Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 99 101 EN Caterina Mammina Department of Sciences for Health Promotion and Mother-Child Care "G. D'Alessandro", University, Palermo, Italy. 2015 10 16 No Abstract https://ijm.tums.ac.ir/index.php/ijm/article/view/632 https://ijm.tums.ac.ir/index.php/ijm/article/download/632/405

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 102 107 EN Alireza Abdollahi Division of Pathology, Imam Khomeini Hospital, Tehran University of Medical Sciences Tehran, Iran. Saeed Shoar Department of Surgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. Nasrin Shoar School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. 2015 10 16 Background and Objectives: Recently, nosocomial infections have been discussed as a critical issue among intubated patients leading to significant morbidity and mortality. Hence, the pattern of microbiological colonization and antibiotic resistance are much valuable in this regard. We aimed to investigate the pattern of microorganism colonization and antibiotic resistance in patients with endotracheal tube or tracheostomy to propose a proper empirical antibiotic therapy in this setting. Materials and Methods: This cross sectional study was conducted among 880 patients admitted in Imam Khomeini hospital between 2008 and 2011 who were subsequently intubated or underwent tracheostomy due to insufficient self ventilation. Samples for microbiological cultures were obtained after extubation and then sent to the central laboratory for further assessment. Antibiograms and microbiological cultures were obtained for each sample. Results: Of 880 patients enrolled in this study, 531 (60.3%) were male and 349 (39.7%) were female. Nineteen different organisms were isolated including Acinetobacter (213, 24.2%), Pseudomonas aeruginosa (147, 16.7%), Staphylococcus aureus (106, 12%), Proteus mirabilis (90, 10.2%), and other organisms (324, 36.8%). Antibiotic resistance was mainly seen in Acinetobacter (ciprofloxacin, ceftazidim, cefepim, and penicillin), S. aureus (imipenem) and Klebsiella (pipracillin- tazobactam and ampicillin-sulbactam). Conclusion: This study represents the most common microorganisms colonizing tracheal tube of hospitalized patients and their pattern of antibiotic resistance. Acinetobacter was the most common microorganism isolated from endotracheal tube. Hence, it may be possible to initiate the empiric antibiotic treatment before the results of culture are become available. Ciprofloxacin was also the most prevalent antibiotic revealing resistant pattern. Moreover, most of the microorganisms were sensitive to imipenem and pipracillin-tazobactam. https://ijm.tums.ac.ir/index.php/ijm/article/view/630 https://ijm.tums.ac.ir/index.php/ijm/article/download/630/403

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 108 112 EN Reza Ranjbar Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Farzaneh-Mirsaeed Ghazi Department of Biochemistry, Payam Noor University, Tehran, Iran. Shohreh Farshad Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Giovanni-Maurizio Giammanco Department of Sciences for Health Promotion‘G. D’Alessandro’’, University, Palermo, Italy. Aurora Aleo Department of Sciences for Health Promotion‘G. D’Alessandro’’, University, Palermo, Italy. Parviz Owlia Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran. Nematollah Jonaidi Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Nourkhoda Sadeghifard Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran. Caterina Mammina Department of Sciences for Health Promotion‘G. D’Alessandro’’, University, Palermo, Italy. 2015 10 16 Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp.  is of increasing clinical concern specially in children worldwide. The aim of this study was to investigate the occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran. Materials and Methods: The study included all Shigella isolates recovered from pediatric patients aged less than 12 years admitted to a major pediatric hospital in Tehran, Iran, from 2008 to 2010. Bacterial identification, antimicrobial susceptibility testing, extended spectrum β-lactamases (ESBLs) screening and confirmatory tests were performed according to the standard guidelines. Conjugal transfer experiments and plasmid analysis were also carried out. Polymerase chain reaction and sequencing were used to identify the genetic determinants responsible for ESBL production. Results: Four out of 55 Shigella isolates, including three S. sonnei and one S. flexneri, showed an ESBL-positive phenotype. Plasmid transfer of the ESBL phenotype was successful for the S. flexneri isolate only. By PCR and sequencing, one S. sonnei isolate tested positive for the CMY-59 gene, while the other two S. sonnei and the S. flexneri isolates tested positive for the bla  CTX-M-15 genes. Conclusion: We found the prevalence of ESBL producing Shigella isolates was higher than detection rates observed in many other countries. Our finding raise concerns about the dissemination of ESBL among the strains of endemic S. sonnei throughout the country, because this species is now the most frequently isolated Shigella species in Iran and shigellosis by such strains in the community can pose a significant threat to patients and presents a challenge for disease management. https://ijm.tums.ac.ir/index.php/ijm/article/view/628 https://ijm.tums.ac.ir/index.php/ijm/article/download/628/401

