Frequency of adenovirus serotype 8 in patients with Keratoconjunctivitis, in Ahvaz, Iran
-
Kolsoom Shafiei
,
Manoochehr Makvandi
,
Ali Teimoori
,
Alireza Samarbafzadeh
,
Gholamreza Khataminia
,
Shahram Jalilian
,
Niloofar Neisi
,
Kimia Makvandi
,
Mehrdad Sadeghi Haj
Abstract
Background and Objectives: Adenoviral keratoconjunctivitis is an extremely frequent ophthalmological disease caused by various serological subtypes of human adenovirus (HAdV) worldwide. Adenoviruses serotypes 8, 11, 19, 37 frequently cause epidemic keratoconjunctivitis (EKC). This study was conducted to evaluate the frequency of adenovirus serotypes in patients with EKC in Ahvaz , Iran.
Materials and Methods: Eighty- eight ocular swabs were collected from patients with EKC. The specimens were analyzed for detection of adenovirus by standard PCR. The PCR products were further sequenced and analyzed to determine the serotypes.
Results: The study population consisted of 49/88 (55.7%) males and 39/88 (44.3%) females. Among them 25 (51.02%) males and 22 (56.41%) females were positive for HAdV serotype 8 (p= 0.488). Overall forty-seven (53.4%) samples were positive for AdV serotype 8 while forty- one patients (46.59%) were negative for the adenovirus serotypes.
Conclusion: The results of this study revealed predominanance of HAdV 8 with high prevalence of 53.4% among patients with Keratoconjunctivitis. Forty- one patients (46.59%) were negative for adenovirus. Still, the role for other related viruses such as enteroviruses need to be investigated in patients with EKC.
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12. Trinavarat A, Atchaneeyasakul L-o. Treatment of epidemic Keratoconjunctivitis with 2% povidone–iodine: a pilot study. J Ocul Pharmacol Ther 2012;28:53-58.
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16. Pinto RDP, Lira RPC, Arieta CEL, Castro RSd, Bonon SHA. The prevalence of adenoviral conjunctivitis at the clinical hospital of the state university of campinas. Clinics (Sao Paulo) 2015;70:748-750.
17. Allard A, Albinsson B, Wadell G. Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis. J Clin Microbiol 2001;39:498-505.
18. Lee Y-C, Chen N, Huang I-T, Yang H-H, Huang C-T, Chen L-K, et al. Human adenovirus type 8 epidemic keratoconjunctivitis with large corneal epithelial full-layer detachment: an endemic outbreak with uncommon manifestations. Clin Ophthalmol 2015;9:953-957.
19. Sohrabi M, Goodarzi Z, Saberfar E, Lashini H. The prevalence of viral conjunctivitis in patients who referred to eye specialist hospitals in Tehran, Iran. Iran J Ophthalmol 2014;26:29-32.
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21. Goudarzi H, Rostami S, Eslami G, Rahbar AS, Miraghasi F, Besharat M, et al. Frequency of adenoviral conjunctivitis by cell culture and PCR method in two referral university hospitals in Tehran. Iran J Clin Infect Dis 2006;1:127-129.
22. Huang G, Yao W, Yu W, Mao L, Sun H, Yao W, et al. Outbreak of epidemic keratoconjunctivitis caused by human adenovirus type 56, China, 2012. PLoS One 2014;9(10):e110781.
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24. Ishiko H, Shimada Y, Konno T, Hayashi A, Ohguchi T, Tagawa Y, et al. Novel human adenovirus causing nosocomial epidemic keratoconjunctivitis. J Clin Microbiol 2008;46:2002-2008.
25. Kaneko H, Ishiko H, Ohguchi T, Tagawa Y, Aoki K, Suzutani T, et al. Nucleotide sequence variation in the hexon gene of human adenovirus type 8 and 37 strains from epidemic keratoconjunctivitis patients in Japan. J Gen Virol 2009;90:2260-2265.
26. Nakamura M, Hirano E, Kowada K, Ishiguro F, Yamagishi Z, Adhikary AK, et al. Surveillance of adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 1995–2010. J Med Virol 2012;84:81-86.
