Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 8 3 2016 10 04 193 202 EN Abbas Abdollahi Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran Shahla Mansouri Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran Jafar Amani Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mahdi Fasihi 3Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mohammad Moradi Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran 2016 03 12 2016 04 29 Background and Objectives: Brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. Because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against Brucella is desirable. In this study, we evaluated the immune responses induced by a designed recombinant chimera protein in murine model. Materials and Methods: Three immunodominant antigens of Brucella have been characterized as potential immunogenic and protective antigens including: trigger factor (TF), Omp31 and Bp26 were fused together by EAAAK linkers to produce a chimera (structure were designed in silico), which was synthesized, cloned, and expressed in E. coli BL21 (DE3). The purification of recombinant protein was performed using Ni-NTA agarose. SDS-PAGE and anti-His antibody was used for confirmation purified protein (Western blot). BALB/c immunization was performed by purified protein and adjuvant, and sera antibody levels were measured by ELISA. otted. Results: SDS-PAGE and Western blotting results indicated the similarity of in silico designing and in vitro experiments. ELISA result proved that the immunized sera of mice contain high levels of antibodies (IgG) against recombinant chimeric protein. Conclusion: The recombinant chimeric protein could be a potential antigen candidate for the development of a subunit vaccine against Brucella.   https://ijm.tums.ac.ir/index.php/ijm/article/view/948 https://ijm.tums.ac.ir/index.php/ijm/article/download/948/609