Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 3 1 2011 03 15 21 25 EN H Jamalifar Departments of Drug and Food Control Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. HR Rahimi Toxicology Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. N Samadi Departments of Drug and Food Control Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. AR Shaverdi Biotechnology,Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Z Sharifian Department of Drug Regulatory Affairs, Iranian Food and Drug Administration, Tehran, Iran. F Hosseini Departments of Drug and Food Control Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. H Eslahi Departments of Drug and Food Control Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. MR Fazeli Departments of Drug and Food Control Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran AND Pharmaceutical Quality Assurance Research Center, Tehran, Iran. 2015 10 01 Background: Lactobacilli are the well known friendly bacteria for their probiotic activities against pathogens. The inhibitory activity of different strains of lactobacilli either obtained as commercial products or isolated from human feces was investigated against the clinical isolates of Pseudomonas aeruginosa. The isolates were selected as the most resistant strains when challenged with anti-pseudomonal antibiotics already in clinical practice. Materials and Methods: Both the plate spot test as well as the agar cup method were used for screening of Lactobacillus strains against Pseudomonas aeruginosa. Results: A Lactobacillus acidophilus strain isolated from feces of an Iranian child showed a strong anti-pseudomonal activity (90 percent after 72h incubation) against the multi-drug resistant clinical isolates while a Lactobacillus reuteri strain isolated from a commercial oral product resulted in relatively weak response and a Lactobacillus acidophilus strain isolated from a commercial vaginal product did not show any inhibitory activity. In a kinetic study the lactobacillus sensitive Pseudomonas aeruginosa showed a significant bacteriostatic activity in vitro in the presence of lactobacillus supernatants. Conclusion: Some lactobacilli exhibit significant inhibitory activity against the multidrug resistant clinical isolates of Pseudomonas aeruginosa. https://ijm.tums.ac.ir/index.php/ijm/article/view/80 https://ijm.tums.ac.ir/index.php/ijm/article/download/80/80