A comparison of conventional rapid methods in diagnosis of superficial and cutaneous mycoses based on KOH, Chicago sky blue 6B and calcofluor white stains

  • Parvaneh Afshar Research and Development Unit of Referral Laboratory, Mazandaran University of Medical Sciences, Sari, Iran
  • Lale Vahedi Department of Pathology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Hamed Rouhanizadeh Research and Development Unit of Referral Laboratory, Mazandaran University of Medical Sciences, Sari, Iran; Department of Pediatrics, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Keywords: Rapid diagnosis, Dermatomycosis, Potassium hydroxide, Chicago sky blue 6B, Calcofluor white

Abstract

Background and Objectives: Rapid confirmation of dermatomycoses is desirable, as it allows the clinicians to initiate appropriate therapy immediately. In this study, the utility of a novel contrast stain, Chicago sky blue stain, was compared with potassium hydroxide mount and calcofluor white stain to determine the causative fungal elements in the rapid detection method.Materials and Methods: In this survey, 189 samples of suspected dermatomycosis infections were assessed in 3 incubation times of 30 minutes, 2 hours, and > 6 hours. Results: Positive cases were shown in Chicago sky blue 6B (55%), calcofluor white (53.4%), and potassium hydroxide (36%), with 30-minute incubation. Positive results increased in other incubation times. Sensitivity, specificity, PPV, NPV and accuracy of Chicago sky blue 6B were 97%, 100%, 100%, 96% and 98% and, for potassium hydroxide, they were 66%, 98%, 97%, 98%, 80% versus CFW, respectively.Conclusion: This study provides evidence that the Chicago sky blue 6B stain is a simple, fast and cost-effective method.

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Published
2019-02-10
How to Cite
1.
Afshar P, Vahedi L, Rouhanizadeh H. A comparison of conventional rapid methods in diagnosis of superficial and cutaneous mycoses based on KOH, Chicago sky blue 6B and calcofluor white stains. Iran J Microbiol. 10(6):433-40.
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Original Article(s)