Methicillin resistance and selective genetic determinants of Staphylococcus aureus isolates with bovine mastitis milk origin
-
Zohreh Ahangari
,
Masoud Ghorbanpoor
,
Masoud Reza Seifiabad Shapouri
,
Darioush Gharibi
,
Kiarash Ghazvini
Abstract
Background and Objectives: Staphylococcus aureus is one of the major causes of bovine mastitis, which can be transmitted from animals to humans. Methicillin-resistant S. aureus (MRSA) isolates are more attentive and if not treated promptly, they can cause death. The aim of this study was to determine the prevalence of methicillin resistance and frequency of selected virulence factors of S. aureus isolates with bovine mastitis milk origin in Ahvaz, southwest of Iran.
Materials and Methods: During a two-year period (2014-2015), 75 S. aureus isolates were recovered from referred clinical and sub-clinical bovine mastitis milk samples. The isolates were phenotypically investigated for resistance to cefoxitin by Kirby-Bauer method. DNA were analyzed by PCR for mecA and selected genes that encode the virulence factors.
Results: According to the results, the spa, ebpS, fnb, bbp, clfA, clfB, and cna genes were detected in 98.7, 97.3, 97.3, 86.7, 84, 84 and 65.3% of the isolates, respectively. Among the 75 isolates, only one (1.3%) isolate was methicillin-resistant. Totally, 39 isolates (50.7%) had all of these virulence factors except mecA. The results showed that 96% of the isolates had at least the fnb, ebpS and spa genes, signifying the noteworthy role of these genes in the pathogenesis of S. aureus bovine intra-mammary infection in this area.
Conclusion: In the present study, the prevalence of mecA was relatively low, possibly indicating that cows do not play a significant role in community-acquired MRSA infection in this area. According to the results, studied virulence factors were somewhat prevalent, bearing in mind the probable risk of transmission of these isolates from cows to humans, especially those that are in close contact with infected cattle. The data presented here can be used for the introduction of a protective vaccine against this infection.
Foster TJ, Geoghegan J, Ganesh VK and Hook M. Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nat Rev Microbiol 2014;12:49-62.
Bradley AJ. Bovine Mastitis: An evolving disease. Vet J 2002;164:116-128.
Peton V, Le Loir Y. Staphylococcus aureus in veterinary medicine. Infect Genet Evol 2014; 21:602-615.
Chambers HF. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev 1997;10,781-791.
Davis MF, Iverson SA, Baron P, Vasse A, Silbergeld EK, Lautenbach, E, et al. Household transmission of meticillin-resistant Staphylococcus aureus and other staphylococci. Lancet Infect Dis 2012;12:703-716.
Baptiste KE, Williams K, Williams NJ, Wattret A, Clegg PD, Dawson, S. et al. Methicillin-resistant staphylococci in companion animals. Emerg Infect Dis 2005;11:1942-1944.
Rybak M, Laplante K. Community-associated methicillin-resistant Staphylococcus aureus: A review. Pharmacotherapy 2005;25:74-85.
Thompson-Crispi K, Atalla H, Miglior F, Mallard BA. Bovine mastitis: Frontiers in immunogenetics. Front Immunol 2014;5:493.
Hu C, Gong R, Guo A, Chen H. Protective effect of ligand-binding domain of fibronectin-binding protein on mastitis induced by Staphylococcus aureus in mice. Vaccine 2010;28:4038-4044.
Flock JI. Extracellular-matrix–binding proteins as targets for the prevention of Staphylococcus aureus infections. Mol Med Today 1999;5:532-537.
Atshan SS, Nor Shamsudin M, Sekawi Z, Lung LTT, Hamat RA, Karunanidhi A, et al. Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus. J Biomed Biotechnol 2012;1-10.
Zong Y, Xu Y, Liang X, Keene DR, Höök A, Gurusiddappa S, et al. A ‘collagen hug' model for Staphylococcus aureus CNA binding to collagen. EMBO J 2005;24:4224-4236.
Bi C, Dong X, Zhong X, Cai H, Wang D, Wang L. Acacetin protects mice from Staphylococcus aureus bloodstream infection by inhibiting the activity of sortase A. Molecules 2016;21:1285.
Sutra L, Poutrel B. Virulence factors involved in the pathogenesis of bovine intramammary infections due to Staphylococcus aureus. J Med Microbiol 1994;40:79-89.
Markey B, Leonard F, Archambault M, Cullinane A, Maguire D (2013). Clinical Veterinary Microbiology. 2nd ed. Mosby Elsevier. Edinburgh London.
Marcos JM, Soriano AC, Salazar MS, Moral CH, Ramos SS, Smeltzer MS, et al. Rapid identification and typing of Staphylococcus aureus by PCR-restriction fragment length polymorphism analysis of the aroA gene. J Clin Microbiol 1999;37:570-574.
Sauer P, Síla J, Štosová T, Večeřová R, Hejnar P, Vágnerová I, et al. Prevalence of genes encoding extracellular virulence factors among meticillin-resistant Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic. J Med Microbiol 2008;57:403-410.
Clinical and Laboratory Standards Institute (2015) Performance standards for antimicrobial susceptibility testing; Twenty-fifth informational supplement, Document M100- S25. CLSI, Wayne, PA.
Ingham KC, Brew S, Vaz D, Sauder DN, McGavin MJ. Interaction of Staphylococcus aureus fibronectin-binding protein with fibronectin affinity, stoichiometry, and modular requirements. J Biol Chem 2004;279:42945-42953.
Paniagua-Contreras G, Sáinz-Espuñes T, Monroy-Pérez E, Raymundo Rodríguez-Moctezuma J, Arenas-Aranda D, Negrete-Abascal E, et al. Virulence markers in Staphylococcus aureus strains isolated from hemodialysis catheters of Mexican patients. Advances Microbiol 2012;2:476-487.
