Consortium inoculum of five thermo-tolerant phosphate solubilizing Actinomycetes for multipurpose biofertilizer preparation
AbstractBackground and Objectives: Alkaline pH of the soil facilitates the conversion of phosphate present in phosphate fertilizer applied in the field to insoluble phosphate which is not available to plants. Problem of soluble phosphate deficiency arises, primarily due to needless use of phosphate fertilizer. We sought to biofertilizer with the thermo-tolerant phosphate solubilizing actinomycetes consortium that could convert insoluble phosphate to soluble phosphate at wider temperature range.Materials and Methods: In the present investigation consortium of five thermo-tolerant phosphate solubilizing actinomycetes was applied for preparation of inoculum to produce multipurpose bio-fertilizer. Phosphates solubilizing thermo-tolerant 32 actinomycetes strains were processed for identification with the use of PIBWIN software and were screened for phosphate solubilizing activity.Results: Amongst these five actinomycetes were selected on the basis of their ability to produce cellulase, chitinase, pectinase, protease, lipase, amylase and phosphate solubilizing enzymes. Ability to produce these enzymes at 28°C and 50°C were examined. Biofertilizer was prepared by using agricultural waste as a raw material. While preparation of bio-fertilizer the pH decreased from 7.5 to 4.3 and temperature increased up to 74°C maximum at the end of 4th week and in subsequent week it started to decline gradually till it reached around 50°C, which was found to be stable up to eighth week. This thermo-tolerant actinomycetes consortium released soluble phosphate of up to 46.7 µg ml-1.Conclusion: As the mesophilic organisms die out at high temperature of composting hence thormo-tolerant actinomycetes would be the better substitute for preparation of phosphate solubilizing bio-fertilizer with added potential to degrade complex macromolecules in composting.
Pikovskaya R. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiology 1948; 17: 362-370.
Richardson AE, Hadobas PA, Hayes JE. Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate. Plant J 2001; 25:641-649.
Rodriguez H, Fraga R. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 1999; 17:319-339.
Iwai T, Takahasi M, Oda K, Terada Y, Yoshida KT. Dynamic changes in the distribution of minerals in relation to phytic acid accumulation during rice seed development. Plant Physiol 2012; 160: 2007-2014.
Del Campillo MC, Van der Zee, Torrent J. Modeling long-term phosphorus leaching and changes in phosphorus fertility in excessively fertilized acid sandy soils. Eur J Soil Sci 1999; 50:391-399.
Delvasto P, Valverde A, Ballester A, Igual IM, Muñoz JA, González F, et. al. Characterization of brushite as a re-crystallization product formed during bacterial solubilization of hydroxyapatite in batch cultures. Soil Biol Biochem 2006; 38: 2645-2654.
Biswas DR, Narayanasamy G. Rock phosphate enriched compost: an approach to improve low-grade Indian rock phosphate. Bioresour Technol 2006; 97: 2243-2251.
Yang SS, Chen KS. Application of thermophilic microbes for preparing biofertilizers. Plant Prot Bull Spe Publ New 2003; 5: 267-291.
Zayed G, Abdel-Motaal H. Bio-active composts from rice straw enriched with rock phosphate and their effect on the phosphorous nutrition and microbial community in rhizosphere of cowpea. Bioresour Technol 2005; 96:929-935.
Yang SS. Application of microbial fertilizers on the three objectives agriculture. In: Chou, C.H., Yang, S.S. (Eds.), Challenge of three objectives in agriculture. council of agriculture, southern Taiwan joint services center of executive Yuan, institute of biotechnology of national pingtung university of science and technology, department of biochemical science and technology of national Taiwan university, Taiwan, 2003; pp. 265-292.
Ponmurugan P, Gopi C. In vitro production of growth regulators and phosphatase activity by phosphate solubilizing bacteria. Afr J Biotechnol 2006; 5:348-350.
Gupta R, Rekha S, Aparna S, Kuhad R. A modified plate assay for screening phosphate solubilizing microorganims. J Gen Appl Microbiol 1994; 40:255-260.
Williams ST, Goodfellow M, Wellington EMH, Vickers JC, Alderson G, Sneath PHA, et al. A probability matrix for identification of some Streptomycetes. J Gen Microbiol 1983; 129:1815-1830.
Williams ST, Sharpe ME, Holt JG. Bergey’s Manual of Systematic Bacteriology vol. 4 1989; Williams and Wilkins, London.
Bryant TN. PIBWIN - software for probabilistic identification. J Appl Microbiol 2004; 97:1326-1327.
