Iranian Journal of Microbiology 2016. 8(5):307-311.

A comparison of antibiotic disks from different sources on Quicolor and Mueller-Hinton agar media in evaluation of antibacterial susceptibility testing
Neda Saffari, Siavosh Salmanzadeh-Ahrabi, Ahya Abdi-Ali, Mohsen Rezaei-Hemami


Background and Objectives: Antibacterial susceptibility testing of clinical bacterial isolates through disk diffusion method plays a major role in antibacterial treatment. One of the main factors affecting the result of these tests is the type, structure and quality of the disks. The main objective of this study was to compare the agreement of antibiotic disks originated from three companies on Quicolor and Mueller-Hinton agar.

Materials and Methods: Quicolor and Mueller-Hinton agar media were used in disk diffusion method. Seventy clinical isolates from Enterobacteriaceae family (21 Klebsiella spp., 36 Escherichia coli, 1 Enterobacter spp. and 12 Shigella spp.) were investigated in the study. After obtaining data, the results were interpreted as resistant, sensitive or intermediate. Kappa coefficient measured the agreement of two media. Coefficient of variation (CV) was also calculated for antibiotic disks.

Results: The kappa agreement values for three types of antibiotic disks on Quicolor and Mueller-Hinton agar plates were good or excellent for all the examined antibiotics. CV values were also very satisfactory in the majority of cases.

Conclusion: Antibiotic disks from three manufacturers can successfully be used on both Quicolor and Mueller-Hinton agar plates.

Keywords: Antibiotic Disks, Disk Diffusion, Quicolor medium


Antibiotic Discs; Disc Diffusion; Quicolor media; Agreement; Kappa statistic; CV

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