Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 8 5 2016 12 19 312 315 EN Nadi Maazi Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Shahram Jamshidi Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Payman Kayhani Department of Clinical Sciences, Faculty of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran Hassan Momtaz Department of Microbiology, Faculty of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran 2016 03 07 2016 10 21 Background and Objectives: Feline herpesvirus-1, feline calicivirus and Chlamydophila felis are the main causes of feline upper respiratory tract disease. This study was conducted to identify of FeHV-1, FCV and C. felis infections in domestic cat population and also to estimate the prevalence of each specific infection in Iran. Materials and Methods: The ocular conjunctiva and oropharyngeal specimens obtained from 80 cats were examined using PCR and reverse transcription PCR. Results: FeHV-1 was detected in 23 (28.8%), FCV in 2 (2.5%) and C. felis in 16 (20%) cats. Twelve cats(15%) had co-infection with 2 or 3 of the mentioned pathogens. Ocular lesions were the most common clinical signs in the FeHV-1 and C. felis infections whereas respiratory lesions were more observed with the FCV infections. It seems that there is an age-related tendency in the infected cats, meaning that the age of the C. felis positive cats was less than those with FeHV-1 and FCV infections. Conclusion: These results confirm the presence and show the prevalence of three major pathogens associated with upper respiratory tract disease for the first time in Iran.   https://ijm.tums.ac.ir/index.php/ijm/article/view/945 https://ijm.tums.ac.ir/index.php/ijm/article/download/945/667