Prevalence, genotyping and antibiotic resistance of Listeria monocytogenes and Escherichia coli in fresh beef and chicken meats marketed in Zanjan, Iran
-
Parisa Farhoumand
,
Hassan Hassanzadazar
,
Mohammad Soleiman Soltanpour
,
Majid Aminzare
,
Zahra Abbasi
Abstract
Background and Objectives: The aim of present study was to evaluate the prevalence of Listeria monocytogenes and Escherichia coli, characterization and antimicrobial resistance of their serotypes and genotyping profiles in fresh beef and poultry meats marketed in Zanjan, Iran.
Materials and Methods: A total of 90 (45 chicken and 45 beef) samples were collected from January to June 2018 focusing on retail meat stores of Zanjan city, Iran. Foodborne pathogen detection and antimicrobial resistance of isolates performed by PCR and disc diffusion methods, respectively. Simplex PCR method was used for screening hly and uidA genes in L. monocytogenes and E. coli isolates, respectively.
Results: Findings revealed high contamination in beef and chicken meats with E. coli (68.89% and 88.89%, respectively) and L. monocytogenes (53.33% and 46.67%, respectively). The most likelihood of E. coli isolates belonged to E. coli 13479 serotype. All L. monocytogenes isolates from beef and chicken meat samples had high similarity with serotypes L. monocytogenes strain NCTC 10357 and strain MF 4545, respectively. Multi drug resistance (MDR) was seen in both L. monocytogenes and E. coli isolates.
Conclusion: This study shows an insight of the current status of beef and chicken meat contamination maketed in Zanjan, Iran with E. coli and L. monocytogenes isolates (high contamination rate), their genotypic profile, epidemiological relationship and antimicrobial resistance (AMR) that should be considered as a significant public health concern in Zanjan, Iran.
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17. Paziak-Domańska B, Bogusławska E, Więckowska-Szakiel M, Kotłowski R, Różalska B, Chmiela M, et al. Evaluation of the API test, phosphatidylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. FEMS Microbiol Lett 1999;171:209-214.
18. Wen Y, Wang L, Xu L, Li L, Ren S, Cao C, et al. Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme. Analyst 2016;141:5304-5310.
19. Solomakos N, Govaris A, Koidis P, Botsoglou N. The antimicrobial effect of thyme essential oil, nisin, and their combination against Listeria monocytogenes in minced beef during refrigerated storage. Food Microbiol 2008; 25:120-127.
20. Momtaz H, Jamshidi A. Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: Serogroups, virulence factors, and antimicrobial resistance properties. Poult Sci 2013; 92: 1305-1313.
21. Momtaz H, Safarpoor Dehkordi F, Rahimi E, Ezadi H, Arab R. Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat. Meat Sci 2013; 95: 381-388.
22. Moawad AA, Hotzel H, Awad O, Tomaso H, Neubauer H, Hafez HM, et al. Occurrence of Salmonella enterica and Escherichia coli in raw chicken and beef meat in northern Egypt and dissemination of their antibiotic resistance markers. Gut Pathog 2017;9:57.
23. Soltani J, Poorabbas B, Miri N, Mardaneh J. Health care associated infections, antibiotic resistance and clinical outcome: A surveillance study from Sanandaj, Iran. World J Clin Cases 2016;4:63-70.
24. Kilonzo-Nthenge A, Rotich E, Nahashon S. Evaluation of drug-resistant Enterobacteriaceae in retail poultry and beef. Poult Sci 2013;92:1098-1107.
25. Tozzoli R, Maugliani A, Michelacci V, Minelli F, Caprioli A, Morabito S. Validation on milk and sprouts of EN ISO 16654: 2001-Microbiology of food and animal feeding stuffs-Horizontal method for the detection of Escherichia coli O157. Int J Food Microbiol 2019;288:53-57.
26. Ranjbar R, Halaji M. Epidemiology of Listeria monocytogenes prevalence in foods, animals and human origin from Iran: a systematic review and meta-analysis. BMC Public Health 2018; 18:1057.
27. Fallah A, Saei-Dehkordi, Rahnama M, Tahmasby H, Mahzounieh M. Prevalence and antimicrobial resistance patterns of Listeria species isolated from poultry products marketed in Iran. Food Control 2012;28: 372-332.
28. Arslan S, Baytur S. Prevalence and antimicrobial resistance of Listeria species and subtyping and virulence factors of Listeria monocytogenes from retail meat. J Food Saf 2018; 39(9):e12578.
29. Liu Y, Sun W, Sun T, Gorris LGM, Wang X, Liu B, et al. The prevalence of Listeria monocytogenes in meat products in China: A systematic literature review and novel meta-analysis approach. Int J Food Microbiol 2020; 312: 108358.
