A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

  • Prabu Siva Sankar Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia
  • Marimuthu Citartan Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia
  • Aminah Ahmed Siti Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia
  • Boris V. Skryabin Faculty of Medicine (TRAM), University of Muenster, Muenster, Germany
  • Timofey S. Rozhdestvensky Faculty of Medicine (TRAM), University of Muenster, Muenster, Germany
  • Goot Heah Khor Centre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buluh Campus, Selangor, Malaysia
  • Thean Hock Tang Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia
Keywords: Protein expression, Pyrococcus furiosus, DNA polymerase, Polymerase chain reaction

Abstract

Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system under a simple purification method.


Materials and Methods: Pfu polymerase gene sequence, derived from Pyrocuccus furiosus (Pfu) genomic DNA, was cloned and overexpressed in E. coli BL21 (DE3) pLysS. Upon overexpression, bacterial lysate containing the Pfu DNA polymerase was heated at 94°C for 5 minutes. Pfu DNA polymerase having high thermal stability was retained while the other bacterial proteins were denatured. The resulting thermo stable Pfu DNA polymerase was separated from the other debris of the denatured proteins by simple centrifugation.


Results: The enzymatic activity of the resulting Pfu DNA polymerase was estimated by comparing with the commercial Pfu DNA Polymerases. An estimated 50000 units of functional Pfu DNA polymerase was produced from a 400 ml culture.


Conclusion: The in-house produced Pfu DNA Polymerase could be used for routine amplification that requires high-fidelity such as cloning and DNA sequencing.

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Published
2019-05-30
How to Cite
1.
Sankar P, Citartan M, Siti A, V. Skryabin B, S. Rozhdestvensky T, Khor G, Tang T. A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification. Iran J Microbiol. 11(2):181-186.
Section
Original Article(s)