Isolation and screening of Thermoactinomycetaceae family members as an extremophilic poor investigated and promising natural source of antimicrobial substances
Abstract
Background and Objectives: Recent evidences have shown that methicillin-resistant Staphylococcus aureus (MRSA) can cause severe infections and is resistant to almost all commercially available antibiotics. Therefore, screening unknown sources of biological compounds such as the Thermoactinomycetaceae family as extremophilic bacteria may be helpful to find new antimicrobial agents.
Materials and Methods: Various samples were collected from different ecosystems, including desert, volcano, compost, and forest. They were cultured on Soil extract agar and Water agar. The antimicrobial activity of the isolates was evaluated using agar overlay and well diffusion methods. Members of the Thermoactinomycetaceae family were selected for further study: Their ability to grow at different temperatures, NaCl concentrations, and pH values, enzyme production ability, antimicrobial secondary screening, fractionation of their supernatants and so on.
Results: According to molecular identification of active isolates against MRSA, three strains, including Laceyella sacchari UTMC 2705, Thermoactinomyces sp. UTMC 2721, and Laceyella sp. UTMC 2731, belonged to Thermoactinomycetaceae were identified. The minimum inhibitory concentrations of their extracts were tested against some pathogenic bacteria, showing their antimicrobial activity with a broad spectrum. The results of TLC bioautography of the extracts showed that the most active fractions were semi-polar. Also, the results of HPLC analysis showed the existence of several UV-active compounds in their extracts.
Conclusion: The present study highlighted the importance and potential of Thermoactinomycetaceae members as a less-known source of antibiotics against pathogenic bacteria.
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22. Maurya IK, Dilawari R, Singh D, Singh RP (2020). Bioactive Compounds from Extremophiles. In Microbial Versatility in Varied Environments. Springer, Singapore. pp. 1-19.
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25. Henciya S, Vengateshwaran TD, Gokul MS, Dahms HU, James RA. Antibacterial activity of halophilic bacteria against drug-resistant microbes associated with diabetic foot infections. Curr Microbiol 2020; 77: 3711-3723.
26. Fuzzati N. Analysis methods of ginsenosides. J Chromatogr B Analyt Technol Biomed Life Sci 2004; 812: 119-133.
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28. Teta R, Marteinsson VT, Longeon A, Klonowski AM, Groben R, Bourguet-Kondracki ML, et al. Thermoactinoamide A, an antibiotic lipophilic cyclopeptide from the icelandic thermophilic bacterium Thermoactinomyces vulgaris. J Nat Prod 2017; 80: 2530-2535.
29. Frikha Dammak D, Zarai Z, Najah S, Abdennabi R, Belbahri L, Rateb ME, et al. Antagonistic properties of some halophilic thermoactinomycetes isolated from superficial sediment of a solar saltern and production of cyclic antimicrobial peptides by the novel isolate Paludifilum halophilum. Biomed Res Int 2017; 2017: 1205258.
30. Frikha-Dammak D, Fakhfakh J, Belhaj D, Bouattour E, Ayadi H, Chaabouni M, et al. Enhancement of antibacterial activity of Paludifilum halophilum and identification of N-(1-Carboxy-ethyl)-phthalamic acid as the main bioactive compound. Biomed Res Int 2020; 2020: 4805706.
31. Yao S, Liu Y, Zhang M, Zhang X, Li H, Zhao T, et al. Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature Daqu. Int J Syst Evol Microbiol 2014; 64: 206-210.
32. Yao S, Xu Y, Xin C, Xu L, Liu Y, Li H, et al. Genome sequence of Thermoactinomyces daqus H-18, a novel thermophilic species isolated from high-temperature Daqu. Genome Announc 2015; 3(1): e01394-14.
33. Uzel A, Kocabaş EE, Bedir E. Prevalence of Thermoactinomyces thalpophilus and T. sacchari strains with biotechnological potential at hot springs and soils from West Anatolia in Turkey. Turkish J Biol 2011; 35: 195-202.
34. Akiyama H, Oku N, Kasai H, Shizuri Y, Matsumoto S, Igarashi Y. Metabolites from thermophilic bacteria I: N-propionylanthranilic acid, a co-metabolite of the bacillamide class antibiotics and tryptophan metabolites with herbicidal activity from Laceyella sacchari. J Antibiot (Tokyo) 2014; 67: 795-798.