30. Islam MS, Husna AA, Islam MA, Khatun MM. Prevalence of Listeria monocytogenes in Beef, Chevon and Chicken in Bangladesh. Am J Food Sci Health 2016; 2: 39-44.
31. Hamidiyan N, Salehi-Abargouei A, Rezaei Z, Dehghani Tafti R, Akrami-Mohajeri F. The prevalence of Listeria spp. food contamination in Iran: A systematic review and meta-analysis. Food Res Int 2018; 107:437-450.
32. Maktabi S, Pourmehdi M, Zarei M, Moalemian R. Occurrence and antibiotic resistance of Listeria monocytogenes in retail minced beef distributed in Ahvaz, South-West of Iran. J Food Qual Hazards Control 2015;2:101-106.
33. Pesavento G, Ducci B, Nieri D, Comodo N, Nostro AL. Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control 2010;21:708-713.
34. Rosselli R, Romoli O, Vitulo N, Vezzi A, Campanaro S, De Pascale F, et al. Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon. Sci Rep 2016;6:32165.
2. Şanlıbaba P, Tezel BU, Çakmak GA. Prevalence and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat foods in Turkey. J Food Qual 2018;2018: 7693782.
3. Mitchell NM, Johnson JR, Johnston B, Curtiss R, Mellata M. Zoonotic potential of Escherichia coli isolates from retail chicken meat products and eggs. Appl Environ Microbiol 2015;81:1177-1187.
4. Dallal MMS, Doyle MP, Rezadehbashi M, Dabiri H, Sanaei M, Modarresi S, et al. Prevalence and antimicrobial resistance profiles of Salmonella serotypes, Campylobacter and Yersinia spp. isolated from retail chicken and beef, Tehran, Iran. Food Control 2010;21:388-392.
5. Sonnier JL, Karns JS, Lombard JE, Kopral CA, Haley BJ, Kim S-W, et al. Prevalence of Salmonella enterica, Listeria monocytogenes, and pathogenic Escherichia coli in bulk tank milk and milk filters from US dairy operations in the National Animal Health Monitoring System Dairy 2014 study. J Dairy Sci 2018;101:1943-1956.
6. Campion A, Morrissey R, Field D, Cotter PD, Hill C, Ross RP. Use of enhanced nisin derivatives in combination with food-grade oils or citric acid to control Cronobacter sakazakii and Escherichia coli O157: H7. Food Microbiol 2017;65:254-263.
7. Zhao C, Ge B, De Villena J, Sudler R, Yeh E, Zhao S, et al. Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, DC, area. Appl Environ Microbiol 2001;67:5431-5436.
8. Godambe LP, Bandekar J, Shashidhar R. Species specific PCR based detection of Escherichia coli from Indian foods. 3 Biotech 2017; 7:130.
9. Poimenidou SV, Dalmasso M, Papadimitriou K, Fox EM, Skandamis PN, Jordan K. Virulence gene sequencing highlights similarities and differences in sequences in Listeria monocytogenes serotype 1/2a and 4b strains of clinical and food origin from 3 different geographic locations. Front Microbiol 2018; 9: 1103.
10. Molina F, López-Acedo E, Tabla R, Roa I, Gómez A, Rebollo JE. Improved detection of Escherichia coli and coliform bacteria by multiplex PCR. BMC Biotechnol 2015; 15:48.
11. Kačániová M, Kluz M, Petrová J, Mellen M, Kunová S. Incidence of Listeria monocytogenes in meat product samples by real time PCR. Mod Chem Appl 2015;3:2.
12. Köppel R, Tolido I, Marti G, Peier M. Detection of DNA from Escherichia coli, Clostridium perfringens, Staphylococcus aureus and Bacillus cereus after simplified enrichment using a novel multiplex real-time PCR system. Eur Food Res Technol 2017;243:521-530.
13. Tao T, Chen Q, Bie X, Lu F, Lu Z. Investigation on prevalence of Listeria spp. and Listeria monocytogenes in animal-derived foods by multiplex PCR assay targeting novel genes. Food Control 2017;73:704-711.
14. ISIRI (Institute of Standards & Industrial Research of Iran) 2946, Microbiology of food and animal feeding stuffs -Detection and enumeration of presumptive Escherichia coli -Most probable number technique. 2011.
15. Safarpor dehkordi F, Yahaghi E, Darian E. Prevalence of antibiotic resistance in Escherichia coli isolated from poultry meat supply in Isfahan. Iran J Med Microbiol 2014; 8:41-47.
