Antibacterial activity of self-adhesive resin cements against Streptococcus mutans at different time intervals
,
Abstract
Background and Objectives: Self-adhesive resin cements release fluoride and have cytotoxic and preventive monomers against the bacteria in their composition. They have acidic property before their complete setting too. The antibacterial activity of three different self-adhesive resin cements against Streptococcus mutans at different time intervals was investigated in this study.
Materials and Methods: The modified direct contact test was used to evaluate the antibacterial effect of Max-Cem, G-Cem and Bis-Cem on S. mutans after aging the samples in phosphate-buffered saline solution for one hour, 24 hours and 1 week. Data were analyzed using one-way ANOVA, repeated measurement ANOVA and Tukey HSD tests (P<0.05).
Results: The differences in the mean bacterial counts between all the study groups and between the study groups and the corresponding control groups were significant at 1-hour and 24-hour intervals (P<0.001). At 1-week, only the differences between Bis-Cem and G-Cem, between Max-Cem and Bis-Cem, and between Bis-Cem and the corresponding control group were significant (P<0.001). There were significant differences between G-Cem and Max-Cem at all the time intervals (P<0.001). In addition, with the use of Bis-Cem there were significant differences between 1-hour and 1-week (P=0.01) and 24-hour and 1-week (P<0.001).
Conclusion: All the cements exhibited antibacterial activity after 1 hour and 24 hours. However, after 1 week, only Bis-Cem retained its antibacterial activity.
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12. Lewinstein I, Matalon S, Slutzkey S, Weiss EI. Antibacterial properties of aged dental cements evaluated by direct-contact and agar diffusion tests. J Prosthet Dent 2005; 93: 364-371.
13. Weiss EI, Shalhav M, Fuss Z. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Endod Dent Traumatol 1996; 12: 179-184.
14. Magalhaes AP, Moreira FC, Alves DR, Estrela CR, Estrela C, Carriao MS, et al. Silver nanoparticles in resin luting cements: Antibacterial and physiochemical properties. J Clin Exp Dent 2016; 8(4): e415-e422.
15. Tay FR, Pashley DH. Aggressiveness of contemporary self-etching systems. I: Depth of penetration beyond dentin smear layers. Dent Mater 2001; 17: 296-308.
16. Piwowarczyk A, Bender R, Ottl P, Lauer HC. Long-term bond between dual-polymerizing cementing agents and human hard dental tissue. Dent Mater 2007; 23: 211-217.
17. Abo-Hamar SE, Hiller KA, Jung H, Federlin M, Friedl KH, Schmalz G. Bond strength of a new universal self-adhesive resin luting cement to dentin and enamel. Clin Oral Investig 2005; 9: 161-167.
18. Zicari F, Couthino E, De Munck J, Poitevin A, Scotti R, Naert I, et al. Bonding effectiveness and sealing ability of fiber-post bonding. Dent Mater 2008; 24: 967-977.
19. Wang Y, Spencer P. Continuing etching of an all-in-one adhesive in wet dentin tubules. J Dent Res 2005; 84: 350-354.
20. Carvalho AS, Cury JA. Fluoride release from some dental materials in different solutions. Oper Dent 1999; 24: 14-19.
21. Attar N, Turgut MD. Fluoride release and uptake capacities of fluoride-releasing restorative materials. Oper Dent 2003; 28: 395-402.
22. Mukai Y, ten Cate JM. Remineralization of advanced root dentin lesions in vitro. Caries Res 2002; 36: 275-280.
23. Levy SM. An update on fluorides and fluorosis. J Can Dent Assoc 2003; 69: 286-291.
24. Tenuta LM, Zamataro CB, Del Bel Cury AA, Tabchoury CP, Cury JA. Mechanism of fluoride dentifrice effect on enamel demineralization. Caries Res 2009; 43: 278-285.
25. Van Dijken J, Kalfas S, Litra V, Oliveby A. Fluoride and mutans streptococci levels in plaque on aged restorations of resin-modified glass lonomer cement, compomer and resin composite. Caries Res 1997; 31: 379-383.
26. Hara AT, Queiroz CS, Freitas PM, Giannini M, Serra MC, Cury JA. Fluoride release and secondary caries inhibition by adhesive systems on root dentine. Eur J Oral Sci 2005; 113: 245-250.
