Zinc solubilization characteristics of efficient siderophore-producing soil bacteria
Background and Objectives: Iron and zinc are two essential micro-nutrients for plant growth and development. Therefore, isolation of siderophores-producing and zinc-solubilizing rhizobacteria involved in bio-availability of these elements is of great interest.
Materials and Methods: In this study, soil samples collected from slightly alkaline soil types were screened for high levels of siderophore secretion and zinc solubilization.
Results: Among positive colonies, three isolates, named F21A, F37 and F38, were able to secrete siderophore at high levels, ranged between 200 and 300 µM/liter. A close association was observed between siderophore production capability and growth rate as an indicator of active metabolism. Siderophore production was closely correlated with the level of zinc ion released into the medium as well. All three siderophore producing isolates were able to withstand temperature as high as 37°C, high concentration of NaCl (up to 2.5%) and a wide range of initial pH from 6 to 9 while hydrolyzing Zn compounds actively. One of the isolates, F21A, tolerated the presence of 200 mgl-1 of zinc. Biochemical and molecular characteristics are indicative that these isolates are Pseudomonas japonica. As experienced in a greenhouse experiment, inoculation with the F21A and F37 isolates significantly increase the plants height, fresh and dry weight of corn with compared to control.
Conclusion: These findings demonstrated that the potential of P. japonica strains as plants growth promoting rhizobacteria (PGPR) in iron and zinc deficient soils.
Desai S, Kumar GP, Sultana U, Pinisetty S, Ahmed SMH, Amalraj ELD, et al. Potential microbial candidate strains for management of nutrient requirements of crops. Afr J Microbiol Res 2012; 6:3924-3931.
Singh JS. Plant growth promoting rhizobacteria. Resonance 2013; 18:275-281.
Omidvari M, Sharifi RA, Ahmadzadeh M, Dahaji PA. Role of fluorescent Pseudomonads siderophore to increase bean growth factors. J Agric Sci 2010; 2:242-247.
Rachid D, Ahmed B. Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens. Afr J Biotechnol 2005; 4:697-702.
Kraemer SM. Iron oxide dissolution and solubility in the presence of siderophores. Aquat Sci 2004; 66:3-18.
Pérez-Miranda S, Cabirol N, George-Téllez R, Zamudio-Rivera L, Fernández FJ. O-CAS, a fast and universal method for siderophore detection. J Microbiol Methods 2007; 70:127-131.
Baakza A, Vala AK, Dave BP, Dube HC. A comparative study of siderophore production by fungi from marine and terrestrial habitats. J Exp Mar Biol Ecol 2004; 311:1-9.
Natheer SE, Muthukkaruppan S. Assessing the in vitro zinc solubilization potential and improving sugarcane growth by inoculating Gluconacetobacter diazotrophicus. Ann Microbiol 2012; 62:435-441.
Rehman H-u, Aziz T, Farooq M, Wakeel A, Rengel Z. Zinc nutrition in rice production systems: a review. Plant soil 2012; 361:203-226.
Saravanan VS, Subramoniam SR, Raj SA. Assessing in vitro solubilization potential of different zinc solubilizing bacterial (zsb) isolates. Braz J Microbiol 2003; 34:121-125.
Singh B, Natesan SKA, Singh B, Usha K. Improving zinc efficiency of cereals under zinc deficiency. Curr Sci 2005; 88:36-44.
Shahab S, Ahmed N. Effect of various parameters on the efficiency of zinc phosphate solubilization by indigenous bacterial isolates. Afr J Biotechnol 2008; 7:1543-1549.
Alexander DB, Zuberer DA. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Fertil Soils 1991; 12:39-45.
Saravanan V, Madhaiyan M, Thangaraju M. Solubilization of zinc compounds by the diazotrophic, plant growth promoting bacterium Gluconacetobacter diazotrophicus. Chemosphere 2007; 66:1794-1798.
Carrillo-Castañeda G, Muñoz JJ, Peralta-Videa JR. A spectrophotometric method to determine the siderophore production by strains of fluorescent Pseudomonas in the presence of copper and iron. Microchem J 2005; 81:35-40.
Dhanya MK, Potty VP. Siderophore production by Pseudomonas fluorescens isolated from the rhizosphere of Solenostemon rotundifolius. J Root Crops 2008; 33:138-140.