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 113 119 EN Seyed-Mehdi Larrie-Bagha Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. Iraj Rasooli Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. Seyed-Latif Mousavi-Gargari Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. Zohreh Rasooli School of Medicine, Shahed University, Tehran-Iran. Shahram Nazarian Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. 2015 10 16 Background and Objectives: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 has been recognized as a major food borne pathogen responsible for frequent hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are important reservoirs of E. coli O157:H7, in which the organism colonizes the intestinal tract and is shed in the feces. Objective: Vaccination of cattle has significant potential as a pre-harvest intervention strategy for E. coli O157:H7. The aim of this study was to evaluate active and passive immunization against E. coli O157:H7 using a recombinant protein. Materials and Methods: The recombinant FepA protein induced by IPTG was purified by nickel affinity chromatography. Antibody titre was determined by ELISA in FepA immunized rabbits sera. Sera collected from vaccinated animals were used for bacterial challenge in passive immunization studies. Results: The results demonstrate that passive immunization with serum raised against FepA protects rabbits from subsequent infection. Conclusion: Significant recognition by the antibody of ferric enterobactin binding protein may lead to its application in the restriction of Enterobacteriaceae propagation. https://ijm.tums.ac.ir/index.php/ijm/article/view/626 https://ijm.tums.ac.ir/index.php/ijm/article/download/626/399

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 120 125 EN Ramachandranpillai Rajagopal Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India, 680 651. Govindapillai-Krishnan Nair Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India, 680 651. Mangattumuruppel Mini Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India, 680 651. Leo Joseph Kerala Agricultural University Poultry Farm, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India, 680 651. Mapranath-Raghavan Saseendranath Department of Preventive Medicine and Veterinary Epidemiology, College of Veterinary & Animal Sciences, Mannuthy, Thrissur, Kerala, India. 680 651. Koshy John Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookot, Lakkidi P.O., Wayanad, Kerala, India - 673 576. 2015 10 16 Background and Objectives: Biofilms are structural communities of bacterial cells enshrined in a self produced polymeric matrix. The studies on biofilm formation of Pasteurella multocida have become imperative since it is a respiratory pathogen and its biofilm mode could possibly be one of its virulence factors for survival inside a host. The present study describes a biofilm assay for P. multocida on inert hydrophilic material called bentonite clay. Materials and Methods: The potential of the organism to form in vitro biofilm was assessed by growing the organism under nutrient restriction along with the inert substrate bentonite clay, which will provide a surface for attachment. For quantification of biofilm, plate count by the spread plate method was employed. Capsule production of the attached bacteria was demonstrated by light microscopic examination following Maneval staining and capsular polysaccharide estimation was done using standard procedures. Results and Conclusion: The biofilm formation peaked on the third day of incubation (1.54 ×106  cfu/g of bentonite clay) while the planktonic cells were found to be at a maximum on day one post inoculation (8.10 ×108  cfu/ml of the broth). Maneval staining of late logarithmic phase biofilm cultures revealed large aggregates of bacterial cells, bacteria appearing as chains or as a meshwork. The capsular polysaccharide estimation of biofilm cells revealed a 3.25 times increase over the planktonic bacteria. The biofilm cells cultured on solid media also produced some exclusive colony morphotypes. https://ijm.tums.ac.ir/index.php/ijm/article/view/624 https://ijm.tums.ac.ir/index.php/ijm/article/download/624/397

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 126 131 EN Rashid Ramazanzadeh Cellular & Molecular Research Center and Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. Serveh Zamani Student Research Committee of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. Saman Zamani Student Research Committee of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. 2015 10 16 Background and Objectives: The Escherichia coli strains are greatly important in nosocomial and community acquired infections. The aim of this study was to determine the transmission of bacterial infections using genetic analysis. Materials and Methods: Two hundred and thirty Escherichia coli strains, isolated from different clinical samples, were characterized by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique. The results and the similarity between the strains were determined on the basis of Jaccard similarity coefficient in the SAHN program of the NTSYS-pc software. Results: The ERIC–PCR profiles allowed typing of the 230 isolates into 205 ERIC-types which were grouped into twenty main clusters (C01–C20).The first group makes up 4.34% of the total isolates. Out of the 230 isolates, 34.2% belonged to D phylogenic group which were associated with extra-intestinal samples. Conclusion: Our results showed high diversity in E. coli isolates indicating low rate of hospital infection in our university hospitals. The majority of the isolates belonged to the D phylogenic group. https://ijm.tums.ac.ir/index.php/ijm/article/view/622 https://ijm.tums.ac.ir/index.php/ijm/article/download/622/395

