Iranian Journal of Microbiology 2017. 9(4):219-223.

Molecular detection of Bordetella holmesii in two infants with pertussis-like syndrome: the first report from Iran
Masoumeh Nakhost Lotfi, Vajiheh Sadat Nikbin, Omid Nasiri, Farzad Badmasti, Fereshteh Shahcheraghi


Background and Objectives: Bordetella holmesii is associated with a pertussis-like respiratory syndrome in healthy individuals and also a rare cause of septicaemia, endocarditis, pneumonia, and septic arthritis, mostly in immunocompromised patients. Culture technique and real-time PCR are 2 methods used to detect Bordetella spp.

Materials and Methods: In this study, 435 nasopharyngeal specimens of patients with suspected whooping cough were checked for the presence of B. holmesii using 2 methods of culture technique and real-time PCR.

Results: In this study, we detected hIS1001 and IS481 of B. holmesii in 2 infants suspected of having pertussis-like syndrome.

Conclusion: Our observations demonstrate that accurate diagnosis is needed to discriminate between B. holmesii and B. pertussis infections among pertussis cases; otherwise, it could lead to misestimating pertussis rate and vaccine efficacy.


Bordetella holmesii; Real-time PCR; HIS1001; Pertussis-like Infections

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