Prevalence, antimicrobial susceptibility, serotyping and virulence genes screening of Listeria monocytogenes strains at a tertiary care hospital in Tehran, Iran
-
Siamak Heidarzadeh
,
Mohammad Mehdi Soltan Dallal
,
Mohammad Reza Pourmand
,
Reihaneh Pirjani
,
Abbas Rahimi Foroushani
,
Matina Noori
,
Aida Babazadeh Naseri
Abstract
Background and Objectives: Listeria monocytogenes is the etiological agent of listeriosis, a highly fatal infection which causes miscarriage or stillbirth in pregnant women. The objective of this study was to detect the prevalence, serotypes, antimicrobial susceptibility and virulence factors of L. monocytogenes isolated from pregnant women with vaginitis at a tertiary care hospital in Tehran, Iran.
Materials and Methods: During September 2015 to February 2017, a total of 400 vaginal swabs were collected from pregnant women. The presumptive isolates were characterized biochemically. All L. monocytogenes isolates were further analyzed by serotyping and antimicrobial susceptibility tests. All positive samples for L. monocytogenes were analyzed for presence of virulence genes (hlyA, actA, inlA, inlC, inlJ and prfA).
Results: Twenty-two (5.5%) of the samples were found positive for presence of L. monocytogenes. Most isolates are resistant to trimethoprim/sulfamethoxazole (81.82%) and chloramphenicol (54.55%). The majority of tested isolates (59.10%) belonged to serotype 4b, followed by 1/2a (22.73%), 1/2b (13.63%), and 3c (4.54%). The hlyA, actA and inlA were detected in all of the 22 L. monocytogenes isolates, but two, three and five isolates were found to lack inlC, inlJ and prfA, respectively. Only one isolate lacked three inlC, inlJ and prfA genes, and two isolates simultaneously lacked both inlJ and prfA genes.
Conclusion: Evaluation of virulence factors and antimicrobial susceptibility can be highly helpful to develop effective treatment strategies against L. monocytogenes infections. This study is noteworthy in that it documents prevalence, virulence characteristics, and antimicrobial resistance of L. monocytogenes.
Chen BY, Pyla R, Kim TJ, Silva JL, Jung YS. Prevalence and contamination patterns of Listeria monocytogenes in catfish processing environment and fresh fillets. Food Microbiol. 2010;27(5):645-52.
Lv J, Qin Z, Xu Y, Xie Q. Listeria infection in Chinese pregnant women and neonates from Shandong. Int J Clin Exp Med. 2014;7(9):2730.
Churchill RL, Lee H, Hall JC. Detection of Listeria monocytogenes and the toxin listeriolysin O in food. J Microbiol Methods. 2006;64(2):141-70.
Liu D, Lawrence ML, Austin FW, Ainsworth AJ. A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes. J Microbiol Methods. 2007;71(2):133-40.
Sant'Ana AS, Igarashi MC, Landgraf M, Destro MT, Franco BD. Prevalence, populations and pheno- and genotypic characteristics of Listeria monocytogenes isolated from ready-to-eat vegetables marketed in Sao Paulo, Brazil. Int J Food Microbiol. 2012;155(1-2):1-9.
Jamali H, Paydar M, Ismail S, Looi CY, Wong WF, Radmehr B, et al. Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets. BMC Microbiol. 2015;15:144.
Rawool DB, Malik SV, Shakuntala I, Sahare AM, Barbuddhe SB. Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases. Int J Food Microbiol. 2007;113(2):201-7.
Nayak DN, Savalia CV, Kalyani IH, Kumar R, Kshirsagar DP. Isolation, identification, and characterization of Listeria spp. from various animal origin foods. Vet World. 2015;8(6):695-701.
Srinivasan V, Nam HM, Nguyen LT, Tamilselvam B, Murinda SE, Oliver SP. Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathog Dis. 2005;2(3):201-11.
Khan JA, Rathore RS, Khan S, Ahmad I. In vitro detection of pathogenic Listeria monocytogenes from food sources by conventional, molecular and cell culture method. Braz J Microbiol. 2013;44(3):751-8.
Okada Y, Monden S, Igimi S, Yamamoto S. The occurrence of Listeria monocytogenes in imported ready-to-eat foods in Japan. J Vet Med Sci. 2012;74(3):373-5.
Ennaji H, Timinouni M, Ennaji MM, Hassar M, Cohen N. Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Morocco. Infect Drug Resist. 2008;1:45-50.
Morvan A, Moubareck C, Leclercq A, Herve-Bazin M, Bremont S, Lecuit M, et al. Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France. Antimicrob Agents Chemother. 2010;54(6):2728-31.
Odjadjare EE, Obi LC, Okoh AI. Municipal wastewater effluents as a source of listerial pathogens in the aquatic milieu of the Eastern Cape Province of South Africa: a concern of public health importance. Int J Environ Res Public Health. 2010;7(5):2376-94.
Norowzi J, Moradi Bidhendy S, Shafiee M. Detection of actA gene in Listeria monocytogenes isolated from dairy products. J Microbiol World. 2013;6(3):246-52.