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 132 135 EN Abdullah Karimi Pediatric Infections Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Koroush Fakhimi-Derakhshan School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Farid Imanzadeh Departments of Pediatric Gastroenterology, Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Iran. Mohamad Rezaei School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Zahra Cavoshzadeh Pediatric Infections Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Saeid Maham Pediatric Infections Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2015 10 16 Objective: Childhood infectious diseases are one of the most known environmental pathogenic causes of childhood asthma. The high prevalence of both Helicobacter pylori infection and asthma in our country prompted us to assess anyprobable as- sociation between them in childhood. Methods: This cross-sectional study recruited 196 children aged 6 to 12 years old comprising 98 asthmatic (case group) and 98 healthy (control group) individuals. Urea breath test was performed for all of the children and H. pylori infection was compared between the two groups according to the urea breath test results. Results: Urea breath test was positive in 18 asthmatic (18.36) and 23 (23.36) healthy subjects but was not significantly dif- ferent between the case and controls (p = 0.380). Further analysis in the asthmatic group revealed association of H. pylori- infection with age (p < 0.001) and duration of asthma (p = 0.010). However, no significant correlation was found between sex, severity of asthma, controled asthma  or abnormal pulmonary function tests with H. pyloriinfection (p = 0.804, 0.512,0.854 and 0.292, respectively). Conclusion: Given the results of the study, H. pylori infection was not significantly differentbetween asthmatic and healthy children. In asthmatic patients, there was no significant association between H. pylori infection and sex, severity of disease, control status of disease andnormal or abnormal pulmonary function tests. H. Pylori infection had a significant association with increasing age and duration of asthma. https://ijm.tums.ac.ir/index.php/ijm/article/view/620 https://ijm.tums.ac.ir/index.php/ijm/article/download/620/393

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 136 141 EN Siji-Jinachandrannair Vijayakumari Central Tuber Crops Research Institute, Thiruvananthapuram - 695 017, Kerala, India. Nishanth-Kumar Sasidharannair Central Tuber Crops Research Institute, Thiruvananthapuram - 695 017, Kerala, India. Bala Nambisan Central Tuber Crops Research Institute, Thiruvananthapuram - 695 017, Kerala, India. Chellappan Mohandas Central Tuber Crops Research Institute, Thiruvananthapuram - 695 017, Kerala, India. 2015 10 16 Background and Objectives: Entomopathogenic nematodes, belonging to the family heterorhabditis and steinernematidae, are reported to be symbiotically associated with specific bacteria and the secondary metabolites produced by these bacteria possess antimicrobial activity. In this study, bacteria were isolated from nematodes belonging to the family rhabditidae, and the antimicrobial activity was tested against four bacteria viz. Bacillus subtilis MTCC 2756, Staphylococcus aureus MTCC 902, Escherichia coli MTCC 2622, and Pseudomonas aeruginosa MTCC 2642 and five fungi viz. Aspergillus flavus MTCC 183, Candida albicans MTCC 277, Fusarium oxysporum MTCC 284, Rhizoctonia solani MTCC 4634 and Penicillium expansum MTCC 2006. Materials and Methods: The isolated bacteria were cultured in nutrient broth (NB), Luria broth (LB) and Tryptic soya broth (TSB) at 25, 30 and 35ºC. Cell free culture filtrate was prepared by centrifugation and was separated into organic and aqueous fractions. Organic fraction was concentrated and tested for antimicrobial activity. Results: The culture filtrate of the bacteria isolated from the entomopathogenic Rhabditis sp. was found to possess antimicrobial activity against the four bacteria and five fungi tested. The bacterium grew well in TSB, LB and NB media though in TSB yield and activity were higher. Antimicrobial activity was higher at 30ºC as compared with 25 or 35ºC. HPLC analysis indicated major differences in peak areas and retention times at different temperatures. Increased number of peaks with higher peak areas was obtained at 30ºC. Conclusion: The study suggests that the bacteria could produce more bioactive molecules effective against medically and agriculturally important bacteria and fungi depending on culture media and temperature. Modified media could yield different types of molecules effective against diseases/disorders of plant, animals and humans. https://ijm.tums.ac.ir/index.php/ijm/article/view/618 https://ijm.tums.ac.ir/index.php/ijm/article/download/618/391

