizadeh ORCID Mail Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
  • Seyed Mohammad Atyabi ORCID Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
  • Malihe Keramati ORCID Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
  • Reza Ahangari Cohan ORCID Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
  • Atousa Aghai Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran AND Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
  • Dariush Norouzian ORCID Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
Keywords:
Lysobacter enzymogenes;, Protease activity;, Random mutagenesis;, Ultraviolet radiation

Abstract

Background and Objectives: Increasing the amount of protease from microbial sources is in the focus of attention. Random mutagenesis by physical methods like ultraviolet (UV) radiation is a cost effective and convenient procedure for strain improvement. Therefore, in the present study attempts were made to investigate the effect of UV radiation on Lysobacter enzymogenes in order to increase its protease activity.
Materials and Methods: UV mutagenesis was induced in L. enzymogenes fresh culture at the distance of 20 cm from light source for different exposure times of 70, 90, 150 and 200 seconds. The mutated isolates were randomly cultured from the nutrient agar medium to casein agar plate, as a selective medium. The primary screening was performed by observing hydrolysis of casein in the plate and the secondary screening was carried out on skim milk agar on the basis of zone of hydrolysis using bacterial supernatants. Quantification of protease activity was done by Anson’s method using tyrosine as standard.
Results: UV radiation resulted in obtaining 12 mutants out of 100 examined L. enzymogenes strains with increased protease activity. The mutant M2, at 90s exposure time was selected as the best mutant bacterium which produced 1.96 fold more protease over the parent strain.
Conclusion: Random mutation by UV radiation is a simple and convenient method to increase the protease activity of Lysobacter enzymogenes. Furthermore, it seems that the middle time of exposure to UV, 90 s, was the best time because it can induce mutagenesis but did not hamper the bacteria growth and viability.

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Published
2020-12-16
How to Cite
1.
Jafari Kalahroudi R, Valizadeh V, Atyabi SM, Keramati M, Ahangari Cohan R, Aghai A, Norouzian D. Increment in protease activity of Lysobacter enzymogenes strain by ultra violet radiation. Iran J Microbiol. 12(6):601-606.
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