Sayyed RZ, Badgujar MD, Sonawane HM, Mhaske MM, Chincholkar SB. Production of microbial iron chelators (siderophores) by fluorescent Pseudomonads. Indian J Biotechnol 2005; 4:484-490.
Rasouli SMH, Malakouti MJ, Khavazi K, Ghanadi MM. The role of fluorescent Pseudomonad's siderophore on Zn absorption in wheat by using 65Zn. J Nucl Sci Technol 2008; 1:20-30.
Palleroni NJ. Pseudomonas. Bergey's Manual of Systematics of Archaea and Bacteria: John Wiley & Sons, Ltd; 2015.
Reid NM, Bowers TH, Lloyd-Jones G. Bacterial community composition of a wastewater treatment system reliant on N2 fixation. Appl Microbiol Biotechnol 2008; 79:285-292.
Barton LL, Abadía J (2006).Iron nutrition in plants and rhizospheric microorganisms. Springer Science & Business Media.
Rajkumar M, Ae N, Prasad MNV, Freitas H. Potential of siderophore-producing bacteria for improving heavy metal phytoextraction. Trends Biotechnol 2010; 28:142-149.
Jefwa JM, Ohiokpehai O, Kavoo AI, Wasike VW. Soil microbe mediated zinc uptake in soy bean: A review. Afr J Food Agric Nutr Dev 2010; 10:4340-4349.
Nosrati R, Owlia P, Saderi H, Rasooli I, Malboobi MA. Phosphate solubilization characteristics of efficient nitrogen fixing soil Azotobacter strains. Iran J Microbiol 2014; 6:285.
Di Simine CD, Sayer JA, Gadd GM. Solubilization of zinc phosphate by a strain of Pseudomonas fluorescens isolated from a forest soil. Biol Fertil Soils 1998; 28:87-94.
Farajzadeh D, Yakhchali B, Aliasgharzad N, Sokhandan-Bashir N, Farajzadeh M. Plant growth promoting characterization of indigenous Azotobacteria isolated from soils in Iran. Curr Microbiol 2012; 64:397-403.
Milagres AMF, Machuca A, Napoleão D. Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. J Microbiol Methods 1999; 37:1-6.
Saha R, Saha N, Donofrio RS, Bestervelt LL. Microbial siderophores: a mini review. J Basic Microbiol 2013; 53:303-317.
Gaonkar T, Bhosle S. Effect of metals on a siderophore producing bacterial isolate and its implications on microbial assisted bioremediation of metal contaminated soils. Chemosphere 2013; 93:1835-1843.
Dave B, Dube H. Regulation of siderophore production by iron Fe (III) in certain fungi and fluorescent Pseudomonads. Indian J Exp Biol 2000; 38:297-299.
Rossbach S, Wilson TL, Kukuk ML, Carty HA. Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens. FEMS Microbiol Lett 2000; 191:61-70.
Iqbal U, Jamil N, Ali I, Hasnain S. Effect of zinc-phosphate-solubilizing bacterial isolates on growth of Vigna radiata. Ann Microbiol 2010; 60:243-248.
Alloway BJ (2008).Zinc in soils and crop nutrition. International Zinc Association Brussels, Belgium and International Fertilizer Industry Association, Paris pp 135.
Sharma SK, Sharma MP, Ramesh A, Joshi OP. Characterization of zinc-solubilizing Bacillus isolates and their potential to influence zinc assimilation in soybean seeds. J Microbiol Biotechnol 2012; 22:352-359.
Vaid SK, Kumar B, Sharma A, Shukla AK, Srivastava PC. Effect of zinc solubilizing bacteria on growth promotion and zn nutrition of rice. J soil sci plant, nut 2014; 14:889-910.
Sah S, Singh N, Singh R. Iron acquisition in maize (Zea mays L.) using Pseudomonas siderophore. 3 Biotech 2017; 7:121.
Sharma A, Johri B. Growth promoting influence of siderophore-producing Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions. Microbiol Res 2003; 158:243-248.
Goteti PK, Emmanuel LDA, Desai S, Shaik MHA. Prospective zinc solubilising bacteria for enhanced nutrient uptake and growth promotion in maize (Zea mays L.). Int J microbiol 2013; Article ID 869697, 7 pages.