Evaluation of antibody responses to outer membrane vesicles (OMVs) and killed whole cell of Vibrio cholerae O1 El Tor in immunized mice

  • Manijeh Sedaghat Department of Microbiology, Pasteur Institute, Tehran, Iran
  • Seyed Davar Siadat Department of Mycobacteriology and Pulmonary Research, Pasteur Institute, Tehran, Iran
  • Esmat Mirabzadeh Department of Biotechnology Research, Pasteur Institute, Tehran, Iran
  • Malihe Keramati Department of Pilot of Nano-Biotechnology, Pasteur Institute, Tehran, Iran
  • Farzam Vaziri Department of Mycobacteriology and Pulmonary Research, Pasteur Institute, Tehran, Iran
  • Morvarid Shafiei Department of Microbiology, Pasteur Institute, Tehran, Iran
  • Fereshteh Shahcheraghi Department of Microbiology, Pasteur Institute, Tehran, Iran
Keywords: Outer membrane vesicle, Vibrio cholerae O1 El Tor, Killed whole cell, Dukoral vaccine


Background and Objectives: Cholera disease remains an important global health problem affecting 3-5 million subjects worldwide. Outer membrane vesicles (OMVs) have been found in a variety of Gram-negative bacteria and act as protective transport vesicles. The aim of this study was to evaluate Immune responses against Vibrio cholerae O1 El Tor clinical strain OMV and compare it with killed whole cell (KWC), complex of (KWC-OMV) as well as the internationally licensed oral cholera vaccine, Dukoral, in serum and intestinal secretions of mice.

Materials and Methods: OMVs were prepared by using modified detergent-centrifugation procedure from V. cholerae O1 El Tor clinical strain from 2005 outbreak. The ultrastructure and content of OMVs were investigated via the Scanning Electron Microscopy (SEM) and SDS-PAGE analysis. Three doses of oral immunization were adjusted and total IgG and IgA in serum and intestinal secretion were measured by enzyme-linked immunosorbent assay (ELISA).

Results: Extracted OMVs from the V. cholerae were spherical vesicles with a size ranging from 10 to 300 nm. OMV-immunized mice showed an increased level of total IgG and IgA both in serum and intestinal secretion when compared to the negative controls. Also, there existed a higher level of secretory IgA than the total IgG, suggesting the most of protection against V. cholerae colonization provided by sIgA.

Conclusion: Our findings revealed that oral immunization with V. cholerae OMVs might induce a long-term immunity, especially when administered in combination with KWC. This study tested the adjuvant activity of OMVs and may be useful in future nano vaccine research.


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How to Cite
Sedaghat M, Siadat SD, Mirabzadeh E, Keramati M, Vaziri F, Shafiei M, Shahcheraghi F. Evaluation of antibody responses to outer membrane vesicles (OMVs) and killed whole cell of Vibrio cholerae O1 El Tor in immunized mice. Iran J Microbiol. 11(3):212-219.
Original Article(s)