Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 7 3 2015 11 25 168 172 EN Sakineh Seyed-Mohamadi Department of Aerobic Veterinary Bacteria, Razi Institute, Karaj, Iran. Soheila Moradi-Bidhendi Department of Microbiology, Razi Institute, Karaj, Iran. Keyvan Tadayon Department of Aerobic Veterinary Bacteria, Razi Institute, Karaj, Iran. Rainak Ghaderi Department of Aerobic Veterinary Bacteria, Razi Institute, Karaj, Iran 2015 11 24 Background and Objectives: Bacillus anthracis is one of the most homogenous bacteria ever described. Bacillus anthracis 17JB is a laboratory strain. It is broadly used as a challenge strain in guinea pigs for potency test of anthrax vaccine. Material and Methods: This work describes genetic characterization of B. anthracis 17 JB strain using the SNPs and MLVA genotyping. Results and Conclusion: In SNPs typing, the originally French 17JB strain represented the A. Br. 008/009 subgroup. In Levy's genotyping method, 843, 451 and 864 bp long fragments were identified at AA03, AJ03 and AA07 loci, respectively. In the vaccine manufacturer perspective these findings are much valuable on their own account, but similar research is required to extend molecular knowledge of B. anthracis epidemiology in Persia. https://ijm.tums.ac.ir/index.php/ijm/article/view/819 https://ijm.tums.ac.ir/index.php/ijm/article/download/819/503