27. Centers for Disease Control and Prevention. Adenovirus-associated epidemic keratoconjunctivitis outbreaks - four states, 2008-2010. Morb Mortal Wkly Rep 2013;62:637-641.
28. Yağci R, Akçali A, Yağci S, Konno T, Ishiko H, Duman S, et al. Molecular identification of adenoviral conjunctivitis in Turkey. Eur J Ophthalmol 2010;20:669-674.
29. Jin X-H, Ishiko H, Ha NT, Ohguchi T, Akanuma M, Aoki K, et al. Molecular epidemiology of adenoviral conjunctivitis in Hanoi, Vietnam. Am J Ophthalmol 2006;142:1064-1066.
30. Aoki K, Kato M, Ohtsuka H, Ishii K, Nakazono N, Sawada H. Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus types 4 and 19 infections. Br J Ophthalmol 1982;66:776-780.
31. Ariga T, Shimada Y, Ohgami K, Tagawa Y, Ishiko H, Aoki K, et al. New genome type of adenovirus serotype 4 caused nosocomial infections associated with epidemic conjunctivitis in Japan. J Clin Microbiol 2004;42:3644-3648.
32. Saitoh-Inagawa W, Oshima A, Aoki K, Itoh N, Isobe K, Uchio E, et al. Rapid diagnosis of adenoviral conjunctivitis by PCR and restriction fragment length polymorphism analysis. J Clin Microbiol 1996;34:2113-2116.
33. Tabbara KF, Omar N, Hammouda E, Akanuma M, Ohguchi T, Ariga T, et al. Molecular epidemiology of adenoviral keratoconjunctivitis in Saudi Arabia. Mol Vis 2010; 16: 2132-2136.
34. Ishii K, Nakazono N, Fujinaga K, Fujii S, Kato M, Ohtsuka H, et al. Comparative studies on aetiology and epidemiology of viral conjunctivitis in three countries of East Asia—Japan, Taiwan and South Korea. Int J Epidemiol 1987;16:98-103.
35. Massey J, Henry R, Minnich L, Lamson DM, St George K. Notes from the Field. Health care–associated outbreak of epidemic keratoconjunctivitis — west Virginia, 2015. MMWR Morb Mortal Wkly Rep 2016;65:382-383.
36. Rutala WA, Peacock JE, Gergen MF, Sobsey MD, Weber DJ. Efficacy of hospital germicides against adenovirus 8, a common cause of epidemic keratoconjunctivitis in health care facilities. Antimicrob Agents Chemother 2006;50:1419-1424.
37. Shukla D, Kumar A, Srivastava S, Dhole TN. Molecular identification and phylogenetic study of coxsackievirus A24 variant isolated from an outbreak of acute hemorrhagic conjunctivitis in India in 2010. Arch Virol 2013;158:679-684.
38. Lévêque N, Huguet P, Norder H, Chomel JJ. Enteroviruses responsible for acute hemorrhagic conjunctivitis.Med Mal Infect 2010;40:212-218.
2. Stefkovicova M, Vicianova V, Sokolik J, Madar R. Causes and control measures in hospital outbreaks of epidemic keratoconjunctivitis. Indoor Built Environ 2006;15:111-115.
3. González-López J, Morcillo-Laiz R, Muñoz-Negrete F. Adenoviral keratoconjunctivitis: an update. Arch Soc Esp Oftalmol 2013;88:108-115.
4. Erdin BN, Pas SD, Durak İ, Schutten M, Sayıner AA. A 5-year study of adenoviruses causing conjunctivitis in Izmir, Turkey. J Med Virol 2015;87:472-477.
5. Ghebremedhin B. Human adenovirus: Viral pathogen with increasing importance. Eur J Microbiol Immunol (Bp) 2014;4:26-33.
6. Lu X, Erdman D. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Arch Virol 2006;151:1587-1602.
7. Jay R, Radkea, James L. Cook a human adenovirus infections: update and consideration of mechanisms of viral persistence. Curr Opin Infect Dis 2018 ; 31: 251-256.
8. Okada M, Ogawa T, Kubonoya H, Yoshizumi H, Shinozaki K. Detection and sequence-based typing of human adenoviruses using sensitive universal primer sets for the hexon gene. Arch Virol 2007;152:1-9.
