Study on mycoflora of poultry feed ingredients and finished feed in Iran
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Abstract
Background and Objectives: Unhygienic poultry feedstuffs can lead to nutrient losses and detrimental effect on poultry production and public health. In the present study, mycobiota and colony-forming units per gram in ingredients and finish poultry feed was evaluated with special reference to potentially mycotoxigenic fungi.
Materials and Methods: Eighty five samples of corn, soybean meal and poultry finished feed were collected from nine poultry feed factories located in three provinces i.e. Tehran, Alborz and Qom in Iran from October 2014 to January 2015. Samples were cultured on Sabouraud dextrose agar (SDA), Aspergillus flavus and parasiticus agar (AFPA) and dichloran rosebengal chloramphenicol agar (DRBC) and incubated at 28 ºC for 7-10 days. Purified fungal colonies were identified by a combination of macro- and microscopic morphological criteria. For determining the rate of fungal contamination, samples were cultured on SDA and colony forming units (CFUs) were calculated.
Results: A total of 384 fungal isolates belonging to 7 genera of filamentous fungi and yeasts were obtained from corn (124 isolates), soybean meal (92 isolates), and feed before (72 isolates), and after pelleting (96 isolates). The most prominent fungal isolate in corn, soybean meal and feed before pelleting (feed as mash form) was Fusarium but in feed after pelleting was Aspergillus. Among 5 Aspergillus species isolated, potentially aflatoxigenic A. flavus isolates was predominant in corn (46.6%), soybean meal (72.7%) and poultry finished feed (75%). CFUs results indicated that 9/22 corn samples (40.9%), none of 22 soybean meal samples, 19/41 finished feed (46.3%) were contaminated higher than the standard limit.
Conclusions: Our results indicated that corn, soybean meal and finished feed of poultry feed mill are contaminated with various fungal genera by different levels sometimes higher that the standard limits. Contamination with potentially myco- toxigenic fungi especially Aspergillus species may be considered as a human public health hazard.
Khosravi AR, Dakhili M, Shokri H. A mycological survey on feed ingredients and mixed animal feeds in Ghom province, Iran. Pakistan J Nutr 2008; 7:31-34.
Kpodo K, Thrane U, Hald B. Fusaria and fumonisins in maize from Ghana and their co-occurrence with afla- toxins. Int J Food Microbiol 2000; 61:147-157.
Labuda R, Tanvinova D. Fungi recovered from Slovaki- an poultry feed mixtures and their toxinogenicity. Ann Agric Environ Med 2006; 13:193-200.
Magnoli C, Astoreca A, Chiacchiera SM, Dalcero A.Occurrence of ochratoxin A and ochratoxigenic myco- flora in corn and corn based foods and feeds in some South American countries. Mycopathologia 2007;163:249-260.
Magnoli C, Hallak C, Astoreca A, Ponsone L, Chiac- chiera SM, Palacio G, et al. Surveillance of toxigenic fungi and ochratoxin A in feedstuffs from Córdoba province. Vet Res Commun 2005; 29:431-445.
Alam MS, Islam MR, Banu MS. Aboundance of fungal flora in relation to moisture content and storage period in different types of poultry feed ingredient. Pakistan J Biol Sci 2001; 41:194-198.
Ezekiel CN, Atehnkeng J, Odebode AC, Bandyopad- hyay R. Distribution of aflatoxigenic Aspergillus sec- tion Flavi in commercial poultry feed in Nigeria. Int J Food Microbiol 2014; 189:18–25.
Yiannikouris A, Jouan JP. Mycotoxins in feeds and their fate in animals: A review. Anim Res 2002; 51:81-99.
Berry CL. The pathology of mycotoxins. J Pathol 1998;154:301-311.
Khanafari A, Soudi H, Miraboulfathi M. Biocontrol of Aspergillus flavus and aflatoxin B production in corn. Iranian J Environ Health Sci Eng 2007; 4:163-168.
Magnoli C, Dalcero A, Chiacchiera SM, Miazzo R, Sa´enz M. Enumeration and identification of Aspergil- lus group and Penicillium species in poultry feeds in Argentina. Mycopathologia 1998; 142:27–32.
Smith JE, Solomons G, Lewis C, Anderson JG. The role of mycotoxins in human and animal nutrition and health. Nat Toxins 1995; 3:187-192.
World Health Organization publication. Aflatoxins. International Agency for Research on Cancer (IARC) Summaries & Evaluations 2002; 82:171-179.
Alinezhad S, Tolouee M, Kamalzadeh A, Motalebi AA, Nazeri M, Yasemi M, et al. Mycobiota and afla- toxin B contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Fla- vi. Iranian J Fish Sci 2011; 10:363-374.
Fraga ME, Curvello F, Gatti MJ, Cavaglieri LR, Dalce- ro AM, Rocha-Rasa CA. Potential aflatoxin and ochra- toxin A production by Aspergillus species in poultry feed processing. Vet Res Commun 2007; 31:343-353.