2. Horváth A, Dobay O, Sahin-Tóth J, Juhász E, Pongrácz J, Iván M, et al. Characterisation of antibiotic resistance, virulence, clonality and mortality in MRSA and MSSA bloodstream infections at a tertiary-level hospital in Hungary: a 6-year retrospective study. Ann Clin Microbiol Antimicrob 2020; 19: 17.
3. Laxminarayan R, Duse A, Wattal C, Zaidi AKM, Wertheim HFL, Sumpradit N, et al. Antibiotic resistance-the need for global solutions. Lancet Infect Dis 2013; 13: 1057-1098.
4. Gualerzi CO, Brandi L, Fabbretti A, Pon CL. Antibiotics: Targets, mechanisms and resistance. Germany. Wiley-VCH (2013).
5. Chen Y, Ntai I, Ju K-S, Unger M, Zamdborg L, Robinson SJ, et al. A proteomic survey of nonribosomal peptide and polyketide biosynthesis in actinobacteria. J Proteome Res 2012; 11: 85-94.
6. Giddings L-A, Newman DJ (2015). Bioactive Compounds from Terrestrial Extremophiles. Springer Cham Heidelberg New York Dordrecht London. pp.1-75.
7. Wang K-X, He Y-Q, Chen R-W, Li C, Tian X-P, Long L-J. Staphylospora marina gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal vent in the Pacific Ocean. Int J Syst Evol Microbiol 2019; 69: 1452-1458.
8. Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F (2014). The Prokaryotes: Firmicutes and Tenericutes. Springer. Rosenberg.
9. Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey F, et al (2009). Bergey's Manual of Systematic Bacteriology: Volume 3: The Firmicutes. Springer Science & Business Media.
10. Chaudhary HS, Yadav J, Shrivastava AR, Singh S, Singh AK, Gopalan N. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). J Adv Pharm Technol Res 2013; 4: 118-123.
11. Patel JB (2017). Performance Standards for Antimicrobial Susceptibility Testing (27 ed.). United States: Clinical and Laboratory Standards Institute.
12. Gebreyohannes G, Moges F, Sahile S, Raja N. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pac J Trop Biomed 2013; 3: 426-435.
13. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016; 6: 71-79.
14. Babavalian H, Amoozegar MA, Pourbabaee AA, Moghaddam MM, Shakeri F. Isolation and identification of moderately halophilic bacteria producing hydrolytic enzymes from the largest hypersaline playa in Iran. Microbiology 2013; 82: 466-474.
15. Kumar D, Kumar L, Nagar S, Raina C, Parshad R, Gupta VK. Screening, isolation and production of lipase/esterase producing Bacillus sp. strain DVL2 and its potential evaluation in esterification and resolution reactions. Arch Appl Sci Res 2012; 4: 1763-1770.
16. Almasi F, Mohammadipanah F, Adhami H-R, Hamedi J. Introduction of marine‐derived Streptomyces sp. UTMC 1334 as a source of pyrrole derivatives with anti‐acetylcholinesterase activity. J Appl Microbiol 2018; 125: 1370-1382.
17. Hamedi J, Papiran R, Moghimi H. Isolation and screening of phytotoxin-producing actinomycetes for biological control of Cardaria draba. Prog Biol Sci 2014; 4: 113-121.
18. Apu A, Muhit M, Tareq S, Pathan A, Jamaluddin A, Ahmed M. Antimicrobial Activity and Brine Shrimp Lethality Bioassay of the Leaves Extract of Dillenia indica Linn. J Young Pharm 2010; 2: 50-53.
19. Dewanjee S, Gangopadhyay M, Bhattacharya N, Khanra R, Dua TK. Bioautography and its scope in the field of natural product chemistry. J Pharm Anal 2015; 5: 75-84.
20. Shivlata L, Tulasi S. Thermophilic and alkaliphilic Actinobacteria: biology and potential applications. Front Microbiol 2015; 6: 1014.