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 142 146 EN Davoud Esmaeilli Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AND Department of Bacteriology, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran. Ashraf Mohabati-Mobarez Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Ali Hatef-Salmanian National Institutes for Genetic Engineering and Biotechnology, Tehran, Iran. Ahmad Zavaran-Hosseini Dept. of Immunology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran. 2015 10 16 Background and Objectives: Helicobacter pylori is the causative agent of peptic ulcer disease and a co-factor in development of gastric malignancies. LPS are among toxic substances produced by H. pylori exhibiting low endotoxic activity compared to typical bacterial LPS. The aim of this study was to investigate bioactivity of LPS produced by different serotypes of Helicobacter pylori compared to Escherichia coli and Brucella abortus LPS. Materials and Methods: Bacterial LPS was extracted by the hot phenol-water method. Biological activities of LPS were determined via the limulus lysate assay, pyrogenic assay, and blood pressure and PBMC induction test in rabbits. Results: Biological activity of O serotype LPS of H. pylori was less than the biological activity of other H. pylori serotypes. Conclusion: Our data supported the hypothesis that the unique bacterial LPS of the O formulation of a multivalent H. pylori vaccine. https://ijm.tums.ac.ir/index.php/ijm/article/view/616 https://ijm.tums.ac.ir/index.php/ijm/article/download/616/389

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 147 152 EN Lee Seong Wei Faculty of Agro Based Industry, University of Malaysia, Kelantan Jeli Campus, 17600 Jeli, Kelantan, Malaysia. Wendy Wee Department of Fisheries Science, Faculty of Fisheries and Aqua-Industry, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia. 2015 10 16 Background and Objectives: This paper describes chemical composition and antimicrobial activity of Cymbopogon nardus citronella essential oil against Edwardsiella spp. (n = 21), Vibrio spp. (n = 6), Aeromonas spp. (n = 2), Escherichia coli (n = 2), Salmonella spp. (n = 2), Flavobacterium spp. (n = 1), Pseudomonas spp. (n = 1) and Streptococcus spp. (n = 1) isolated from internal organs of aquatic animals. Due to the ban of antibiotics for aquaculture use, this study was carried out to evaluate the potential of citronella essential oil as alternative to commercial antibiotic use against systemic bacteria in cultured aquatic animals. Materials and Methods: The essential oil of C. nardus was prepared by using the steam distillation method and the chemical composition of the essential oil was analyzed by gas chromatography–mass spectroscopy (GC–MS). Minimum inhibitory concentration (MIC) of the essential oil tested against bacterial isolates from various aquatic animals and ATCC type strains were determined using two-fold broth micro dilution method with kanamycin and eugenol as positive controls. Results: A total of 22 chemical compounds were detected in C. nardus essential oil with 6-octenal, 3, 7-dimethyl- or citronellal representing the major compounds (29.6%). The MIC values of the citronella oil ranged from 0.244 µg/ml to 0.977 µg/ml when tested against the bacterial isolates. Conclusion: The results of the present study revealed the potential of C. nardus essential oil as alternative to commercial antibiotics for aquaculture use. https://ijm.tums.ac.ir/index.php/ijm/article/view/614 https://ijm.tums.ac.ir/index.php/ijm/article/download/614/387

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 153 158 EN Razieh Shahbazi Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran. Roha Kasra-Kermanshahi Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran. Sara Gharavi Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran. Zahra Moosavi-Nejad Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran. Faezeh Borzooee Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran. 2015 10 16 Background and Objectives: Sodium dodecyl sulfate (SDS) is one of the main surfactant components in detergents and cosmetics, used in high amounts as a detergent in products such as shampoos, car wash soap and toothpaste. Therefore, its bioremediation by suitable microorganisms is important. Alkylsulfatase is an enzyme that hydrolyses sulfate -ester bonds to give inorganic sulfate and alcohol. The purpose of this study was to isolate SDS–degrading bacteria from Tehran city car wash wastewater, study bacterial alkylsulfatase enzyme activity and identify the alkylsulfatase enzyme coding gene. Materials and Methods: Screening of SDS-degrading bacteria was carried out on basal salt medium containing SDS as the sole source of carbon. Amount of SDS degraded was assayed by methylene blue active substance (MBAS). Results and Conclusion: Identification of the sdsA gene was carried by PCR and subsequent sequencing of the 16S rDNA gene and biochemical tests identified Pseudomonas aeruginosa. This bacterium is able to degrade 84% of SDS after four days incubation. Bacteria isolated from car wash wastewater were shown to carry the sdsA gene (670bp) and the alkylsulfatase enzyme specific activity expressed from this gene was determined to be 24.3 unit/mg . The results presented in this research indicate that Pseudomonas aeruginosa is a suitable candidate for SDS biodegradation. https://ijm.tums.ac.ir/index.php/ijm/article/view/612 https://ijm.tums.ac.ir/index.php/ijm/article/download/612/385

