Articles

Chemical composition and antimicrobial activity of Satureja hortensis and Trachyspermum copticum essential oil

Abstract

Background and Objectives: The aim of this study was to evaluate the chemical composition and antimicrobial activity of Satureja hortensis and Trachyspermum copticum essential oils against different kinds of microorganisms in vitro.
Material and Methods: The antimicrobial activity was evaluated by micro broth dilution assay and analysis of essential oils chemical composition by GC and GC/MS analysis.
Results: Thymol, p-cymene, γ-terpinene and carvacrol were the main components of S. hortensis oil while thymol, γ-terpinene, and o-cymene were the major components of T. copticum oil. Two essential oils exhibited strong antimicrobial activity but the antimicrobial activity of T. copticum oil was higher than that of S. hortensis oil.
Conclusion: Thymol as a main component of oils plays an important role in antimicrobial activity.

 

Zargari A (1990). Medicinal plants. Tehran: Tehran University Press.

Hussein GH, Miyashiro H, Nakamura N, Hattori M, Kakiuchi N, Shimotohno K. Inhibitory effects of Sudanese medicinal plant extracts on hepatitis C virus (HCV) protease. Phytother Res 2000; 14: 510-516.

Thangam C, Dhananjayan R. Antiinflammatory potential of the seeds of Carum copticum Linn. Ind J Pharmacol 2003; 34: 388-391.

Rasooli I, Fakoor MH, Yadegarinia D, Gachkar L, Allameh A, Rezaei MB. Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. Int J Food Microbiol 2008; 122: 135-139.

Anis M, Iqbal M. Antipyretic utility of some Indian plants in traditional medicine. Fitoterapia 1986; 57: 52-55.

Mathew N, Misra-Bhattacharya S, Perumal V, Muthuswamy K. Antifilarial Lead molecules isolated from Trachyspermum ammi. Molecules 2008; 13: 2156-2168.

Dashti-Rahmatabadi MH, Hejazian SH, Morshedi A, Rafati A. The analgesic effect of Carum copticum extract and morphine on phasic pain in mice. J Ethnopharmacol 2007; 109: 226-228.

Kaur T, Bijarnia RK, Singla SK, Tandon C.In vivo efficacy of Trachyspermum ammi anticalcifying protein in urolithiatic rat model. J Ethnopharmacol 2009; 126:459-462.

Hejazian SH, Mosaddegh MH, Dashti Rahmatabadi H. Antinociceptive effects of Carum copticum extracts in mice using formalin test. World Applied Sciences Journal 2008; 34: 388-391.

Bera D, Lahiri D, Nag A. Novel natural antioxidant for stabilization of edible oil: the ajowan (Carum copticum) extract case. Journal of the American Oil Chemists Society JAOCS 2004; 81: 169-172.

Khajeh M, Yamini Y, Sefidkon F, Bahramifar N.Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem 2004; 86: 587-591.

Sahaf BZ, Moharramipour S, Meshkatalsadat MH.Chemical constituents and fumigant toxicity of essential oil from Carum copticum against two stored product beetles. Insect Science 2007; 14: 213-218.

Srivastava M, Saxena A, Baby P. GC–MS investigation and antimicrobial activity of the essential oil of Carum copticum Benth & Hook. Acta Alimentaria 1999; 28:291-295.

Mohagheghzadeh A, Faridi P, Ghasemi Y.Carum copticum Benth & Hook essential oil chemotypes. Food Chem 2007; 100: 1217–1219.

Masada Y (1976). Analysis of Essential Oils. Halsted: New York, p.118.16. Shojaaddini M, Moharramipour S, Sahaf BZ. Fumigant toxicity of essential oil from Carum copticum against Indian meal moth, Plodia interpunctella. JPPR 2008;48: 411-418.

Minija J, Thoppil JE. Essential oil composition of Trachyspermum ammi (L) Sprague from South India. Indian J Pharmaceut Sci 2002; 64: 250-251.

Hajhashemi V, Sadraei H, Ghannadi AR, Mohseni M.Antispasmodic and anti-diarrhoeal effect of Satureja hortensis L. essential oil. J Ethnopharmacol 2002; 71:187-192.

Uslu C, Karasen RM, Sahin F, Taysi S, Akcay F.Effects of aqueous extracts of Satureja hortensis L. on rhinosinusitis treatment in rabbit. J Ethnopharmacol 2003; 88: 225-228.

Sahin F, Karaman I, Gulluce M, Ogutcu H, Sengul M, Adiguzel A, et al. Evaluation of antimicrobial activities of Satureja hortensis L. J Ethnopharmacol 2003; 87:61-65.

Adams RP (2001). Identification of essential oil by gas chromatography/quadrupole mass spectroscopy, Allured Publishing Corporation, Carol Stream, IL, USA.

Marchetti O, Moreillon P, Glauser M, Bille J, Sanglard D. Potent synergism of the combination of fluconazole and cyclosporine in Candida albicans. Antimicrob Agent Chemother 2000; 44: 2373-2381.

CLSI (2009). Methods for dilution Antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard M7-A8, Eighth Edition, Wayne, PA.