27. Burke FM, Ray NJ, McConnell RJ. Fluoride-containing restorative materials. Int Dent J 2006; 56: 33-43.
28. Yoda A, Nikaido T, Ikeda M, Sonoda H, Foxton RM, Tagami J. Effect of curing method and storage condition on fluoride ion release from a fluoride-releasing resin cement. Dent Mater J 2006; 25: 261-266.
29. Wiegand A, Buchalla W, Attin T. Review on fluoride-releasing restorative materials--fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater 2007; 23: 343-362.
30. Itota T, Okamoto M, Sato K, Nakabo S, Nagamine M, Torii Y, et al. Release and recharge of fluoride by restorative materials. Dent Mater J 1999; 18: 347-353.
31. de Araujo FB, Garcia-Godoy F, Cury JA, Conceicao EN. Fluoride release from fluoride-containing materials. Oper Dent 1996; 21: 185-190.
32. Li L. The biochemistry and physiology of metallic fluoride: action, mechanism, and implications. Crit Rev Oral Biol Med 2003; 14: 100-114.
33. Svensater G, Sjogreen B, Hamilton IR. Multiple stress responses in Streptococcus mutans and the induction of general and stress-specific proteins. Microbiology 2000; 146: 107-117.
2. Villat C, Tran XV, Pradelle-Plasse N, Ponthiaux P, Wenger F, Grosgogeat B, et al. Impedance methodology: A new way to characterize the setting reaction of dental cements. Dent Mater 2010; 26: 1127-1132.
3. Korkmaz FM, Tuzuner T, Baygin O, Buruk CK, Durkan R, Bagis B. Antibacterial activity, surface roughness, flexural strength, and solubility of conventional luting cements containing chlorhexidine diacetate/cetrimide mixtures. J Prosthet Dent 2013; 110: 107-115.
4. Feroz S, Bhoyar A, Khan S. Comparative evaluation of antibacterial effect of dental luting cements on Streptococcus mutans and Lactobacillus acidophilus: An in vitro study. J Contemp Dent Pract 2016; 17: 973-977.
5. Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod 2009; 35: 1051-1055.
6. Han L, Okamoto A, Fukushima M, Okiji T. Evaluation of physical properties and surface degradation of self-adhesive resin cements. Dent Mater J 2007; 26: 906-914.
7. Koo H, Sheng J, Nguyen PT, Marquis RE. Co-operative inhibition by fluoride and zinc of glucosyl transferase production and polysaccharide synthesis by mutans streptococci in suspension cultures and biofilms. FEMS Microbiol Lett 2006; 254: 134-140.
8. Runnacles P, Correr GM, Baratto Filho F, Gonzaga CC, Furuse AY. Degree of conversion of a resin cement light-cured through ceramic veneers of different thicknesses and types. Braz Dent J 2014; 25: 38-42.
9. Aguiar TR, Pinto CF, Cavalli V, Nobre-dos-Santos M, Ambrosano GM, Mathias P, et al. Influence of the curing mode on fluoride ion release of self-adhesive resin luting cements in water or during pH-cycling regimen. Oper Dent 2012; 37: 63-70.
10. Ebrahimi Chaharom ME, Ajami AA, Abed Kahnamouei M, Jafari Navimipour E, Tehranchi P, Zand V, et al. Antibacterial effect of all-in-one self-etch adhesives on Enterococcus faecalis. J Dent Res Dent Clin Dent Prospects 2014; 8: 225-229.
11. Huang Q, Huang S, Liang X, Qin W, Liu F, Lin Z, et al. The antibacterial, cytotoxic, and flexural properties of a composite resin containing a quaternary ammonium monomer. J Prosthet Dent 2018; 120: 609-616.
12. Lewinstein I, Matalon S, Slutzkey S, Weiss EI. Antibacterial properties of aged dental cements evaluated by direct-contact and agar diffusion tests. J Prosthet Dent 2005; 93: 364-371.
13. Weiss EI, Shalhav M, Fuss Z. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Endod Dent Traumatol 1996; 12: 179-184.
