Molecular detection of Anaplasma phagocytophilum in carrier cattle of Iran - first documented report
Abstract
Background and Objectives: Anaplasma phagocytophilum is a zoonotic, tick borne rickettsial pathogen. A. phagocytophilum has been detected in North America, Europe, Africa and Asia by molecular methods. In Iran we have little information about the distribution of this agent in human and animals.
Materials and Methods: From March 2007 to July 2007, one hundred and fifty blood samples and corresponding blood smears of cattle without any signs of disease were prepared from a region in Isfahan, Iran with previous history of tick borne disease outbreak.The blood smears were first stained with Giemsa and analyzed for the presence of A. phagocytophilum in the neutrophils. The extracted DNA from blood cells were analyzed by A. phagocytophilum specific nested PCR using primers derived from the 16S rRNA gene.
Results: All blood smears were negative for A. phagocytophilum like structures by Giemsa staining, but 2 out of 150 blood samples (1.33%) were positive for A. phagocytophilum specific nested PCR using specific primers derived from 16S rRNA gene.
Conclusion: This study is the first detection of A. phagocytophilum in carrier cattle in Iran. The present study showed that A. Phagocytophilum is detectable in cattle without any sign of infection but maintained a persistant sub-clinical state in the cattle reservoir, which can be inferred as possible risk for management of public health.
Stuen S. Anaplasma phagocytophilum - the most widespread tick-borne infection in animals in Europe. Vet Res Commun 2007; 31(Suppl. 1): 79–84.
Bakken JS, Dumler JS, Chen S-M, Eckman MR, Van Etta LL, Walker DH. Human granulocytic ehrlichiosis in the upper midwest United States, a new species emerging? J A M A 1994; 272: 212–218.
Chen S-M, Dumler JS, Bakken JS, Walker DH. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human diseases. J Clin Microbiol 1994; 32: 589–595.
Walker DH, Dumler JS. Emergence of the ehrlichioses as human health problems. Emerg Infect Dis 1996; 2: 18–29.
Strle F. Human granulocytic ehrlichiosis in Europe. I J Med Microbiol 2004; 293, S37: 27–35.
Woldehiwet Z, Scott GR (1993). Tick-borne (pasture) fever. In: Woldehiwet Z., Ristic M. (eds.): Rickettsial and chlamydial diseases of domestic animals. Pergamon Press, Oxford, pp. 233-254.
Bashiribod H, Kazemi K, Eslami G, Bigdeli S, Bandehpour M, Rahbarian N, et al. First molecular detection of Anaplasma phagocytophilum in Ixodes ricinus ticks in Iran. J Med S Aci 2004; 4(4): 282-286.
Bown KJ, Begon M, Bennett M, Woldehiwet Z, Ogden NH. Seasonal dynamics of Anaplasma phagocytophila in a rodent–tick (Ixodes trianguliceps) system, United Kingdom. Emerg Infect Dis 2003; 9: 63–70.
Halinska D, Langrova K, Pejcoch M, Pavlasek I. Detection of Anaplasma phagocytophilum in animals by real-time polymerase chain reaction. APMIS 2004; 112:239–247.
Liz JS, Sumner JW, Pfister K, Brossard M. PCR detection and serological evidence of granulocytic ehrlichial infection in roe deer (Capreolus capreolus) and chamois (Rupicapra rupicapra). J Clin Microbiol 2002; 40:892–897.
Bakken JS, Krueth J, Wilson-Nordskog C, Tilden RL, Asanovich K, Dumler JS. Clinical and laboratory characteristics of human granulocytic ehrlichiosis. JAMA 1996; 275: 199–205.
Dumler JS, Choi KS, Garcia-Garcia JC, Barat NS, Scorpio DG, Garyu Grab JW, et al. Human Granulocytic anaplasmosis and Anaplasma phagocytophilum. Perspective 2005; 12:1828-1834.
W o l d e h i w e t Z. Immune evasion and immunosuppression by Anaplasma phagocytophilum, the causative agent of tick-borne fever of ruminants and human granulocytic anaplasmosis, A review. TVJ 2008;175: 37–44.
Noaman V, Shayan P, Amininia N. Molecular diagnostic of Anaplasma marginale in carrier cattle. I J Parasitol 2009; (4(1): 31-38.
Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, et al. Reorganization of genera in the families Rickettsiaceae and Anaplasma taceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and “HGE agent” as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 2001; 51: 2145–2165.
Kocan KM, de la Fuente J, Guglielmone AA, Melendez RD. Antigens and alternatives for control of Anaplasma marginale infection in cattle. Clin Microbiol Rev 2003; 16:698–712.
Rikihisa Y. The tribe Ehrlichieae and ehrlichial diseases. Clin Microbiol Rev 1991; 4:286–308.
Kawahara M, Rikihisa Y, Lin Q, Isogai E, Tahara K, Itagaki A, et al. Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp. in wild deer and ticks on two major islands in Japan. Appl Environ Microbiol 2006; 72:1102–1109.
Park JH, Heo EJ, Choi KS, Dumler JS, Chae JS. Detection of antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis antigens in sera of Korean patients by Western immunoblotting and indirect immunofluorescence assays. Clin Diagn Lab Immunol 2003; 10:1059–1064.
Ooshiro M, Zakimi S, Matsukawa Y, Katagiri Y, Inokuma H. Detection of Anaplasma bovis and Anaplasma phagocytophilum from cattle on Yonaguni Island, Okinawa, Japan. Vet Parasitol 2008; 154: 360–364.
Bekker CP, de Vos A, Taoufik A, Sparagano OA, Jongejan F. Simultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridisation. Vet Microbiol 2002; 89: 223–238.
Lew AE, Gale KR, Minchin CM, Shkap V, de Waal DT. Phylogenic analysis of the erythrocytic Anaplasma species based on 16SrDNA and GroEL (HSP60) sequences of A. marginale, A. centrale and A. ovis and the specific detection of A. centrale vaccine strain. Vet Microbiol 2003;92:145-160.
Molad T, Mazuz ML, Fleiderovitz L, Fish L, Savitsky I, Krigel Y, et al. Molecular and serological detection of A. centrale- and A.marginale-infected cattle grazing within an endemic area. Vet Microbiol 2006; 113:55–62.
Liz JS, Anderes L, Sumner JW, Massung RF, Gern L, Rutti B, et al. PCR detection of granulocytic ehrlichiae in Ixodes ricinus ticks and wild small mammals in western Switzerland. J Clin Microbiol 2000; 38: 1002–1007.
Keysary A, Massung RF, Inbar M, Wallach AD, Shanas U, Mumcuoglu KY, et al. Molecular evidence for Anaplasma phagocytophilum in Israel. Emerg Infect Dis 2007; 13(9):1411-2.
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| Issue | Vol 1 No 2 (2009) | |
| Section | Articles | |
| Keywords | ||
| Anaplasma phagocytophilum 16S rRNA gene nested-PCR carrier cattle Iran | ||
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