21. Jayaweera JA, Karunarathne M, Kumbukgolla WW. The importance of timely introduction of vancomycin therapy against methicillin-resistant Staphylococcus aureus (MRSA) bacteremia and severity of MRSA bacteremia at Teaching Hospital, Anuradhapura, Sri Lanka. Int J One Health 2017; 3: 7-11.
22. Maurya IK, Dilawari R, Singh D, Singh RP (2020). Bioactive Compounds from Extremophiles. In Microbial Versatility in Varied Environments. Springer, Singapore. pp. 1-19.
23. Núñez-Montero K, Barrientos L. Advances in Antarctic research for antimicrobial discovery: a comprehensive narrative review of bacteria from Antarctic environments as potential sources of novel antibiotic compounds against human pathogens and microorganisms of industrial importance. Antibiotics (Basel) 2018; 7: 90.
24. Mahajan GB, Balachandran L. Sources of antibiotics: Hot springs. Biochem Pharmacol 2017; 134: 35-41.
25. Henciya S, Vengateshwaran TD, Gokul MS, Dahms HU, James RA. Antibacterial activity of halophilic bacteria against drug-resistant microbes associated with diabetic foot infections. Curr Microbiol 2020; 77: 3711-3723.
26. Fuzzati N. Analysis methods of ginsenosides. J Chromatogr B Analyt Technol Biomed Life Sci 2004; 812: 119-133.
27. Bulkley D, Johnson F, Steitz TA. The antibiotic thermorubin inhibits protein synthesis by binding to inter-subunit bridge B2a of the ribosome. J Mol Biol 2012; 416: 571-578.
28. Teta R, Marteinsson VT, Longeon A, Klonowski AM, Groben R, Bourguet-Kondracki ML, et al. Thermoactinoamide A, an antibiotic lipophilic cyclopeptide from the icelandic thermophilic bacterium Thermoactinomyces vulgaris. J Nat Prod 2017; 80: 2530-2535.
29. Frikha Dammak D, Zarai Z, Najah S, Abdennabi R, Belbahri L, Rateb ME, et al. Antagonistic properties of some halophilic thermoactinomycetes isolated from superficial sediment of a solar saltern and production of cyclic antimicrobial peptides by the novel isolate Paludifilum halophilum. Biomed Res Int 2017; 2017: 1205258.
30. Frikha-Dammak D, Fakhfakh J, Belhaj D, Bouattour E, Ayadi H, Chaabouni M, et al. Enhancement of antibacterial activity of Paludifilum halophilum and identification of N-(1-Carboxy-ethyl)-phthalamic acid as the main bioactive compound. Biomed Res Int 2020; 2020: 4805706.
31. Yao S, Liu Y, Zhang M, Zhang X, Li H, Zhao T, et al. Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature Daqu. Int J Syst Evol Microbiol 2014; 64: 206-210.
32. Yao S, Xu Y, Xin C, Xu L, Liu Y, Li H, et al. Genome sequence of Thermoactinomyces daqus H-18, a novel thermophilic species isolated from high-temperature Daqu. Genome Announc 2015; 3(1): e01394-14.
33. Uzel A, Kocabaş EE, Bedir E. Prevalence of Thermoactinomyces thalpophilus and T. sacchari strains with biotechnological potential at hot springs and soils from West Anatolia in Turkey. Turkish J Biol 2011; 35: 195-202.
34. Akiyama H, Oku N, Kasai H, Shizuri Y, Matsumoto S, Igarashi Y. Metabolites from thermophilic bacteria I: N-propionylanthranilic acid, a co-metabolite of the bacillamide class antibiotics and tryptophan metabolites with herbicidal activity from Laceyella sacchari. J Antibiot (Tokyo) 2014; 67: 795-798.
| Files | ||
| Issue | Vol 15 No 1 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v15i1.11920 | |
| Keywords | ||
| Antibiotic resistance; Bioactive compound; Extremophiles; Isolation; Methicillin-resistant Staphylococcus aureus; Thermoactinomycetaceae | ||
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How to Cite
1.
Seyed Shirazi SR, Hamedi J. Isolation and screening of Thermoactinomycetaceae family members as an extremophilic poor investigated and promising natural source of antimicrobial substances. Iran J Microbiol. 2023;15(1):69-78.
