Effectiveness of an inactivated paratuberculosis vaccine in Iranian sheep flocks using the Mycobacterium avium subsp paratuberculosis 316F strain
AbstractBackground and Objectives: Paratuberculosis (PTb) (John's disease) is an incurable chronic intestinal infection that mainly affects ruminants. PTb is caused by Mycobacterium avium subspecies paratuberculosis (MAP) with a global distribution. Despite evidences on MAP contribution in Crohn's disease its causal role is still a matter of controversy. In ruminant farming, vaccination is broadly accepted as an effective control measure of PTb. This article describes preparation and field trial of an inactivated PTb vaccine made from the MAP 316F strain.Materials and Methods: Formulation of the vaccine was conducted based on the method traditionally used in the UK. Identity of the MAP strain was authenticated by PCR-IS900 and PCR-F57 tests. In the field, a group of 100 lambs (3-8 weeks old) were subcutaneously inoculated with the vaccine preparation under study. These animals, pre-vaccination, were all PTb ELISA negative. Serum level of antibody was determined by ELISA on days 0, 30, 60, 120 and 240, post-vaccination.Results: In PCR-900 and PCR-F57, the MAP 316F strain produced two fragments of 560 and 704 bp length respectively, a confirmation of its identity as MAP bacterium. In the field trial and at the arranged time intervals, the achieved blood serum levels of antibody, attributable to the vaccine formulation, displayed considerably high values.Conclusion: Given that the PTb-caused economical losses in the Iranian environment are dramatically high and also the fact that future of state policy on control of PTb remains unknown, we belive vaccination of animals is the best recommendable practice.
Park HT, Yoo HS. Development of vaccines to Mycobacterium avium subsp. paratuberculosis infection.Clin Exp Vaccine Res 2016; 5: 108-116.
Groenendaal H, Zagmutt FJ, Patton EA, Wells SJ. Cost-benefit analysis of vaccination against Mycobacterium avium ssp. paratuberculosis in dairy cattle, given its cross-reactivity with tuberculosis tests. J Dairy Sci 2015; 98: 6070-6084.
Bastida F, Juste RA. Paratuberculosis control: A review with a focus on vaccination. J Immune Based Ther Vaccines 2011; 9: 8.
Sigurdsson B. A killed vaccine against paratuberculosis (johne's disease) in sheep. Am J Vet Res 1960; 21: 54-67.
Saxegaard, Fodstad F. Control of paratuberculosis (johne's disease) in goats by vaccination. Vet Rec 1985; 116: 439-441.
Windsor PA. Paratuberculosis in sheep and goats. Vet Microbiol 2015; 181(1-2): 161-169.
Lacasta D, Ferrer LM, Ramos JJ, Gonzalez JM, Ortin A, Fthenakis GC. Vaccination schedules in small ruminant farms. Vet Microbiol 2015; 181(1-2): 34-46.
Arrazuria R, Molina E, Garrido JM, Perez V, Juste RA, Elguezabal N. Vaccination sequence effects on immunological response and tissue bacterial burden in paratuberculosis infection in a rabbit model. Vet Res 2016; 47: 77.
Rathnaiah G, Zinniel DK, Bannantine JP, Stabel JR, Grohn YT, Collins MT, et al. Pathogenesis, molecular genetics, and genomics of Mycobacterium avium subsp. Paratuberculosis, the etiologic agent of johne's disease. Front Vet Sci 2017; 4: 187.
Baharsefat M, Amjadi A, Ahourai P, Yamini B, Entessar F, Hedayati H. paratuberculosis in goats and sheep in iran. Epidemiological, clinical, pathological features and laboratory diagnosis. Archive of Razi Institute 1972; 24: 49-61.
Talatchian M. First report of johne's disease in Iran. Bull Off Int Epizoot 1965; 64: 779-782.
Tell LA, Foley J, Needham ML, Walker RL. Comparison of four rapid DNA extraction techniques for conventional polymerase chain reaction testing of three mycobacterium spp. that affect birds. Avian Dis 2003; 47: 1486-1490.
Dohmann K, Strommenger B, Stevenson K, de Juan L, Stratmann J, Kapur V, et al. Characterization of genetic differences between Mycobacterium avium subsp. paratuberculosis type i and type ii isolates. J Clin Microbiol 2003; 41: 5215-5223.
Schonenbrucher H, Abdulmawjood A, Failing K, Bulte M. New triplex real-time pcr assay for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces. Appl Environ Microbiol 2008; 74: 2751-2758.
Bech-Nielsen S, Jorgensens J, Ahrens P, Feld N. Diagnostic accuracy of a Mycobacterium phlei-absorbed serum enzyme-linked immunosorbent assay for diagnosis of bovine paratuberculosis in dairy cows. J Clin Microbiol 1992; 30: 613-618.
Rosseels V, Huygen K. Vaccination against paratuberculosis. Expert Rev Vaccines 2008; 7: 817-832.
Singh SV, Singh PK, Singh AV, Sohal JS, Gupta VK, Vihan VS. Comparative efficacy of an indigenous 'inactivated vaccine' using highly pathogenic field strain of Mycobacterium avium subspecies paratuberculosis 'bison type' with a commercial vaccine for the control of capri-paratuberculosis in india. Vaccine 2007; 25: 7102-7110.
Eppleston J, Windsor PA. Lesions attributed to vaccination of sheep with gudair for the control of ovine paratuberculosis: Post farm economic impacts at slaughter. Aust Vet J 2007; 85: 129-133.