Ghasemian A, Najar Peerayeh S, Bakhshi B, Mirzaee M. The microbial surface components recognizing adhesive matrix molecules (mscramms genes among clinical isolates of Staphylococcus aureus from hospitalized children. Iranian J Pathol 2015;10:258.
Ster C, Gilbert FB, Cochard T, Poutrel B. Transcriptional profiles of regulatory and virulence factors of Staphylococcus aureus of bovine origin: oxygen impact and strain-to-strain variations. Mol Cell Probe 2005;19:227-235.
Merlino J, Watson J, Rose, B, Beard-Pegler M, Gottlieb T, Bradbury R, et al. Detection and expression of methicillin/oxacillin resistance in multidrug-resistant and nonmultidrug-resistant Staphylococcus aureus in central Sydney. J Antimicrob Chemoth 2002;49:793-801.
Juhász-Kaszanyitzky É, Jánosi S, Somogyi P, Dán Á, van Bloois LV, van Duijkeren E, et al. MRSA transmission between cows and humans. Emerg Infect Dis 2007;13(4):630-632.
Sakwinska O, Giddey M, Moreillon M, Morisset D, Waldvogel A, Moreillon P. Staphylococcus aureus host range and human-bovine host shift. Appl Environ Microb 2011;77(17):5908-5915.
Virgin JE, Van Slyke TM, Lombard JE, Zadoks RN. Methicillin-resistant Staphylococcus aureus detection in US bulk tank milk. J Dairy Sci 2009;92:4988-4991.
Haran KP, Godden SM, Boxrud D, Jawahir S, Bender JB, Sreevatsan S. Prevalence and characterization of Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus, isolated from bulk tank milk from Minnesota dairy farms. J Clinl Microbiol 2012;50:688-695.
Hata E, Katsuda K, Kobayashi H, Uchida I, Tanaka K, Eguchi M. Genetic variation among Staphylococcus aureus strains from bovine milk and their relevance to methicillin-resistant isolates from humans. J Clin Microbiol 2010;48:2130-2139.
Kumar R, Yadav BR, Singh RS. Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle. J Biosci 2011;36:175-188.
Havaei SA, Assadbeigi B, Esfahani BN, Hoseini NS, Rezaei N, Havaei SR. Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains. Iran J Microbiol 2015;7:161-167.
Khan A, Javed MT, Mahmood F, Hussain R. Molecular analysis of virulent genes (coa and spa) of Staphylococcus aureus involved in natural cases of bovine mastitis. Pakistan J Agri Sci 2013;50: 739-743.
Pereira UP, Oliveira DG, Mesquita LR, Costa GM, Pereira LJ. Efficacy of Staphylococcus aureus vaccines for bovine mastitis: A systematic review. Vet Microbiol 2011;148:117-124.
Momtaz H, Rahimi E, Tajbakhsh E. Detection of some virulence factors in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran. African J Biotechnol 2010;9:3753-3758.
Saei HD, Aghdasi S, Mohammad-Zadeh H. Frequency analysis of adhesin genes cna, fnbA and fnbB in Staphylococcus aureus isolates recovered from sheep mastitis. Iranian Vet J 2013;9:19-29.
Klein RC, Fabres-Klein MH, Brito MA, Fietto LG, Ribon Ade O. Staphylococcus aureus of bovine origin: Genetic diversity, prevalence and the expression of adhesin-encoding genes. Vet Microbiol 2012;160:183-188.
Gogoi-Tiwari J, Waryah CB, Eto KY, Tau M, Wells K, et al. Relative distribution of virulence-associated factors among Australian bovine Staphylococcus aureus isolates: Potential relevance to development of an effective bovine mastitis vaccine. Virulence 2015;6:419-423.
Ikawaty R, Brouwer EC, Van Duijkeren E, Mevius D, Verhoef J, Fluit AC. Virulence factors of genotyped bovine mastitis Staphylococcus aureus isolates in the Netherlands. Int J Dairy Sci 2010;5:60-70.
Kot B, Szweda P, Frankowska-Maciejewska A, Piechota M, Wolska K. Virulence gene profiles in Staphylococcus aureus isolated from cows with subclinical mastitis in eastern Poland. J Dairy Res 2016;83:228-235.
Tung H S, Guss B, Hellman U, Persson L, Rubin K, and Rydén C. A bone sialoprotein-binding protein from Staphylococcus aureus: a member of the staphylococcal Sdr family. Biochem J 2000;345:611-619.
Puacz E, Ilczyszyn WM, Kosecka M, Buda A, Dudziak W, Polakowska K, et al. Clustering of Staphylococcus aureus bovine mastitis strains from regions of Central-Eastern Poland based on their biochemical and genetic characteristics. Polish J Vet Sci 2015;18:333-342.
Rhem M, Lech E, Patti J, McDevitt D, Hook M, Jones D, Wilhelmus K. The collagen-binding adhesin is a virulence factor in Staphylococcus aureus keratitis. Infect Immun 2000;68:3776-3779.
Elasri MO, Thomas JR, Skinner RA, Blevins JS, Beenken KE, Nelson CL, et al. Staphylococcus aureus collagen adhesion contributes to the pathogenesis of osteomyelitis. Bone 2002;30:275-280.
Coelho SMO, Reinoso E, Pereira IA, Soares LC, Demo M, Bogni C, et al. Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis in Rio de Janeiro. Pesq Vet Bras 2009;29:369-374.
| Files | ||
| Issue | Vol 9 No 3 (2017) | |
| Section | Original Article(s) | |
| Keywords | ||
| Staphylococcus aureus Virulence factor Methicillin resistance Bovine mastitis | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