Carson CF, Mee BJ, Riley TV. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrob Agents Chemother 2002; 48: 1914-1920.

Mahboubi M, Ghazian Bidgoli F. Antistaphylococcal activity of Zataria multiflora essential oil and its synergy with vancomycin. Phytomedicine 2010; 17: 548-550.

Delgado B, Fernandez PS, Palop A, Periago PM. Effect of thymol and cymene on Bacillus cereus vegetative cells evaluated through the use of frequency distributions. Food Microbiology 2004; 21: 327-334.

Ultee A, Gorris LGM, Smid EJ. Bactericidal activity of carvacrol towards the food-borne pathogen Bacillus cereus. J Appl Microbiol 1998; 85: 211-218.

Ultee A, Bennik MHJ, Moezelar R. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol 2002; 68: 1561-1568.

Lambert RJW, Skandamis PN, Coote PJ, Nychas GJE.A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol 2001; 91: 453-462.

Ettayebi K, El-Yamani J, Rossi-Hassani BD. Synergistic effects of nisin and thymol on antimicrobial activitiesin Listeria monocytogenes and Bacillus subtilis. FEMS Microbiol Lett 2000; 183: 191-195.

Sivropoulou A, Papanikolaou E, Nikolaou C, Kokkini S, Lanaras T, Arsenakis M. Antimicrobial and cytotoxic activities of Origanum essential oils. J Agric Food Chem 1996; 44: 1202-1205.

Cristani M, Arrigo MD, Mandalari G, Castelli F, Sarpietro MG, Micieli D, et al. Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity. J Agric Food Chem 2007; 55: 6300-6308.33. Cox SD, Mann CM, Markham JL. Interactions between components of the essential oil of Melaleuca alternifolia. J Appl Microbiol 2001; 91, 492-497.

Juven BJ, Kanner J, Schved F, Weisslowicz H. Factors that interact with the antibacterial action of thyme essential oil and its active constituents. J Appl Bacteriol 1994; 76: 626- 631. 35. Carson CF, Riley TV. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J Appl Bacteriol 1995; 78: 264-269.

Griffin SG, Wyllie SG, Markham JL, Leach DN. The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flav Fragr J 1999; 14: 322-332.

Cosentino S, Tuberoso CI, Pisano B, Satta M, Mascia V, Arzedi E, et al. In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett Appl Microbiol 1999; 29: 130-135.

Magena T, Muyima NYO. Comparative evaluation of the antimicrobial activities of essential oils of Artemisia afra, Pteronia incana and Rosmarinus officinalis on selected bacteria and yeast strains. Lett Appl Microbiol 1992; 28: 291-296.

Mmbengwa V, Samie A, Gundidza M, Matikiti V, Ramalivhana NJ, Magwa ML. Biological activity and phytoconstituents of essential oil from fresh leaves of Eriosema englerianum. African J Biotech 2009; 8: 361-364.

Eftekhar F, Yousefzadi M, Azizian D, Sonboli A, Salehi P. Essential oil composition and antimicrobial activity of Diplotaenia damavandica. Z. Naturforsch C 2005; 60: 821-825.

Mourey A, Canillac N. Anti-Listeria monocytogenes activity of essential oils components of conifers. Food Control 2002; 13: 289-292.

Roman A, Weir U, Bloomfield SF.Antimicrobial effects of tea-tree oil and its major components on Staphylococcus aureus, Staph. epidermidis and Propionibacterium acnes. Lett Appl Microbiol 1995;

: 242-245.

Pasi S, Harvala E, Kretsi O, Chinou E, Chinou I. In vitro antimicrobial activity of natural products from Eucalyptus growing in Greece and mechanism of action. Clinic Microbial Infection 2001; 7: 1-394.

Lima IO, Oliveira RAG, Lima EO, Souza EL, Farias NP, Navarro DF. Inhibitory effect of some phytochemicals in the growth of yeasts potentially causing opportunistic infections. Brazilian Journal of Pharmaceutical Sciences 2005; 41: 199-203.

Andrews RE, Parks LW, Spence KD. Some effects of douglas fir terpenes on certain microorganisms. Appl Environ Microbiol. 1980; 40: 301-304.

Wright W 1991. Artemisia (Medicinal and Aromatic Plants - Industrial Profiles). Artemisia. Maffei M (Ed)2002. Vetiveria. The genus Vetiveria. Taylor and Francis NY.

Onawunmi GO, Yisak W, Ogunlana EO. Antibacterial constituents in the essential oil of Cymbopogan citrates (DC) Stapb. J Ethnopharmacol 1984; 12: 279-286.

Vaara M.Agents that increase the permeability of the outer membrane. Microbiol Mol Biol Rev 1992; 56: 395-411.

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Keywords
Antimicrobial activity Thymol Satureja hortensis Trachyspermum copticum

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How to Cite
1.
Mahboubi M, Kazempour N. Chemical composition and antimicrobial activity of Satureja hortensis and Trachyspermum copticum essential oil. Iran J Microbiol. 1;3(4):194-200.