The effect of zinc supplementation on pro-inflammatory cytokines (TNF-α, IL-1 AND IL-6) in mice with Escherichia coli LPS-induced diarrhea

  • Sulaiman Yusuf Department of Paediatric, School of Medicine, Universitas Syiah Kuala, Aceh, Indonesia; Department of Paediatric, Dr. Zainoel Abidin Hospital, Aceh, Indonesia
  • Yati Soenarto Department of Paediatric, School of Medicine, Gajah Mada University, Yogyakarta, Indonesia
  • Muhammad Juffrie Department of Paediatric, School of Medicine, Gajah Mada University, Yogyakarta, Indonesia
  • Wiryatun Lestariana Department of Biochemistry, School of Medicine, Gajah Mada University, Yogyakarta, Indonesia
Zinc; Tumor necrosis factor-α; Interleukin 1; Interleukin 6; Diarrhea


Background and Objectives: Inflammation in the intestine causes diarrhea due to an increased release of pro-inflammatory cytokines such as TNF-α, IL-1, and IL-6. These are triggered by the exposure of E. coli-LPS to epithelial cells of the intestinal mucosa as well as low concentration of zinc in plasma such as in infants or children who are experiencing diarrhea. This paper aims to determine the effects of zinc supplementation on pro-inflammatory cytokines (TNF-α, IL-1 and IL-6) in mice with E. coli-LPS-induced diarrhea.
Materials and Methods: This study used a controlled trial experimental design in the laboratory. A sample size of 20 mice were randomly divided into 4 groups: 1) Control group was given standard foods, 2) Trial group was given E. coli-LPS 2.5 mg/kg/oral once on day1, 3) Prevention group was given E. coli-LPS + 30 mg/kg/oral of zinc once daily for 12 days, 4) Therapeutic group was given E. coli-LPS, and were then given 30 mg/kg/oral of zinc once daily for 12 days if diarrhea occurred. Blood samples of mice were taken through the orbital sinus on the 0, 5th, 10th hour, and on the 4th, 8th and 12th days.
Results: Positive effects of zinc supplementation on levels of pro-inflammatory cytokines were observed, in which the higher levels of zinc were present, the lower levels of pro-inflammatory cytokines, especially TNF-α were observed. However, there was an increase of IL-1 and IL-6 levels on the 8th day in the prevention and therapeutic groups.
Conclusion: Oral zinc supplementation had a significant positive effect on the levels of pro-inflammatory cytokines. Where there were higher levels of zinc, lower levels of pro-inflammatory cytokines TNF-α were present.


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How to Cite
Yusuf S, Soenarto Y, Juffrie M, Lestariana W. The effect of zinc supplementation on pro-inflammatory cytokines (TNF-α, IL-1 AND IL-6) in mice with Escherichia coli LPS-induced diarrhea. Iran J Microbiol. 11(5):412-418.
Original Article(s)