Quantitative analysis of Staphylococcus aureus in patients with chronic rhinosinusitis under continuous ultrasound treatment
-
Narjes Feizabadi
,
Javad Sarrafzadeh
,
Mojtaba Fathali
,
Behnoosh Vasaghi-Gharamaleki
,
Mahdi Dadgoo
,
Hossein Kazemian
,
Jalil Kardan-Yamchi
,
Sonia Hesam Shariati
Abstract
Background and Objectives: Bacterial pathogens, in particular drug resistant strains, involved in chronic rhinosinusitis may result in treatment failure. Ultrasound waves are able to destroy bacterial population in sinus cavities and can recover patients.
Materials and Methods: Twelve patients with chronic sinusitis and 10 healthy controls were treated by continuous ultrasound waves. Clinical specimens were collected before and after treatment. Serial diluted specimens were cultured on blood agar, chocolate and MacConkey agar plates for bacterial isolation. Bacterial DNA was extracted and used for Staphylococcus aureus detection using quantitative PCR.
Results: S. aureus was the most isolated bacterium (10 patients), which was eradicated from 8 patients after treatment. Using phenotypic methods at the beginning, 3 out of 10 healthy individuals were found to be positive. From 11 positive patients for S. aureus identified by real time qPCR, 9 showed significant reduction after treatment. In the healthy group, S. aureus was detected in 4 samples using qPCR, but they were clean at the second sampling.
Conclusion: According to our phenotypic and molecular experiments, continuous ultrasound treatment effectively reduced the bacterial population in studied patients (p < 0.01). This was a hopeful basis for doing more studies with ultrasound therapy as a treatment option.
1. Tomassen P, Zele TV, Zhang N, Perez-Novo C, Bruaene NV, Gevaert P, et al. Pathophysiology of chronic rhinosinusitis. Proc Am Thorac Soc 2011; 8:115-120.
2. Timperley D, Schlosser RJ, Harvey RJ. Chronic rhinosinusitis: an education and treatment model. Otolaryngol Head Neck Surg 2010; 143: S3-8.
3. Boase S, Foreman A, Cleland E, Tan L, Melton-Kreft R, Pant H, et al. The microbiome of chronic rhinosinusitis: culture, molecular diagnostics and biofilm detection. BMC Infect Dis 2013; 13: 210.
4. Feazel LM, Robertson CE, Ramakrishnan VR, Frank DN. Microbiome complexity and Staphylococcus aureus in chronic rhinosinusitis. Laryngoscope 2012; 122: 467-472.
5. Suh JD, Cohen NA, Palmer JN. Biofilms in chronic rhinosinusitis. Curr Opin Otolaryngol Head Neck Surg 2010; 18: 27-31.
6. Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 2002; 15: 167-193.
7. Tan NC, Foreman A, Jardeleza C, Douglas R, Tran H, Wormald PJ. The multiplicity of Staphylococcus aureus in chronic rhinosinusitis: correlating surface biofilm and intracellular residence. Laryngoscope 2012; 122: 1655-1660.
8. Abreu NA, Nagalingam NA, Song Y, Roediger FC, Pletcher SD, Goldberg AN. et al. Sinus microbiome diversity depletion and Corynebacterium tuberculostearicum enrichment mediates rhinosinusitis. Sci Transl Med 2012; 4(151): 151ra124.
9. Scherba G, Weigel RM, O'brien WD. Quantitative assessment of the germicidal efficacy of ultrasonic energy. Appl Environ Microbiol 1991; 57: 2079-2084.
10. Qian Z, Sagers RD, Pitt WG. Investigation of the mechanism of the bioacoustic effect. J Biomed Mater Res 1999; 44: 198-205.
11. Rediske AM, Hymas WC, Wilkinson R, Pitt WG. Ultrasonic enhancement of antibiotic action on several species of bacteria. J Gen Appl Microbiol 1998; 44: 283-288.
12. Carmen JC, Runyan CM, Robison RA, Nelson JL, Beckstead BL, Pitt WG, et al. Ultrasonic-enhanced gentamicin transport through colony biofilms of Pseudomonas aeruginosa and Escherichia coli. J Infect Chemother 2004; 10: 193-199.
13. Hedges M, Lewis M, Lunec J, Cramp WA. The Effect of Ultrasound at 1• 5 MHz on Escherichia Coli. Int J Radiat Biol Relat Stud Phys Chem Med 1980; 37: 103-108.
14. Gogate PR, Wilhelm AM, Pandit AB. Some aspects of the design of sonochemical reactors.Ultrason Sonochem 2003; 10: 325-330.