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 159 165 EN Mrunmaya Kumar Panda Department of Bioinformatics, North Orissa University, Takatpur, Baripada-757003, Odisha, India. Mahesh Kumar Sahu Department of Bioinformatics, North Orissa University, Takatpur, Baripada-757003, Odisha, India. Kumananda Tayung Department of Botany, North Orissa University, Takatpur, Baripada-757003, Odisha, India. 2015 10 16 Background and Objectives: Thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. Materials and Methods: In this study, thermophilic bacteria were isolated from hot spring of Tarabalo, India. A bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16S rRNA gene. The isolate was screened for protease and amylase activity. Phylogenetic affiliations and G+C content of the isolate was studied. Results: The bacterium with the ability to tolerate high temperatures was identified as Bacillus sp. both by morphology, biochemistry and sequencing of its 16S rRNA gene. BLAST search analysis of the sequence showed maximum identity with Bacillus amyloliquefaciens (99% similarity). Strain exhibited considerable protease activity. Phylogenetic analysis of the isolate revealed close affiliation with thermophilic Bacillus species. The G+C content was found to be 54.7%. Conclusion: The study confirmed that the isolated Bacillus sp. to be a true thermophile and could be a source of thermostable protease which can be exploited for pharmaceutical and industrials applications. https://ijm.tums.ac.ir/index.php/ijm/article/view/610 https://ijm.tums.ac.ir/index.php/ijm/article/download/610/383

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 166 171 EN Asghar Abdoli Department of Virology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran. Hoorieh Soleimanjahi Department of Virology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran. Masoumeh-Tavassoti Kheiri Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran. Abbas Jamali Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran. Hesam Sohani Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran. Mohsen Abdoli Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran. Hamid Reza Rahmatollahi Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran. 2015 10 16 Background and Objectives: Virosomes are Virus Like Particles (VLP) assembled in-vitro. Influenza virosomes maintain the cell binding and membrane fusion activity of the wild type virus but are devoid of viral genetic material or internal proteins. Influenza virosomes mimic the natural antigen presentation route of the influenza virus. Materials and Methods: Virosomes were prepared by membrane solubilization and reconstitution. Briefly, the Madine- Darby Canine kidney (MDCK) cell line was cultivated on microcarrier beads inoculated with influenza virus strain A/X-47 (H3N2). The culture medium was harvested and clarified. Subsequently, virus was concentrated and purified by ultrafiltration and ultracentrifugation. The purified viral membrane was dissolved by adding 375 μl of 200 mM 1, 2-dicaproyl-sn-glycero-3-phosphocholine  (DCPC)  in  HEPES-buffered  saline  (HBS).  Nucleocapsid  was  removed  by  ultracentrifugation. The supernatant consisting of phospholipids and glycoproteins of the influenza virus was reconstituted by removal of DCPC using overnight dialysis against Hank’s Buffered Saline (HBS) solution at 4°C. After dialysis, crude virosome preparation was layered over a discontinuous sucrose gradient in order to separate non-incorporated material from the reconstituted virus membranes. Results:  The  virosome  harvested  from  the  boundary  of  the  two  sucrose  layers  successfully  was  identified by  the Hemagglutination assay and western blotting. Conclusion: Use of a dialyzable short-chain phospholipid (DCPC) is an efficient procedure for solubilization and reconstitu- tion of influenza virus virosomes and has not caused structural changes in a major envelope glycoprotein (hemagglutinin protein) on the surface of virosome. https://ijm.tums.ac.ir/index.php/ijm/article/view/608 https://ijm.tums.ac.ir/index.php/ijm/article/download/608/381