9. Shamsi-Shahrabadi M, Mousavi E, Monavari S, Ataei-Pirkooh A, Bakhtiari P. Incidence of adenoviral conjunctivitis in patients referred to the Iran University affiliated hospital. Iran J Virol 2009; 3:7-11.
10. Takashi U, Hironori M, Tomohiro U, Tomoko TK, Yusuke S, Tsuguto F, et al. Clinical and virological analysis of epidemic keratoconjunctivitis caused by adenovirus type 54 in a regional ophthalmic clinic in Kyushu, Japan. Clin Ophthalmol 2018;12:511-517.
11. Majeed A, Naeem Z, Khan DA, Ayaz A. Epidemic adenoviral conjunctivitis report of an outbreak in a military garison and recommendations for its management and prevention. J Pak Med Assoc 2005;55:273-275.
12. Trinavarat A, Atchaneeyasakul L-o. Treatment of epidemic Keratoconjunctivitis with 2% povidone–iodine: a pilot study. J Ocul Pharmacol Ther 2012;28:53-58.
13. Frantzidou F, Pavlitou A, Mataftsi A, Dumaidi K, Georgiadis N. Molecular epidemiology of adenovirus strains isolated from patients with ocular disease in the area of Thessaloniki, Greece (1998-2002). J Med Virol 2005;75:440-446.
14. Hongling Q, Xiao L, Xingui T, Zhichao Z, Ke X, Haitao L, et al. Serotype-specific neutralizing antibody epitopes of human adenovirus type 3 (HAdV-3) and HAdV-7 reside in multiple hexon hypervariable regions. J Virol 2012;86: 7964-7975.
15. Akçay E, Çarhan A, Hondur G, Tufan ZK, Duru N, Kılıç S, et al. Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study. Braz J Infect Dis 2017;21:391-395.
16. Pinto RDP, Lira RPC, Arieta CEL, Castro RSd, Bonon SHA. The prevalence of adenoviral conjunctivitis at the clinical hospital of the state university of campinas. Clinics (Sao Paulo) 2015;70:748-750.
17. Allard A, Albinsson B, Wadell G. Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis. J Clin Microbiol 2001;39:498-505.
18. Lee Y-C, Chen N, Huang I-T, Yang H-H, Huang C-T, Chen L-K, et al. Human adenovirus type 8 epidemic keratoconjunctivitis with large corneal epithelial full-layer detachment: an endemic outbreak with uncommon manifestations. Clin Ophthalmol 2015;9:953-957.
19. Sohrabi M, Goodarzi Z, Saberfar E, Lashini H. The prevalence of viral conjunctivitis in patients who referred to eye specialist hospitals in Tehran, Iran. Iran J Ophthalmol 2014;26:29-32.
20. Akiyoshi K, Suga T, Fukui K, Taniguchi K, Okabe N, Fujimoto T. Outbreak of epidemic keratoconjunctivitis caused by adenovirus type 54 in a nursery school in Kobe City, Japan in 2008. Jpn J Infect Dis 2011;64:353-355.
21. Goudarzi H, Rostami S, Eslami G, Rahbar AS, Miraghasi F, Besharat M, et al. Frequency of adenoviral conjunctivitis by cell culture and PCR method in two referral university hospitals in Tehran. Iran J Clin Infect Dis 2006;1:127-129.
22. Huang G, Yao W, Yu W, Mao L, Sun H, Yao W, et al. Outbreak of epidemic keratoconjunctivitis caused by human adenovirus type 56, China, 2012. PLoS One 2014;9(10):e110781.
23. Kaneko H, Suzutani T, Aoki K, Kitaichi N, Ishida S, Ishiko H, et al. Epidemiological and virological features of epidemic keratoconjunctivitis due to new human adenovirus type 54 in Japan. Br J Ophthalmol 2011;95:32-36.
24. Ishiko H, Shimada Y, Konno T, Hayashi A, Ohguchi T, Tagawa Y, et al. Novel human adenovirus causing nosocomial epidemic keratoconjunctivitis. J Clin Microbiol 2008;46:2002-2008.