16. Karimiazar F, Soltanpour MS, Aminzare M, Hassanzadazar H. Prevalence, genotyping, serotyping, and antibiotic resistance of isolated Salmonella strains from industrial and local eggs in Iran. J Food Saf 2019;39(1):e12585.
17. Paziak-Domańska B, Bogusławska E, Więckowska-Szakiel M, Kotłowski R, Różalska B, Chmiela M, et al. Evaluation of the API test, phosphatidylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. FEMS Microbiol Lett 1999;171:209-214.
18. Wen Y, Wang L, Xu L, Li L, Ren S, Cao C, et al. Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme. Analyst 2016;141:5304-5310.
19. Solomakos N, Govaris A, Koidis P, Botsoglou N. The antimicrobial effect of thyme essential oil, nisin, and their combination against Listeria monocytogenes in minced beef during refrigerated storage. Food Microbiol 2008; 25:120-127.
20. Momtaz H, Jamshidi A. Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: Serogroups, virulence factors, and antimicrobial resistance properties. Poult Sci 2013; 92: 1305-1313.
21. Momtaz H, Safarpoor Dehkordi F, Rahimi E, Ezadi H, Arab R. Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat. Meat Sci 2013; 95: 381-388.
22. Moawad AA, Hotzel H, Awad O, Tomaso H, Neubauer H, Hafez HM, et al. Occurrence of Salmonella enterica and Escherichia coli in raw chicken and beef meat in northern Egypt and dissemination of their antibiotic resistance markers. Gut Pathog 2017;9:57.
23. Soltani J, Poorabbas B, Miri N, Mardaneh J. Health care associated infections, antibiotic resistance and clinical outcome: A surveillance study from Sanandaj, Iran. World J Clin Cases 2016;4:63-70.
24. Kilonzo-Nthenge A, Rotich E, Nahashon S. Evaluation of drug-resistant Enterobacteriaceae in retail poultry and beef. Poult Sci 2013;92:1098-1107.
25. Tozzoli R, Maugliani A, Michelacci V, Minelli F, Caprioli A, Morabito S. Validation on milk and sprouts of EN ISO 16654: 2001-Microbiology of food and animal feeding stuffs-Horizontal method for the detection of Escherichia coli O157. Int J Food Microbiol 2019;288:53-57.
26. Ranjbar R, Halaji M. Epidemiology of Listeria monocytogenes prevalence in foods, animals and human origin from Iran: a systematic review and meta-analysis. BMC Public Health 2018; 18:1057.
27. Fallah A, Saei-Dehkordi, Rahnama M, Tahmasby H, Mahzounieh M. Prevalence and antimicrobial resistance patterns of Listeria species isolated from poultry products marketed in Iran. Food Control 2012;28: 372-332.
28. Arslan S, Baytur S. Prevalence and antimicrobial resistance of Listeria species and subtyping and virulence factors of Listeria monocytogenes from retail meat. J Food Saf 2018; 39(9):e12578.
29. Liu Y, Sun W, Sun T, Gorris LGM, Wang X, Liu B, et al. The prevalence of Listeria monocytogenes in meat products in China: A systematic literature review and novel meta-analysis approach. Int J Food Microbiol 2020; 312: 108358.
30. Islam MS, Husna AA, Islam MA, Khatun MM. Prevalence of Listeria monocytogenes in Beef, Chevon and Chicken in Bangladesh. Am J Food Sci Health 2016; 2: 39-44.
31. Hamidiyan N, Salehi-Abargouei A, Rezaei Z, Dehghani Tafti R, Akrami-Mohajeri F. The prevalence of Listeria spp. food contamination in Iran: A systematic review and meta-analysis. Food Res Int 2018; 107:437-450.
32. Maktabi S, Pourmehdi M, Zarei M, Moalemian R. Occurrence and antibiotic resistance of Listeria monocytogenes in retail minced beef distributed in Ahvaz, South-West of Iran. J Food Qual Hazards Control 2015;2:101-106.
33. Pesavento G, Ducci B, Nieri D, Comodo N, Nostro AL. Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control 2010;21:708-713.
34. Rosselli R, Romoli O, Vitulo N, Vezzi A, Campanaro S, De Pascale F, et al. Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon. Sci Rep 2016;6:32165.
| Files | ||
| Issue | Vol 12 No 6 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v12i6.5028 | |
| Keywords | ||
| Antibiotic resistance; Escherichia coli; Genotyping; Listeria monocytogenes; Meat hygiene | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Farhoumand P, Hassanzadazar H, Soltanpour MS, Aminzare M, Abbasi Z. Prevalence, genotyping and antibiotic resistance of Listeria monocytogenes and Escherichia coli in fresh beef and chicken meats marketed in Zanjan, Iran. Iran J Microbiol. 2020;12(6):537-546.
