Comparison of antifungal activities of various essential oils on the Phytophthora drechsleri, the causal agent of fruit decay
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Abstract
Background and Objectives: The efficacy of Mentha piperita L, Zataria multiflora Boiss and Thymus vulgaris L essential oils (EOs) was evaluated for controlling the growth of Phytophthora drechsleri, the causative agent of damage to many crops that is consumed directly by humans.
Materials and Methods: The EOs used in this study was purchased from Magnolia Co, Iran. The pour plate method in petri dishes containing Potato Dextrose Agar (PDA) was used to evaluate the antifungal properties of EOs. The minimal inhibitory concentrations (MIC), minimum fungicidal concentration (MFC) as well as mycelial growth inhibition (MGI) were measured. The IC50 value (the concentration inhibited 50% of the mycelium growth) was calculated by probit analysis.
Results and Conclusion: The fungal growth was significantly reduced by increasing concentrations of tested EOs. The complete reduction was obtained with Shirazi thyme at all concentrations, whereas the complete reduction for peppermint and thyme was observed at 0.4% and 0.8% (v/v) concentrations, respectively. Meanwhile, the minimum inhibition was observed when 0.1% peppermint (MGI values of 9.37%) was used. The IC50, MIC and MFC values of Shirazi thyme was 0.053, 0.1% and 0.2%, respectively. Similarly, MIC and MFC values of peppermint and thyme were recorded 0.4% and 0.8%, respectively. The results obtained from this study may contribute to the development of new antifungal agents to protect the crops from this pathogenic fungus and many agricultural plant pathogens causing drastic crop losses.
Badawy ME, Rabea EI. A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protec- tion. Int J Carbohydr Chem 2011;2011:1-29.
Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Ni- gro F, Vanadia S, et al. Activity of extracts from wild ed- ible herbs against postharvest fungal diseases of fruit and vegetables. Postharvest Biol Technol 2011;61:72-82.
Al-Reza SM, Rahman A, Ahmed Y, Kang SC. Inhibi- tion of plant pathogens in vitro and in vivo with essen- tial oil and organic extracts of Cestrum nocturnum L. Pestic Biochem Physiol 2010;96:86-92.
Cooke D, Schena L, Cacciola S. Tools to detect, identi- fy and monitor Phytophthora species in natural ecosys- tems. J Plant Pathol 2007;89:13-28.
Alavi A. The role of alternative hosts in overwintering of Phytophthora drechsleri. Appl Entomol Phytopathol 1990;57(1-2):21-2.
Saberi-Riseh R, Hajieghrari B, Rouhani H, Sharifi-Teh- rani A. Effects of inoculum density and substrate type on saprophytic survival of Phytophthora drechsleri, the causal agent of gummosis (crown and root rot) on pis- tachio in Rafsanjan, Iran. Commun Agric Appl Biol Sci 2004;69:653.
Erwin DC, Ribeiro OK. Phytophthora diseases world- wide: American Phytopathological Society (APS Press); 1996.
Mostowfizadeh-ghalamfarsa R, MirsoleimanI Z. Spe- cies-specific identification and detection of Phytoph- thora pistaciae, the causal agent of pistachio gummosis. Phytopathologia Mediterranea. 2013;52:30-45.
Nosrati S. Biocontrol of Phytophthora drechsleri by menthe essential oil in Yazd. Scholarly J Agric Sci 2013;3(3):83-5.
Kreutzer W. A Phytophthora rot of cucumber fruit. Phy- topathology 1937;27:955.
Hardy GSJ, Barrett S, Shearer B. The future of phos- phite as a fungicide to control the soilborne plant patho- gen Phytophthora cinnamomi in natural ecosystems. Australas Plant Pathol 2001;30:133-139.
Cohen Y, Coffey MD. Systemic fungicides and the control of oomycetes. Annu Rev Phytopathol 1986;24(1):311-38.
Tripathi P, Dubey N. Exploitation of natural products as an alternative strategy to control postharvest fungal rot- ting of fruit and vegetables. Postharvest Biol Technol 2004;32:235-245.
Bowers JH, Locke JC. Effect of botanical extracts on the population density of Fusarium oxysporum in soil and control of Fusarium wilt in the greenhouse. Plant Dis 2000;84:300-305.
Ferdeş M, Ungureanu C. Antimicrobial activity of essential oils against four food-borne fungal strains. University Politehnica of Bucharest Scientific Bulletin 2012;74(2).
Askarne L, Talibi I, Boubaker H, Boudyach E, Msanda F, Saadi B, et al. In vitro and in vivo antifungal activity of several Moroccan plants against Penicillium itali- cum, the causal agent of citrus blue mold. Crop Protect 2012;40:53-58.
Mohammadi A, Hashemi M, Hosseini S. Nanoencap- sulation of Zataria multiflora essential oil preparation and characterization with enhanced antifungal activity for controlling Botrytis cinerea, the causal agent of gray mould disease. Innovative Food Science & Emerging Technologies. 2015;28: 73–80.