25. Kaneko H, Ishiko H, Ohguchi T, Tagawa Y, Aoki K, Suzutani T, et al. Nucleotide sequence variation in the hexon gene of human adenovirus type 8 and 37 strains from epidemic keratoconjunctivitis patients in Japan. J Gen Virol 2009;90:2260-2265.
26. Nakamura M, Hirano E, Kowada K, Ishiguro F, Yamagishi Z, Adhikary AK, et al. Surveillance of adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 1995–2010. J Med Virol 2012;84:81-86.
27. Centers for Disease Control and Prevention. Adenovirus-associated epidemic keratoconjunctivitis outbreaks - four states, 2008-2010. Morb Mortal Wkly Rep 2013;62:637-641.
28. Yağci R, Akçali A, Yağci S, Konno T, Ishiko H, Duman S, et al. Molecular identification of adenoviral conjunctivitis in Turkey. Eur J Ophthalmol 2010;20:669-674.
29. Jin X-H, Ishiko H, Ha NT, Ohguchi T, Akanuma M, Aoki K, et al. Molecular epidemiology of adenoviral conjunctivitis in Hanoi, Vietnam. Am J Ophthalmol 2006;142:1064-1066.
30. Aoki K, Kato M, Ohtsuka H, Ishii K, Nakazono N, Sawada H. Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus types 4 and 19 infections. Br J Ophthalmol 1982;66:776-780.
31. Ariga T, Shimada Y, Ohgami K, Tagawa Y, Ishiko H, Aoki K, et al. New genome type of adenovirus serotype 4 caused nosocomial infections associated with epidemic conjunctivitis in Japan. J Clin Microbiol 2004;42:3644-3648.
32. Saitoh-Inagawa W, Oshima A, Aoki K, Itoh N, Isobe K, Uchio E, et al. Rapid diagnosis of adenoviral conjunctivitis by PCR and restriction fragment length polymorphism analysis. J Clin Microbiol 1996;34:2113-2116.
33. Tabbara KF, Omar N, Hammouda E, Akanuma M, Ohguchi T, Ariga T, et al. Molecular epidemiology of adenoviral keratoconjunctivitis in Saudi Arabia. Mol Vis 2010; 16: 2132-2136.
34. Ishii K, Nakazono N, Fujinaga K, Fujii S, Kato M, Ohtsuka H, et al. Comparative studies on aetiology and epidemiology of viral conjunctivitis in three countries of East Asia—Japan, Taiwan and South Korea. Int J Epidemiol 1987;16:98-103.
35. Massey J, Henry R, Minnich L, Lamson DM, St George K. Notes from the Field. Health care–associated outbreak of epidemic keratoconjunctivitis — west Virginia, 2015. MMWR Morb Mortal Wkly Rep 2016;65:382-383.
36. Rutala WA, Peacock JE, Gergen MF, Sobsey MD, Weber DJ. Efficacy of hospital germicides against adenovirus 8, a common cause of epidemic keratoconjunctivitis in health care facilities. Antimicrob Agents Chemother 2006;50:1419-1424.
37. Shukla D, Kumar A, Srivastava S, Dhole TN. Molecular identification and phylogenetic study of coxsackievirus A24 variant isolated from an outbreak of acute hemorrhagic conjunctivitis in India in 2010. Arch Virol 2013;158:679-684.
38. Lévêque N, Huguet P, Norder H, Chomel JJ. Enteroviruses responsible for acute hemorrhagic conjunctivitis.Med Mal Infect 2010;40:212-218.
| Files | ||
| Issue | Vol 11 No 2 (2019) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v11i2.1074 | |
| Keywords | ||
| Human adenovirus Polymerase chain reaction Keratoconjunctivitis Serotype | ||
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How to Cite
1.
Shafiei K, Makvandi M, Teimoori A, Samarbafzadeh A, Khataminia G, Jalilian S, Neisi N, Makvandi K, Sadeghi Haj M. Frequency of adenovirus serotype 8 in patients with Keratoconjunctivitis, in Ahvaz, Iran. Iran J Microbiol. 2019;11(2):129-136.