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 172 176 EN Hassan Ehteram Department of Pathology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran. Amitis Ramezani Clinical Research and 6Vacination, Pasteur Institute of Iran, Tehran, Iran. Ali Eslamifar Clinical Research and 6Vacination, Pasteur Institute of Iran, Tehran, Iran. Masoomeh Sofian Tuberculosis and Pediatric Infectious Research Center, Arak University of Medical Sciences, Arak, Iran. Mohammad Banifazl Iranian Society for Supporting Patients with Infectious Diseases, Tehran, Iran. Shahin Ghassemi Firoozgar Hospital, Tehran University of Medical Sciences, Tehran, Iran. Arezoo Aghakhani Clinical Research and 6Vacination, Pasteur Institute of Iran, Tehran, Iran. Parisa Mashayekhi Department of Vaccination, Pasteur Institute of Iran, No 69, Pasture Ave., Tehran 13164, Iran. 2015 10 16 Background and Objectives: Hepatitis E virus (HEV) is a major public health concern in developing countries. HEV transmission occurs primarily by the fecal-oral route. It has also been reported that blood donors are potentially able to cause transfusion-associated hepatitis E in endemic areas. This study aimed to determine the seroprevalence of HEV infection among volunteer blood donors in Central province of Iran in 2012. Material and Methods: A total of 530 consecutive blood donor samples collected from Blood Transfusion Organization, Central Province of Iran. All samples were tested for the presence of IgG Hepatitis E antibody (anti-HEV) using enzyme- linked immunosorbent assay (ELISA). Results: From 530 blood donors, 91.9% were male and 8.1% were female. Overall, anti-HEV was found in 76 of 530 samples (14.3%). There was no significant difference in HEV seropositivity between the subjects regarding gender and area of residence (urban vs. rural). Anti-HEV was distributed among all age groups. Although people aged 31-50 years had the highest prevalence, but there was no statistical difference between the age groups. Conclusion: This study shows a relatively high prevalence of anti-HEV in the blood donors of Central province of Iran. More investigations are needed to assess the potential benefit of adding HEV screening of blood products to the current blood donor selection criteria. https://ijm.tums.ac.ir/index.php/ijm/article/view/606 https://ijm.tums.ac.ir/index.php/ijm/article/download/606/379

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 177 182 EN Fariba Jafari Department of Medical Parasitoloy and Mycology, Medical School, Zanjan University of Medical Sciences and Health Services. Abbasali Nourian Department of Medical Parasitoloy and Mycology, Medical School, Zanjan University of Medical Sciences and Health Services. Asghar Fazaeli Department of Medical Parasitoloy and Mycology, Medical School, Zanjan University of Medical Sciences and Health Services. Alireza Yazdinezhad Department of Pharmacognozy, Faculty of Pharmacy. Zanjan University of Medical Sciences and Health Services, Zanjan, Iran. Ali Haniloo Department of Medical Parasitoloy and Mycology, Medical School, Zanjan University of Medical Sciences and Health Services. 2015 10 16 Background and Objectives: Current chemotherapies of cutaneous leihmaniasis have faced to some problems and limitations; Development of new leishmanicidal drugs from different sources like herbal plants, are crucially important. The objective of the present study was evaluation of in vitro activity of Alkanna frigida extracts in comparison with glucantime against Leishmania major. Materials and Methods: L. major promastigotes were exposed to different concentrations of the A. frigida extracts, processed by ethyl acetate, ethanol, hexane and chloroform. The inhibitory effect, as the IC 50 , were calculated after 24, 48 and 72 hours  by linear regression analysis values of the concentrations employed. Results: The significant inhibition was observed after 24 and 48 hours with different concentrations of compounds (p < 0.05 in all tests). All extracts had potent activity against proliferation of the promastigotes, comparing to the untreated negative control. It could compete with the glucantime efficacy in some concentrations. Ethyl acetate and ethanol extractions showed potent IC   value, 106 µg/ml and 86 µg/ml, respectively. Hexane and chloroform extractions had poor efficacy after 24 hours;however, the efficacy increased after 48 and 72 hours. Conclusion: The results indicated that the A. frigida has appropriate inhibitory effects on the growth of L. major promastigotes in vitro and can be of herbal targets for further investigation in vivo. https://ijm.tums.ac.ir/index.php/ijm/article/view/604 https://ijm.tums.ac.ir/index.php/ijm/article/download/604/377

Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 603 603 2015 10 16 https://ijm.tums.ac.ir/index.php/ijm/article/view/603