15. Broekman S, Pohlmann O, Beardwood ES, de Meulenaer EC. Ultrasonic treatment for microbiological control of water systems. Ultrasonics Sonochemistry 2010; 17: 1041-1048.
16. Miles AA, Misra SS, Irwin JO. The estimation of the bactericidal power of the blood. J Hyg (Lond) 1938; 38: 732-749.
17. Mahon CR, Lehman DC, Manuselis G. Textbook of diagnostic microbiology-E-Book. Elsevier Health Sciences; 2014 Mar 25.
18. Ghodousi A, Nomanpour B, Davoudi S, Maleknejad P, Omrani M, Kashef N, et al. Application of fnbA gene as new target for the species-specific and quantitative detection of Staphylococcus aureus directly from lower respiratory tract specimens by real time PCR. Indian J Pathol Microbiol 2012; 55: 490-495.
19. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C T method. Nat Protoc 2008; 3:1101-1108.
20. Lee LN, Bhattacharyya N. Regional and specialty variations in the treatment of chronic rhinosinusitis. Laryngoscope 2011; 121:1092-1097.
21. Smith SS, Evans CT, Tan BK, Chandra RK, Smith SB, Kern RC. National burden of antibiotic use for adult rhinosinusitis. J Allergy Clin Immunol 2013; 132: 1230-1232.
22. Bhattacharyya N, Kepnes LJ. Assessment of trends in antimicrobial resistance in chronic rhinosinusitis. Ann Otol Rhinol Laryngol 2008; 117: 448-452.
23. Ghafourian S, Mohebi R, Rezaei M, Raftari M, Sekawi Z, Kazemian H. Comparative analysis of biofilm development among MRSA and MSSA strains. Roum Arch Microbiol Immunol 2012; 71: 175-82.
24. Bordin A, Sidjabat HE, Cottrell K, Cervin A. Chronic rhinosinusitis: a microbiome in dysbiosis and the search for alternative treatment options. Microbiol Aust 2016; 37: 149-152.
25. Bartley J, Ansari NN, Naghdi S. Therapeutic ultrasound as a treatment modality for chronic rhinosinusitis. Curr Infect Dis Rep 2014; 16: 398.
26. Høsøien E, Lund AB, Vasseljen O. Vasseljen, Similar effect of therapeutic ultrasound and antibiotics for acute bacterial rhinosinusitis: a randomised trial. J Physiother 2010; 56: 29-32.
27. Ansari NN, Naghdi S, Farhadi M, Jalaie S. A preliminary study into the effect of low-intensity pulsed ultrasound on chronic maxillary and frontal sinusitis. Physiother Theory Pract 2007; 23: 211-218.
28. Naghdi S, Ansari NN, Farhadi M. A clinical trial on the treatment of chronic rhinosinusitis with continuous ultrasound. J Phys Ther Sci 2008; 20: 233-238.
2. Timperley D, Schlosser RJ, Harvey RJ. Chronic rhinosinusitis: an education and treatment model. Otolaryngol Head Neck Surg 2010; 143: S3-8.
3. Boase S, Foreman A, Cleland E, Tan L, Melton-Kreft R, Pant H, et al. The microbiome of chronic rhinosinusitis: culture, molecular diagnostics and biofilm detection. BMC Infect Dis 2013; 13: 210.
4. Feazel LM, Robertson CE, Ramakrishnan VR, Frank DN. Microbiome complexity and Staphylococcus aureus in chronic rhinosinusitis. Laryngoscope 2012; 122: 467-472.
5. Suh JD, Cohen NA, Palmer JN. Biofilms in chronic rhinosinusitis. Curr Opin Otolaryngol Head Neck Surg 2010; 18: 27-31.
6. Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 2002; 15: 167-193.
7. Tan NC, Foreman A, Jardeleza C, Douglas R, Tran H, Wormald PJ. The multiplicity of Staphylococcus aureus in chronic rhinosinusitis: correlating surface biofilm and intracellular residence. Laryngoscope 2012; 122: 1655-1660.
8. Abreu NA, Nagalingam NA, Song Y, Roediger FC, Pletcher SD, Goldberg AN. et al. Sinus microbiome diversity depletion and Corynebacterium tuberculostearicum enrichment mediates rhinosinusitis. Sci Transl Med 2012; 4(151): 151ra124.
9. Scherba G, Weigel RM, O'brien WD. Quantitative assessment of the germicidal efficacy of ultrasonic energy. Appl Environ Microbiol 1991; 57: 2079-2084.