Hossain MA, Ismail Z, Rahman A, Kang SC. Chemical composition and anti-fungal properties of the essential oils and crude extracts of orthosiphon stamineus Benth. Ind Crops Prod 2008;27:328-334.
Yen T-B, Chang S-T. Synergistic effects of cinnamalde-hyde in combination with eugenol against wood decay fungi. Bioresour Technol 2008;99:232-236.
Costa TR, Fernandes OF, Santos SC, Oliveira Cl, Lião LM, Ferri PH, et al. Antifungal activity of volatile con- stituents of Eugenia dysenterica leaf oil. J Ethnophar- macol 2000;72:111-117.
Kim J, Lee YS, Lee SG, Shin SC, Park IK. Fumigant antifungal activity of plant essential oils and compo- nents from West Indian bay (Pimenta racemosa) and thyme (Thymus vulgaris) oils against two phytopatho- genic fungi. Flavour Fragrance J 2008;23:272-277.
Lahooji A, Mirabolfathy M, karami-osboo R. Effect of Zataria multiflora AND Satureja hortensis essen- tial oils, thymol and carvacrol on growth of Fusarium gramineum isolates and deoxynivalenol production. Iran J Plant Pathol 2010;46:11-13.
Can Baser K. Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Curr Pharm Des 2008;14:3106-3119.
Saei-Dehkordi SS, Tajik H, Moradi M, Khalighi-Siga- roodi F. Chemical composition of essential oils in Za- taria multiflora Boiss. from different parts of Iran and their radical scavenging and antimicrobial activity. Food Chem Toxicol. 2010;48(6):1562-1567.
Gandomi H, Misaghi A, Basti AA, Bokaei S, Khos- ravi A, Abbasifar A, et al. Effect of Zataria multiflora Boiss. essential oil on growth and aflatoxin formation by Aspergillus flavus in culture media and cheese. Food Chem Toxicol 2009;47:2397-2400.
Mahboubi M, Ghazian Bidgoli F. Antistaphylococcal activity of Zataria multiflora essential oil and its syner- gy with vancomycin. Phytomedicine 2010;17:548-550.
Camele I, Altieri L, De Martino L, De Feo V, Mancini E, Rana GL. In vitro control of post-harvest fruit rot fungi by some plant essential oil components. Int J Mol Sci 2012;13:2290-2300.
Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E. Antifungal, phytotoxic and insecticidal prop- erties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresour Technol 2008;99:8788-8795.
Mueller-Riebau F, Berger B, Yegen O. Chemical com- position and fungitoxic properties to phytopathogenic fungi of essential oils of selected aromatic plants grow- ing wild in Turkey. J Agric Food Chem 1995;43:2262-2266.
Soković MD, Vukojević J, Marin PD, Brkić DD, Vajs V, Van Griensven LJ. Chemical Composition of Essen- tial Oilsof Thymus and Mentha Speciesand Their Anti- fungal Activities. Molecules 2009;14:238-249.
Edris AE, Farrag ES. Antifungal activity of peppermint and sweet basil essential oils and their major aroma constituents on some plant pathogenic fungi from the vapor phase. Food/Nahrung 2003;47:117-121.
Soylu EM, Soylu S, Kurt S. Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans. Mycopath- ologia 2006;161:119-128.
Zambonelli A, d’Aulerio AZ, Bianchi A, Albasini A.Effects of Essential Oils on Phytopathogenic Fungi In Vitro*. J Phytopathol 1996;144(9-10):491-494.
Braga P, Dal Sasso M. Morphostructural alterations of Candida albicans induced by thymol. A scanning elec- tron microscopy study. J Chemother 2005;17(supple- ment 3):109.
Taweechaisupapong S, Ngaonee P, Patsuk P, Pitiphat W, Khunkitti W. Antibiofilm activity and post antifun- gal effect of lemongrass oil on clinical Candida dub- liniensis isolate. S Afr J Bot. 2012;78:37-43.
Kroon LP, Brouwer H, de Cock AW, Govers F. The genus Phytophthora anno 2012. Phytopathology 2012;102:348-364.
Abdolmaleki M, Bahraminejad S, Abassi S, Mahmo- di S. Inhibitory effect of some plant extracts onmy- celia growth of Rhizoctonia solani and Phytophtho- ra drechsleri, sugar beet rootrot agent. J Sugar Beet 2010;25:193-205.
Soylu EM, Kurt Ş, Soylu S. In vitro and in vivo anti- fungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cine- rea. Int J Food Microbiol. 2010;143:183-9.
Atti-Santos A, Pansera M, Paroul N, Atti-Serafini L, Moyna P. Seasonal variation of essential oil yield and composition of Thymus vulgaris L.(Lamiaceae) from South Brazil. J Essent Oil Res 2004;16:294-295.
Angioni A, Barra A, Coroneo V, Dessi S, Cabras P.Chemical composition, seasonal variability, and anti- fungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. J Agric Food Chem 2006;54:4364-4370.
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| Issue | Vol 7 No 1 (2015) | |
| Section | Original Article(s) | |
| Keywords | ||
| Mentha piperita Zataria multiflora Thymus vulgaris Essential oil Antifungal Phytophthora drechsleri | ||
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