Co-detection of bocavirus and bacteria in a respiratory specimen from a pregnant woman using multiplex real time PCR; a pathogenic role, or a bystander?
,
Abstract
A pregnant woman presented by cough and dyspenia. Employing a respiratory multiplex real-time PCR, Human bocavirus (HBoV), Haemophilus influenza and Staphylococcus aureus were positive at cycle thresholds (CTs) of 21, 35 and 33.5, respectively. The patient was diagnosed for bacterial respiratory infection superimposed by bocavirus due to a relative high CT value. Patient’s condition improved using bronchodilators and corticosteroid without any further antibiotic treatment. HBoV is not exclusively a bystander pathogen in some patients.
1. Jain S, Self WH, Wunderink RG, Fakhran S, Balk R, Bramley AM, et al. Community-acquired Pneumonia requiring hospitalization among U.S. adults. N Engl J Med 2015;373:415-427.
2. Templeton KE, Scheltinga SA, van den Eeden WC, Graffelman AW, van den Broek PJ, Claas EC. Improved diagnosis of the etiology of community-acquired pneumonia with real-time polymerase chain reaction. Clin Infect Dis 2005;41:345-351.
3. Kantola K, Hedman L, Arthur J, Alibeto A, Delwart E, Jartti T, et al. Seroepidemiology of human bocaviruses 1-4. J Infect Dis 2011;204:1403-1412.
4. Hustedt JW, Christie C, Hustedt MM, Esposito D, Vazquez M. Seroepidemiology of human bocavirus infection in Jamaica. PLoS One 2012;7(5):e38206.
5. Guido M, Tumolo MR, Verri T, Romano A, Serio F, De Giorgi M, et al. Human bocavirus: Current knowledge and future challenges. World J Gastroenterol 2016;22:8684-8697.
6. Schildgen O. Human bocavirus: lessons learned to date. Pathogens 2013;2:1-12.
7. Tabasi M, Mokhtari-Azad T, Eshraghian MR, Shadab A, Shatizadeh S, Shafiei-Jandaghi NZ, et al. Human bocavirus infections among children less than two years old in Iran during fall and winter 2012-2013. Iran J Microbiol 2016;8:80-84.
8. Naghipour M, Cuevas LE, Bakhshinejad T, Dove W, Hart CA. Human bocavirus in Iranian children with acute respiratory infections. J Med Virol 2007;79:539-543.
9. Nadji SA, Poos-Ashkan L, Khalilzadeh S, Baghaie N, Shiraghaei MJ, Hassanzad M, et al. Phylogenetic analysis of human bocavirus isolated from children with acute respiratory illnesses and gastroenteritis in Iran. Scand J Infect Dis 2010;42:598-603.
10. Mortazavi HS, Monavari SH, Molaee H, Keyvani H, Pirkooh AA. Human bocavirus in hospitalized Iranian adults with respiratory tract infections during January-June 2014. J Pure Appl Microbiol 2015;9:483-488.
2. Templeton KE, Scheltinga SA, van den Eeden WC, Graffelman AW, van den Broek PJ, Claas EC. Improved diagnosis of the etiology of community-acquired pneumonia with real-time polymerase chain reaction. Clin Infect Dis 2005;41:345-351.
3. Kantola K, Hedman L, Arthur J, Alibeto A, Delwart E, Jartti T, et al. Seroepidemiology of human bocaviruses 1-4. J Infect Dis 2011;204:1403-1412.
4. Hustedt JW, Christie C, Hustedt MM, Esposito D, Vazquez M. Seroepidemiology of human bocavirus infection in Jamaica. PLoS One 2012;7(5):e38206.
5. Guido M, Tumolo MR, Verri T, Romano A, Serio F, De Giorgi M, et al. Human bocavirus: Current knowledge and future challenges. World J Gastroenterol 2016;22:8684-8697.
6. Schildgen O. Human bocavirus: lessons learned to date. Pathogens 2013;2:1-12.
7. Tabasi M, Mokhtari-Azad T, Eshraghian MR, Shadab A, Shatizadeh S, Shafiei-Jandaghi NZ, et al. Human bocavirus infections among children less than two years old in Iran during fall and winter 2012-2013. Iran J Microbiol 2016;8:80-84.
8. Naghipour M, Cuevas LE, Bakhshinejad T, Dove W, Hart CA. Human bocavirus in Iranian children with acute respiratory infections. J Med Virol 2007;79:539-543.
9. Nadji SA, Poos-Ashkan L, Khalilzadeh S, Baghaie N, Shiraghaei MJ, Hassanzad M, et al. Phylogenetic analysis of human bocavirus isolated from children with acute respiratory illnesses and gastroenteritis in Iran. Scand J Infect Dis 2010;42:598-603.
10. Mortazavi HS, Monavari SH, Molaee H, Keyvani H, Pirkooh AA. Human bocavirus in hospitalized Iranian adults with respiratory tract infections during January-June 2014. J Pure Appl Microbiol 2015;9:483-488.
| Files | ||
| Issue | Vol 12 No 1 (2020) | |
| Section | Case Report(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i1.2520 | |
| Keywords | ||
| Multiplex real-time polymerase chain reaction Respiratory co-detection infections Human bocavirus | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Agin K, Rezaee I, Heydarifard Z, Jazayeri S-M. Co-detection of bocavirus and bacteria in a respiratory specimen from a pregnant woman using multiplex real time PCR; a pathogenic role, or a bystander?. Iran J Microbiol. 2020;12(1):70-72.