10. Qian Z, Sagers RD, Pitt WG. Investigation of the mechanism of the bioacoustic effect. J Biomed Mater Res 1999; 44: 198-205.
11. Rediske AM, Hymas WC, Wilkinson R, Pitt WG. Ultrasonic enhancement of antibiotic action on several species of bacteria. J Gen Appl Microbiol 1998; 44: 283-288.
12. Carmen JC, Runyan CM, Robison RA, Nelson JL, Beckstead BL, Pitt WG, et al. Ultrasonic-enhanced gentamicin transport through colony biofilms of Pseudomonas aeruginosa and Escherichia coli. J Infect Chemother 2004; 10: 193-199.
13. Hedges M, Lewis M, Lunec J, Cramp WA. The Effect of Ultrasound at 1• 5 MHz on Escherichia Coli. Int J Radiat Biol Relat Stud Phys Chem Med 1980; 37: 103-108.
14. Gogate PR, Wilhelm AM, Pandit AB. Some aspects of the design of sonochemical reactors.Ultrason Sonochem 2003; 10: 325-330.
15. Broekman S, Pohlmann O, Beardwood ES, de Meulenaer EC. Ultrasonic treatment for microbiological control of water systems. Ultrasonics Sonochemistry 2010; 17: 1041-1048.
16. Miles AA, Misra SS, Irwin JO. The estimation of the bactericidal power of the blood. J Hyg (Lond) 1938; 38: 732-749.
17. Mahon CR, Lehman DC, Manuselis G. Textbook of diagnostic microbiology-E-Book. Elsevier Health Sciences; 2014 Mar 25.
18. Ghodousi A, Nomanpour B, Davoudi S, Maleknejad P, Omrani M, Kashef N, et al. Application of fnbA gene as new target for the species-specific and quantitative detection of Staphylococcus aureus directly from lower respiratory tract specimens by real time PCR. Indian J Pathol Microbiol 2012; 55: 490-495.
19. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C T method. Nat Protoc 2008; 3:1101-1108.
20. Lee LN, Bhattacharyya N. Regional and specialty variations in the treatment of chronic rhinosinusitis. Laryngoscope 2011; 121:1092-1097.
21. Smith SS, Evans CT, Tan BK, Chandra RK, Smith SB, Kern RC. National burden of antibiotic use for adult rhinosinusitis. J Allergy Clin Immunol 2013; 132: 1230-1232.
22. Bhattacharyya N, Kepnes LJ. Assessment of trends in antimicrobial resistance in chronic rhinosinusitis. Ann Otol Rhinol Laryngol 2008; 117: 448-452.
23. Ghafourian S, Mohebi R, Rezaei M, Raftari M, Sekawi Z, Kazemian H. Comparative analysis of biofilm development among MRSA and MSSA strains. Roum Arch Microbiol Immunol 2012; 71: 175-82.
24. Bordin A, Sidjabat HE, Cottrell K, Cervin A. Chronic rhinosinusitis: a microbiome in dysbiosis and the search for alternative treatment options. Microbiol Aust 2016; 37: 149-152.
25. Bartley J, Ansari NN, Naghdi S. Therapeutic ultrasound as a treatment modality for chronic rhinosinusitis. Curr Infect Dis Rep 2014; 16: 398.
26. Høsøien E, Lund AB, Vasseljen O. Vasseljen, Similar effect of therapeutic ultrasound and antibiotics for acute bacterial rhinosinusitis: a randomised trial. J Physiother 2010; 56: 29-32.
27. Ansari NN, Naghdi S, Farhadi M, Jalaie S. A preliminary study into the effect of low-intensity pulsed ultrasound on chronic maxillary and frontal sinusitis. Physiother Theory Pract 2007; 23: 211-218.
28. Naghdi S, Ansari NN, Farhadi M. A clinical trial on the treatment of chronic rhinosinusitis with continuous ultrasound. J Phys Ther Sci 2008; 20: 233-238.
| Files | ||
| Issue | Vol 10 No 6 (2018) | |
| Section | Original Article(s) | |
| Keywords | ||
| Chronic rhinosinusitis Ultrasound treatment Staphylococcus aureus | ||
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How to Cite
1.
Feizabadi N, Sarrafzadeh J, Fathali M, Vasaghi-Gharamaleki B, Dadgoo M, Kazemian H, Kardan-Yamchi J, Hesam Shariati S. Quantitative analysis of Staphylococcus aureus in patients with chronic rhinosinusitis under continuous ultrasound treatment. Iran J Microbiol. 2019;10(6):354-360.