Williams ST, Locci R, Vickers JC, Schofield GM, Sneath PHA, Mortimer AM. Probabilistic identification of Streptoverticillium species. Microbiology 1985; 131: 1681-1689.
Langham, C.D., S. T. Williams., P. H. A. Sneath, A. M. Mortimer. New probability matrices for identification of Streptomyces. J Gen Microbiol 1989; 135: 121-133.
Kämpfer, P and R. M. Kroppenstedt. Probabilistic identification of Streptomyces using miniaturized physiological tests. J Gen Microbiol 1991; 137: 1893-1902.
Tsai SH, Liu CP, Yang SS. Microbial conversion of food wastes for biofertilizer production with thermophilic lipolytic microbes. Renew Energy 2007; 32:904-915.
Lee YJ, Kim BK, Lee BH, Jo KI, Lee NK, Chung CH, et al. Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresour Technol 2008; 99:378-386.
Mandels M, Medeiros JE, Andreotti RE, Bissett FH. Enzymatic hydrolysis of cellulose: evaluation of cellulase culture filtrates under use conditions. Biotechnol Bioeng 1981; 23:2009-2026.
Kammoun R, Naili B, Bejar S. Application of a statistical design to the optimization of parameters and culture medium for α-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product). Bioresour Technol 2008; 99: 5602-5609.
Mahanta N, Gupta A, Khare SK. Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate. Bioresour Technol 2008; 99: 1729-1735.
Reddy MS, Kumar S, Babita K, Reddy MS. Biosolubilization of poorly soluble rock phosphates by Aspergillus tubingensis and Aspergillus niger. Bioresource Technology 2002; 84: 187-189.
Nautiyal CS. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 1999; 170:265-270.
Olsen SR, Sommers LE. Phosphorus. In: Page, A.L., Miller, R.H., Keeney, D.R. (Eds.), methods of soil analysis, part 2, chemical and microbial properties. 2nd ed. 1982; American Society of Agronomy, Madison, Wisconsin, pp. 403-430.
Jackson M (1973). Soil chemical analysis. Prentice Hall of India, New Delhi, India.
Cheng-Hsiung C, Shang-Shyng Y. Thermo-tolerant phosphate solubilizing microbes for multi-functional biofertilizer preparation. Bioresour Technol 2009; 100:1648-1658.
Hamdali H, Bouizgarne B, Hafidi M, Lebrihi A, Virolle MJ, Ouhdouch Y. Screening for rock phosphate solubilizing Actinomycetes from Moroccan phosphate mines. App Soil Ecology 2008; 38: 12-19.
Caroline CM. Rock phosphate solubilizing and cellulolytic actinomycete isolates of earthworm casts. Environ Manage 1994; 18:257-261.
Caroline CM. Rock phosphate solubilizing Streptosporangium isolates from casts of tropical earthworms. Soil Biol Bioche 1997; 29(3-4): 381-385.
Goodfellow M, Williams ST. Ecology of actinomycetes. Annu Rev Microbiol 1983; 37:189-216.
Chater KF. Genetics of differentiation in Streptomyces. Annu Rev Microbiol 1993;47:685-713.
Chunqiao X, Chi R, He H, Qiu G, Wang D, Zhang W. Isolation of phosphate-solubilizing fungi from phosphate mines and their effect on wheat seedling growth. App Biochem Biotechnol 2009; 159: 330-342.
Baas P, Bell C, Mancini LM, Lee MN, Conant RT, Wallenstein MD. Phosphorus mobilizing consortium Mammoth P enhances plant growth. Peer J 2016; 4:e2121.
Pandey A, Trivedi P, Kumar B, Palni LMS. Characterization of a phosphate-solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a sub-alpine location in the Indian central Himalaya. Curr Microbiol 2006; 53:102-107.
Indiragandhi P, Anandham R, Madhaiyan M, Sa TM. Characterization of plant growth promoting traits of bacteria isolated from larval guts of diamondback moth Plutella xylostella (Lepidoptera: Putellidae). Curr Microbiol 2008; 56(4):327-333.
Tahvonen RT, Avikainen H. The biological control of seed-borne Alternaria brassicicola of cruciferous plants with a powdery preparation of Streptomyces sp. J Agri Sci Finl 1987; 59:199-208.
Cross JV, Polonenko DR. An industry perspective on registration and commercialization of bio-control agents in Canada. Can J Plant Pathol 1996;18:446-454.
Tuomi T, Heino M, Rosenqvist H, Nordstro K, Laakso S. Fiber fractions from processing of barley in production and conservation of a biologic control agent. Appl Biochem Biotechnol 2001; 94:135-145.