Molecular detection of Bordetella holmesii in two infants with pertussis-like syndrome: the first report from Iran
-
Masoumeh Nakhost Lotfi
,
Vajiheh Sadat Nikbin
,
Omid Nasiri
,
Farzad Badmasti
,
Fereshteh Shahcheraghi
Abstract
Background and Objectives: Bordetella holmesii is associated with a pertussis-like respiratory syndrome in healthy individuals and also a rare cause of septicaemia, endocarditis, pneumonia, and septic arthritis, mostly in immunocompromised patients. Culture technique and real-time PCR are 2 methods used to detect Bordetella spp.
Materials and Methods: In this study, 435 nasopharyngeal specimens of patients with suspected whooping cough were checked for the presence of B. holmesii using 2 methods of culture technique and real-time PCR.
Results: In this study, we detected hIS1001 and IS481 of B. holmesii in 2 infants suspected of having pertussis-like syndrome.
Conclusion: Our observations demonstrate that accurate diagnosis is needed to discriminate between B. holmesii and B. pertussis infections among pertussis cases; otherwise, it could lead to misestimating pertussis rate and vaccine efficacy.
Weyant RS, Hollis DG, Weaver RE, Amin M, Steigerwalt AG, O'Connor SP, et al. Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia. J Clin Microbiol 1995;33:1-7.
Kamiya H, Otsuka N, Ando Y, Odaira F, Yoshino S, Kawano K, et al. Transmission of Bordetella holmesii during pertussis outbreak, Japan. Emerg Infect Dis 2012;18:1166-1169.
Mooi FR, Bruisten S, Linde I, Reubsaet F, Heuvelman K, van der Lee S, et al. Characterization of Bordetella holmesii isolates from patients with pertussis-like illness in the Netherlands. FEMS Immunol Med Microbiol 2012;64:289-291.
Bouchez V, Guiso N. Bordetella holmesii: comparison of two isolates from blood and a respiratory sample. Adv Infect Dis 2013;3:123-133.
Pittet LF, Emonet S, Schrenzel J, Siegrist C-A, Posfay-Barbe KM. Bordetella holmesii: an under-recognised Bordetella species. Lancet Infect Dis 2014;14:510-519.
Shepard CW, Daneshvar MI, Kaiser RM, Ashford DA, Lonsway D, Patel JB, et al. Bordetella holmesii bacteremia: a newly recognized clinical entity among asplenic patients. Clin Infect Dis 2004;38:799-804.
Yih WK, Silva EA, Ida J, Harrington N, Lett SM, George H. Bordetella holmesii-like organisms isolated from Massachusetts patients with pertussis-like symptoms. Emerg Infect Dis 1999;5:441-443.
Njamkepo E, Bonacorsi S, Debruyne M, Gibaud SA, Guillot S, Guiso N. Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis. J Clin Microbiol 2011;49:4347-4348.
Rodgers L, Martin SW, Cohn A, Budd J, Marcon M, Terranella A, et al. Epidemiologic and laboratory features of a large outbreak of pertussis-like illnesses associated with cocirculating Bordetella holmesii and Bordetella pertussis--Ohio, 2010-2011. Clin Infect Dis 2013:56:322-331.
Dinu S, Guillot S, Dragomirescu CC, Brun D, Lazăr Ş, Vancea G, et al. Whooping cough in South-East Romania: a 1-year study. Diagn Microbiol Infect Dis 2014;78:302-306.
World Health Organization. Department of Immunization, Vaccines and Biologicals, CH-1211 Geneva 27, Switzerland.
Nikbin VS, Shahcheraghi F, Lotfi MN, Zahraei SM, Parzadeh M. Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran. Iran J Microbiol 2013;5:209-214.
Mazengia E, Silva EA, Peppe JA, Timperi R, George H. Recovery of Bordetella holmesii from patients with pertussis-like symptoms: use of pulsed-field gel electrophoresis to characterize circulating strains. J Clin Microbiol 2000;38:2330-2333.
Mattoo S, Cherry JD. Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to Bordetella pertussis and other Bordetella subspecies. Clin Microbiol Rev 2005;18:326-382.
Riffelmann M, Von König CW, Caro V, Guiso N, Group PPC. Nucleic acid amplification tests for diagnosis of Bordetella infections. J Clin Microbiol 2005;43:4925-4929.
Tizolova A, Guiso N, Guillot S. Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome. Eur J Clin Microbiol Infect Dis 2013;32:89-96.
André P, Caro V, Njamkepo E, Wendelboe AM, Van Rie A, Guiso N. Comparison of serological and real-time PCR assays to diagnose Bordetella pertussis infection in 2007. J Clin Microbiol 2008;46:1672-
Tatti KM, Sparks KN, Boney KO, Tondella ML. Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens. J Clin Microbiol 2011;49:4059-4066.
Ghanaie RM, Karimi A, Sadeghi H, Esteghamti A, Falah F, Armin S, et al. Sensitivity and specificity of the world health organization pertussis clinical case definition. Int J Infect Dis 2010;14:e1072-e5.
Zhang X, Weyrich LS, Lavine JS, Karanikas AT, Harvill ET. Lack of cross-protection against Bordetella holmesii after pertussis vaccination. Emerg Infect Dis 2012;18:1771-1779.
Roorda L, Buitenwerf J, Ossewaarde JM, van der Zee A. A real-time PCR assay with improved specificity for detection and discrimination of all clinically relevant Bordetella species by the presence and distribution of three Insertion Sequence elements. BMC Res Notes 2011;4:11.
Grogan JA, Logan C, O’Leary J, Rush R, O’Sullivan N. Real-time PCR-based detection of Bordetella pertussis and Bordetella parapertussis in an Irish paediatric population. J Clin Microbiol 2011;60:722-729.
Pittet LF, Emonet S, François P, Bonetti E-J, Schrenzel J, Hug M, et al. Diagnosis of whooping cough in Switzerland: differentiating Bordetella pertussis from Bordetella holmesii by polymerase chain reaction. PloS one 2014;9.
| Files | ||
| Issue | Vol 9 No 4 (2017) | |
| Section | Original Article(s) | |
| Keywords | ||
| Bordetella holmesii Real-time PCR HIS1001 Pertussis-like Infections | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