14. Magalhaes AP, Moreira FC, Alves DR, Estrela CR, Estrela C, Carriao MS, et al. Silver nanoparticles in resin luting cements: Antibacterial and physiochemical properties. J Clin Exp Dent 2016; 8(4): e415-e422.
15. Tay FR, Pashley DH. Aggressiveness of contemporary self-etching systems. I: Depth of penetration beyond dentin smear layers. Dent Mater 2001; 17: 296-308.
16. Piwowarczyk A, Bender R, Ottl P, Lauer HC. Long-term bond between dual-polymerizing cementing agents and human hard dental tissue. Dent Mater 2007; 23: 211-217.
17. Abo-Hamar SE, Hiller KA, Jung H, Federlin M, Friedl KH, Schmalz G. Bond strength of a new universal self-adhesive resin luting cement to dentin and enamel. Clin Oral Investig 2005; 9: 161-167.
18. Zicari F, Couthino E, De Munck J, Poitevin A, Scotti R, Naert I, et al. Bonding effectiveness and sealing ability of fiber-post bonding. Dent Mater 2008; 24: 967-977.
19. Wang Y, Spencer P. Continuing etching of an all-in-one adhesive in wet dentin tubules. J Dent Res 2005; 84: 350-354.
20. Carvalho AS, Cury JA. Fluoride release from some dental materials in different solutions. Oper Dent 1999; 24: 14-19.
21. Attar N, Turgut MD. Fluoride release and uptake capacities of fluoride-releasing restorative materials. Oper Dent 2003; 28: 395-402.
22. Mukai Y, ten Cate JM. Remineralization of advanced root dentin lesions in vitro. Caries Res 2002; 36: 275-280.
23. Levy SM. An update on fluorides and fluorosis. J Can Dent Assoc 2003; 69: 286-291.
24. Tenuta LM, Zamataro CB, Del Bel Cury AA, Tabchoury CP, Cury JA. Mechanism of fluoride dentifrice effect on enamel demineralization. Caries Res 2009; 43: 278-285.
25. Van Dijken J, Kalfas S, Litra V, Oliveby A. Fluoride and mutans streptococci levels in plaque on aged restorations of resin-modified glass lonomer cement, compomer and resin composite. Caries Res 1997; 31: 379-383.
26. Hara AT, Queiroz CS, Freitas PM, Giannini M, Serra MC, Cury JA. Fluoride release and secondary caries inhibition by adhesive systems on root dentine. Eur J Oral Sci 2005; 113: 245-250.
27. Burke FM, Ray NJ, McConnell RJ. Fluoride-containing restorative materials. Int Dent J 2006; 56: 33-43.
28. Yoda A, Nikaido T, Ikeda M, Sonoda H, Foxton RM, Tagami J. Effect of curing method and storage condition on fluoride ion release from a fluoride-releasing resin cement. Dent Mater J 2006; 25: 261-266.
29. Wiegand A, Buchalla W, Attin T. Review on fluoride-releasing restorative materials--fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater 2007; 23: 343-362.
30. Itota T, Okamoto M, Sato K, Nakabo S, Nagamine M, Torii Y, et al. Release and recharge of fluoride by restorative materials. Dent Mater J 1999; 18: 347-353.
31. de Araujo FB, Garcia-Godoy F, Cury JA, Conceicao EN. Fluoride release from fluoride-containing materials. Oper Dent 1996; 21: 185-190.
32. Li L. The biochemistry and physiology of metallic fluoride: action, mechanism, and implications. Crit Rev Oral Biol Med 2003; 14: 100-114.
33. Svensater G, Sjogreen B, Hamilton IR. Multiple stress responses in Streptococcus mutans and the induction of general and stress-specific proteins. Microbiology 2000; 146: 107-117.
| Files | ||
| Issue | Vol 11 No 4 (2019) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijm.v11i4.1469 | |
| Keywords | ||
| Anti-bacterial agents Resin cements Self-etch primer Dental caries Bacteria | ||
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How to Cite
1.
Ajami A-A, Rikhtegaran S, Bahari M, Hamadanchi S. Antibacterial activity of self-adhesive resin cements against Streptococcus mutans at different time intervals. Iran J Microbiol. 2019;11(4):313-319.
