Diversity of lactic acid bacterial in inasua fermentation

  • Ferymon Mahulette Study Program of Microbiology, Graduate School of Bogor Agricultural University, Bogor, Indonesia
  • Nisa Rachmania Mubarik Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia
  • Antonius Suwanto Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia
  • Widanarni Widanarni Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, Indonesia
Keywords: Denaturing gradient gel electrophoresis, Dominance index, Fermented fish, Succession

Abstract

Background and Objectives: Inasua is one of the traditional fermented fish products in Maluku, Indonesia. There are twotypes of inasua, i.e. with and without sap. The research aimed to study the succession of lactic acid bacteria (LAB) duringfermentation and microbial composition in inasua.Materials and Methods: The sample of inasua was taken from two traditional producers in Layeni village, Ceram Island.The diversity of lactic acid bacteria was analyzed based on the 16S rRNA gene sequence.Results: The succession of lactic acid bacteria was strongly influenced by the physicochemical characteristics during fermentation.Lactobacillus plantarum was found dominant in both inasuas fermentation processes. At end of fermentation,L. plantarum was still found dominant in inasua with sap while inasua without sap was dominated by Leuconostoc mesenteroides.In addition, Lactobacillus paracasei (LAB) was found only in inasua with sap. The result of Denaturing Gradient Gel Electrophoresis (DGGE) revealed that Lactobacillus was the dominant bacteria in inasua with sap while Staphylococcuswas dominant in inasua without sap.Conclusion: Inasua with sap was found with higher bacterial diversity index and lower evenness and dominance indices, aswell as more complex LAB succession pattern during fermentation and bacterial composition, as opposed to inasua withoutsap.

References

Mahulette F, Mubarik NR, Suwanto A, Widanarni. Isolation and characterization of lactic acid bacteria from inasua. J Trop Biodi Biotechnol 2016; 1(2): 71-76.

Noonpakdee W, Jumriangrit P, Wittayakom K, Zendo J, Nakayama J, Sonomoto K, Panyim S.Two-peptide bacteriocin from Lactobacillus plantarum PMU 33 strain isolated from som-fak, a Thai low salt fermentation fish product. Asia Pac J Mol Biol Biotechnol 2009; 17(1): 19-25.

Pringsulaka O, Thongngam N, Suwannasai N, Atthakor W, Pothivejkul K, Rangsiruji A. Partial characterization of bacteriocins produced by lactic acid bacteria isolated from Thai fermented meat and fish product. Food Cont 2012; 23: 547-55

Saisithi P. (1994). Traditional fermented fish: Fish sauce production. In : Fisheries Processing: Biotechnological Application. Ed, AM Martin. Chapman and Hall, London. UK, pp. 111-131

Marui J, Boulom S, Panthavee W, Momma M, Kusumoto K, Nakahara K, Saito M. Culture independent analysis of the bacterial community during fermentation of pa-som, a traditional fermented fish product in Laos. Food Sci. Technol 2014; 80: 1109-1115

Fan L, Song J. (2013). Antimicrobial microbes-bacteriocin producing lactic acid bacteria.. In: Microbial pathogens and strategies for combating them: science, technology and education. Ed, A Mendez-Vilas. Formatex Research Center, Badajoz. Spain, pp. 899-909.

Marchesi JR, Sato T, Weigtman AJ, Martin TA, Fry JC, Hiom SJ, Wade WG. Design and evaluation of useful bacterium specific PCR primer that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 1998; 64: 795-799.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likehood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731-2739.

Overeas L, Fome L, Daae FL. Distribution of bacterioplankton in meromictic lake saelevannet as determined by denaturing gradient gel electrophoresis of PCR amplified gene fragment coding for 16S rRNA. Appl Environ Microbiol 1997; 63: 3367-3373.

Muyzer G, de Wall EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 1993; 59(3): 695-700.

Felsenstein J. Confidence limits on phylogenies: an approach using the bootsrap. Evol. 1985; 39: 783-791.

Hill TCJ, Kerry A, Harris JA, Moffett BF. Using ecological diversity measures with bacterial cummunities. FEMS Microbiol Ecol 2003; 43: 1-11.

Beckner M, Ivey ML, Phister TG. Microbial contamination of fuel ethanol fermentations. Letters Appl Microbiol 2011; 53: 387-394.