Carp-Cărare C, Vlad-Sabie A, Floriştean V-C. Detection and serotyping of Listeria monocytogenes in some food products from North-East of Romania. Rev Romana Med Lab. 2013;21(3):285-92.
Borucki MK, Call DR. Listeria monocytogenes serotype identification by PCR. J Clin Microbiol. 2003;41(12):5537-40.
Soni DK, Singh M, Singh DV, Dubey SK. Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples. BMC microbiology. 2014;14(241):1-10.
Bahador A, Kalani BS, Valian F, Irajian G, Lotfollahi L. Phenotypic and Genotypic Characteristics of Listeria monocytogenes Isolated From Dairy and Meat Products. Avicenna J Clin Microbiol Infect. 2015;2(3).
Jamali H, Radmehr B, Thong KL. Prevalence, characterisation, and antimicrobial resistance of Listeria species and Listeria monocytogenes isolates from raw milk in farm bulk tanks. Food Control. 2013;34(1):121-5.
Dehkordi FS, Barati S, Momtaz H, Ahari SNH, Dehkordi SN. Comparison of Shedding, and Antibiotic Resistance Properties of Listeria monocytogenes Isolated From Milk, Feces, Urine, and Vaginal Secretion of Bovine, Ovine, Caprine, Buffalo, and Camel Species in Iran. Jundishapur J Microbiol. 2013;6(3):284-94.
Rahimi E, Momtaz H, Sharifzadeh A, Behzadnia A, Ashtari M, Zandi Esfahani S, et al. Prevalence and antimicrobial resistance of Listeria species isolated from traditional dairy products in Charar Mahal & Bakhtiary, Iran. Bulgarian J Vet Med. 2012;15(2):115-22.
Rawool D, Malik S, Barbuddhe S, Shakuntala I, Aurora R. A multiplex PCR for detection of virulence associated genes in Listeria monocytogenes. Int J Food Saf. 2007;9:56-62.
Osman KM, Zolnikov TR, Samir A, Orabi A. Prevalence, pathogenic capability, virulence genes, biofilm formation, and antibiotic resistance of Listeria in goat and sheep milk confirms need of hygienic milking conditions. Pathog Glob Health. 2014;108(1):21-9.
Liu D, Ainsworth AJ, Austin FW, Lawrence ML. Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genes. J Med Microbiol. 2003;52(Pt 12):1065-70.
Jensen A, Thomsen LE, Jorgensen RL, Larsen MH, Roldgaard BB, Christensen BB, et al. Processing plant persistent strains of Listeria monocytogenes appear to have a lower virulence potential than clinical strains in selected virulence models. Int J Food Microbiol. 2008;123(3):254-61.
Jaradat ZW, Schutze GE, Bhunia AK. Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes. Int J Food Microbiol. 2002;76(1-2):1-10.
Eslami G, Goudarzi H, Ohadi E, Taherpour A, Pourkaveh B, Taheri S. Identification of Listeria monocytogenes Virulence Factors in Women With Abortion by Polymerase Chain Reaction. Arch Clin Infect Dis. 2014;9(3).
Sadeghi Kalani B, Pournajaf A, Sedighi M, Bahador A, Irajian G, Valian F. Genotypic characterization, invasion index and antimicrobial resistance pattern in Listeria monocytogenes strains isolated from clinical samples. J Acut Dis. 2015;4(2):141-6.
Jahangirsisakht A, Kargar M, Mirzaee A, Akbartabar Toori M, Aramesh S, Mohamadkhani N, et al. Assessing Listeria monocytogenes hly A gene in pregnant women with spontaneous abortion using PCR method in Yasuj, south west of Iran. Afr J Microbiol Res. 2013;7(33):4257–60.
Stepanovic S, Vukovic D, Djukic S, Cirkovic I, Svabic-Vlahovic M. Long-term analysis of Listeria monocytogenes vaginal carriage frequency in Belgrade, Serbia (short communication). Acta Microbiol Immunol Hung. 2007;54(2):195-9.
Soni DK, Singh DV, Dubey SK. Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples. J Microbiol. 2015;53(9):653-60.
Egal ES, Ardeshir A, Mariano FV, Gondak RO, Montalli VA, dos Santos HT, et al. Contribution of Endemic Listeriosis to Spontaneous Abortion and Stillbirth in a Large Outdoor-housed Colony of Rhesus Macaques (Macaca mulatta). J Am Assoc Lab Anim Sci. 2015;54(4):399-404.
Shindang J, Shindang C, Ekwempu A. Incidence of Listeria monocytogenes and Other Bacteria in Spontaneous Abortion Cases in Jos. Nigerian Journal of Biotechnology. 2013;25.
Kaur S, Malik SV, Vaidya VM, Barbuddhe SB. Listeria monocytogenes in spontaneous abortions in humans and its detection by multiplex PCR. J Appl Microbiol. 2007;103(5):1889-96.
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| Issue | Vol 10 No 5 (2018) | |
| Section | Original Article(s) | |
| Keywords | ||
| Listeria monocytogenes Pregnant women Antimicrobial susceptibility Serotyping Virulence genes | ||
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