Gibson AM, Baranyi J, Pitt MJ, Eyles MJ, Robert TA.Predicting fungal growth: The effect of water activity on Aspergillus flavus and related species. Int J Food Microbiol 1994; 23:419-431.
Pitt J, Hocking A. Fungi and Food Spoilage, 3rd edi- tion, Springer, Berlin-Germany, 2009.
Ariyo LA, Anthony MH, Lami ML. Survey of myco- toxigenic fungi in concentrated poultry feed in Niger State. Nigeria J Food Res 2013; 2:128-138.
Oliveira GR, Ribeiro JM, Fraga ME, Cavaglieri LR, Direito GM, Keller KM, et al. Mycobiota in poultry feeds and natural occurrence of aflatoxins, fumonisins and zearalenone in the Rio de Janeiro State, Brazil. My- copathologia 2006; 162:355-362.
Accensi F, Abarca ML, Cabanes FJ. Occurrence of As- pergillus species in mixed feeds and component raw materials and their ability to produce ochratoxin A. Food Microbiol 2004; 21:623-627.
Dalcero A, Magnoli C, Luna M, Ancasi G, Reynoso MM, Chiacchiera S, etal. Mycoflora and naturally oc- curring mycotoxins in poultry feeds in Argentina. My- copathologia 1998; 141:37-43.
González HHL, Resnik SL, Boca RT, Marasas WFO.Mycoflora of Argentinian corn harvested in the main production area in 1990. Mycopathologia 1995; 130:29-36.
Rosa CAR, Riberio JMM, Fraga MJ, Gatti M, Cavaglieri LR, Magnoli CE, et al. Mycoflora of poultry feed and ochratoxin-producing ability of isolated As- pergillus and Penicillium species. Vet Microbiol 2006;113:89-96.
Klich MA, Pitt JI. Differentiation of Aspergillus flavus from A. parasiticus and other closely related species. Trans Brit Mycol Soc 1988; 91: 99-108.
Ariyo LA, Anthony MH, Lami ML. Survey of myco- toxigenic fungi in concentrated poultry feed in Niger State. Nigeria J Food Res 2013; 2:128-138.
Astoreca AL, Dalcero AM, Fernandez Pinto V, Vaa-monde G. A survey on distribution and toxigenicity of Aspergillus section Flavi in poultry feeds. Int J Food Microbiol 2011; 146: 38–43.
Atehnkeng J, Ojiambo PS, Donner M, Ikotun T, Sikora RA, Cotty PJ. Distribution and toxigenicity of Asper- gillus species isolated from maize kernels from three agro-ecological zones in Nigeria. Int J Food Microbiol 2008; 122:74-84.
Orsi RB, Corrêa B, Possi CR, Schammass EA, Noguei- ra JR, Dias SMC, Malozzi MAB. Mycoflora and oc- currence of fumonisins in freshly harvested and stored hybrid maize. J Stor Prod Res 2000; 36:75-87.
Bragulat MR, Abarca ML, Castella O, Caban˜es J. My- cological survey on mixed poultry feeds and mixed rabbit feeds. J Sci Food Agric 1995; 67:215-220.
Dalcero A, Magnoli C, Hallak C, Chiacchiera SM, Palacio G, Rosa CDR. Detection of ochratoxin A in animal feeds and capacity to produce this mycotoxin by Aspergillus section Nigri in Argentina. Food Addit Contam 2002; 19:1065-1072.
Khosravi AR, Shokri H, Zaboli F. Grain-Borne My- coflora and Fumonisin B1 From Fresh-Harvested and Stored Rice in Northern Iran. Jundishapur J Microbiol 2013; 6(5):1-6.
Figueroa S, Centeno S, Calvo MA, Rengel A, Adelanta- do C. Mycobiota and concentration of ochratoxin A in concentrated poultry feed from Venezuela. Pak J Biol Sci 2009; 12:589-594.
Okoli IC, Ogbuewu PI, Uchegbu MC, Opara MN;.Oko- rie JO, Omede AA, Okoli GC. Assessment of the my- coflora of commercial poultry feeds sold in the humid tropical environment of Imo State, Nigeria. J Am Sci 2007; 3:5-9.
Baliukoniene V, Bakutis B, Stankevicius H. Mycologi- cal and mycotoxicological evaluation of grain. Ann Ag- ric Environ Med 2003; 10:223-227.
Azarakhsh Y, Sabokbar A, Bayat M. Incidence of the most common toxigenic Aspergillus species in broiler feeds in Kermanshah province, West of Iran. Global Vet 2011; 6:73-77.
Mayahi M, RaziJalali M, Slamat N. Isolation of Asper- gillus and aflatoxin determination in fish meal, corn and soybean meal. Shahidchamran Uni J Sci 2007;17:95-105.
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| Issue | Vol 8 No 1 (2016) | |
| Section | Articles | |
| Keywords | ||
| Mycoflora Aspergillus Poultry feed Mycotoxigenic fungi | ||
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