Reale A, Renzo TD, Rossi F, Zotta T, Iacumin L, Preziuso M, Parente E, Sorrentino E, Coppola F. Tolerance of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus strain to stress factor encountered in food processing and in the gastro-intestinal tract. Food Sci Technol. 2015; 60: 721-728.

Carr JF, Chill D, Maida N. The lactic acid bacteria : a literature survey. Crit Rev Microbiol 2002; 28(4): 281-370.

Gold RS, Meagher MM, Hutkins R, Conway T. Ethanol tolerance and carbohydrate metabolism in Lactobacilli. J Ind Microbiol 1992; 10: 45-54.

Nair PS, Surendran PK. Biochemical characterization of lactic acid bacteria isolated from fish and prawn. J. Cult Collect 2004; 4: 48-52.

Jonsson S, Clausen E, Raa J. Amino acid degradation by a Lactobacillus plantarum strain from fish. Syst Appl Microbiol 1983; 4: 148-154.

Todorov SD, Franco BDG. Lactobacillus plantarum: characterization of the species and application in food production. Food Rev Int 2010; 26: 205-229.

Verdenelli MC, Ghelfi F, Silvi S, Orpianesi C, Cecchini C, Cresci A. Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces. Eur J Nutr. 2009; 48(6): 355-363.

Dicks LMT, Endo A. Taxonomic status of lactic acid bacteria in wine and key characteristics to differentiate species. S Afr J Enol Vitic 2009; 30(1): 72-90.

Njoki WJ, Boga HI, Kutima PM, Maina MJ, Kadere TT. Probiotic potential of lactic acid bacteria from coconut (Cocos nucifera) wine (mnazi) in Kenya. Int J Life Sci Res 2015; 3(1): 113-120.

Urbina JAS, Teran FR. Microbiology and biochemistry of traditional palm wine produced around the world. Int Food Res J 2014; 21(4): 1261-1269.

Paludan-Muller CP. 2001. The microbiology of low salt fermented fish product. Danish Technical University. Lyngby. Denmark.

Panda SH, Ray RC, El Sheikha AF, Montet D, Worawattanamateekul W. Fermented fish and fish product. Aquacult Microbiol Biotechnol 2011; 2: 132-172.

Kung HF, Tsai YH, Chang SC, Hong TY. Biogenic amine content, histamine forming bacteria, and adulteration of pork in tuna sausage product. J Food Protec 2012; 75(10): 1814-1822.

Herrero MMH., Sagues AHR, Jerez JJL, Ventura MTM. Halotolerant and halophilic histamine-forming bacteria isolated during the ripening of salted anchovies (Engraulis encrasicholus). J Food Protec 1999; 62(5): 509-514.

Maeno S, Dicks L, Nakagawa J, Endo A. Lactobacillus apinorum belongs to the fructofilic lactic acid bacteria. Biosci Microbio Food Health 2017 (Advance Publication).

Atputharajah JD, Widanapathirana S, Samarajewa U. Microbiology and biochemistry of natural fermentation palm sap. Food Microbiol 1986; 3: 273-280.

Escalante A, Gomez MG, Hernandez G, Aguilar MSC, Munguia AL, Gosset G, Bolivar F. Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage using a polyphasic approach. Int. J. Food Microbiol 2008; 124: 126-134.

Irisawa T, Okada S. Lactobacillus sucicola sp. Nov., a motil lactic acid bacterium isolated from oak tree (Quercus sp) sap. Int J Syst Evol Microbiol 2009; 59: 2662-2665.

Hernandez RJA, Alvarez JAR, Encinas CV, Miceli FAG, Gonzalez HC, Marsch R, Talavera TA, Dendooven L. The bacterial community in ‘taberna’, a traditional beverage of Southern Mexico. Letters Appl Microbiol 2010; 51: 558-563.

Lyhs U, Bjorkroth J, Hyytia E, Korkeala H. The spoilage flora of vacuum-packaged, sodium nitrite or potassium nitrate, cold smoked raibouw trout stored at 40C or 80C. Int. J. Food Microbiol 1998; 45: 135-142.

Published
2018-11-23
How to Cite
1.
Mahulette F, Mubarik N, Suwanto A, Widanarni W. Diversity of lactic acid bacterial in inasua fermentation. IJM. 10(5):314-23.
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Original Article(s)