Detection of Salmonella spp in commercial eggs in Iran
Abstract
Background and Objective: Salmonellosis can be acquired through consumption of infected raw or undercooked eggs. The aim of this study was isolation and identification of Salmonella spp from the eggshells and the egg contents samples of Tabriz retails.
Methods: A total number of 150 samples of eggs were analyzed for the presence of Salmonella spp. using conventional culture method and multiplex-PCR.
Results: Two (1.33%) out of 150 samples from eggshells were determined as contaminated with Salmonella spp. Salmonella spp was not isolated from the egg contents. Salmonella serovar was determined as enteritidis and typhimurium.
Conclusion: The results of the present study provide the recent dataset of the prevalence of S. enteritidis and S. typhimurium in eggs at retail shops in the northwest of Iran. It is important to remember that control is required at all levels in the food chain and by separating cooked and raw.
Martelli F, Davies RH. Salmonella serovars isolated from table eggs: An overview. Food Res Int 2012;45:745-754.
Uzzau S, Brown DJ, Wallis T, Rubino S, Leori G, Bernard S, et al. Host adapted serotypes of Salmonella enterica. Epidemiol Infect 2000; 125:229-255.
EFSA. Scientific opinion on a quantitative estimate of the public health impact of setting a new target for the reduction of Salmonella in laying hens. EFSA J 2010;8:1546.
Patrick ME, Adcock PM, Gomez TM, Altekruse SF, Holland BH, Tauxe RV, et al. Salmonella enteritidis infections, United States, 1985–1999. Emerg Infect Dis 2004; 10:1.
Gantois I, Ducatelle R, Pasmans F, Haesebrouck F, Gast R, Humphrey TJ, et al. Mechanisms of egg contamination by Salmonella Enteritidis. FEMS Microbiol Rev 2009; 33:718-738.
Downes FP, Ito K. Compendium of methods for the microbiological examination of foods (4th ed.) American Public Health Association, Washington, DC 2001:473-81.
Howard ZR, O’Bryan CA, Crandall PG, Ricke SC.Salmonella Enteritidis in shell eggs: Current issues and prospects for control. Food Res Int 2012; 45:755-764.
Gillespie I, O’Brien S, Adak G, Ward L, Smith H.Foodborne general outbreaks of Salmonella Enteritidis phage type 4 infection, England and Wales, 1992-2002: where are the risks? Epidemiol Infect 2005; 133:795-801.
Crespo PS, Hernández G, Echeíta A, Torres A, Ordóñez P, Aladueña A. Surveillance of foodborne disease outbreaks associated with consumption of eggs and egg products: Spain, 2002–2003. Euro Surveillsnce 2005;10:E050616.
Mirzaie S, Hassanzadeh M, Ashrafi I. Identification and characterization of Salmonella isolates from captured house sparrows. Turk J Vet Anim Sci 2010; 34:181-186.
Jamshidi A, Kalidari G, Hedayati M. Isolation and identification of Salmonella Enteritidis and Salmonella Typhimurium from the eggs of retail stores in Mashhad, Iran using conventional culture method and multiplex PCR assay. J Food Safety 2010; 30:558-568.
Miranzadeh H, Zahraei Salehi T, Karimi V. The count of aerobic mesophill bacteria and isolate Salmonella spp on egg in Isfahan. J Veterinary Journal (Pajouhesh-va- Sazandegi), 2012; 25:31-36.
Mahdavi M, Jalali M, Safaei H, Shamloo E. Microbial quality and prevalence of Salmonella and Listeria in eggs. Int J Env Health Eng 2012; 48:16-21.14. Ghasemian Safaei H, Jalali M, Hosseini A,
Narimani T, Sharifzadeh A, Raheimi E. The prevalence of bacterial contamination of table eggs from retails markets by Salmonella spp., Listeria monocytogenes, Campylobacter jejuni and Escherichia coli in Shahrekord, Iran. Jundishapur J Microb 2012; 4: 249-253.
Betancor L, Pereira M, Martinez A, Giossa G, Fookes M, Flores K, et al. Prevalence of Salmonella enterica in poultry and eggs in Uruguay during an epidemic due to Salmonella enterica serovar Enteritidis. J Clinical Microbiol 2010; 48:2413-2423.
Singh S, Yadav AS, Singh SM, Bharti P. Prevalence of Salmonella in chicken eggs collected from poultry farms and marketing channels and their antimicrobial resistance. Food Res Int 2010; 43:2027-30.
Suresh T, Hatha A, Sreenivasan D, Sangeetha N, Lashmanaperumalsamy P. Prevalence and antimicrobial resistance of Salmonella enteritidis and other Salmonellas in the eggs and egg-storing trays from retails markets of Coimbatore, South India. Food Microbiol 2006; 23:294-299.
Jones D and Musgrove M. Pathogen prevalence and microbial levels associated with restricted shell eggs. J Food Protect 2007; 70:2004-2007.
Jones F, Rives D, Carey J. Salmonella contamination in commercial eggs and an egg production facility. Poultry Sci 1995; 74:753-757.
Chemaly M, Huneau-Salaun A, Labbe A, Houdayer C, Petetin I, Fravalo P. Isolation of Salmonella enterica in laying-hen flocks and assessment of eggshell contamination in France. J Food Protect 2009;72:2071-2077.
Poppe C, Duncan CL, Mazzocco A. Salmonella contamination of hatching and table eggs: a comparison. Can J Vet Res 1998; 62:191-198.
Sasaki Y, Tsujiyama Y, Asai T, Noda Y, Katayama S, Yamada Y. Salmonella prevalence in commercial raw shell eggs in Japan: a survey. Epidemiol Infect 2011;139:1060-1064.
Radkowski M. Occurrence of Salmonella spp. in consumption eggs in Poland. Int J Food Microbiol 2001; 64:189-191.
Zou M, Keelara S, Thakur S. Molecular characterization of Salmonella enterica serotype Enteritidis isolates from humans by antimicrobial resistance, virulence genes, and pulsed-field gel electrophoresis. Foodborne Pathog Dis 2012; 9:232-238.
Moffatt C, Musto J. Salmonella and egg-related outbreaks. Microbiol Aust 2013; 34:94-98.
Files | ||
Issue | Vol 7 No 1 (2015) | |
Section | Original Article(s) | |
Keywords | ||
Egg Salmonella Prevalence Isolation Iran |
Rights and permissions | |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |