Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 4 1 2012 03 15 15 24 EN A Koluman National Food Reference Laboratory, Ankara, Republic of Turkiye AND Turkish Atomic Energy Authority, Saraykoy Nuclear Research and Training Center Ankara, Republic of Turkiye. G Celik Turkish Atomic Energy Authority, Saraykoy Nuclear Research and Training Center Ankara, Republic of Turkiye. T Unlu Ankara University, Veterinary Faculty, Dept. Microbiology, Ankara, Republic of Turkiye. 2015 10 16 Background and Objectives: The significant rise in food borne infections is mainly caused by Campylobacter spp., Salmonella serovars and Verotoxigenic Escherichia coli. As the emerging food borne pathogens cause disease, more studies have been conducted for rapid detection of these pathogens. The combination of immunomagnetic separation and polymerase chain reaction (IMS-PCR) is the most accurate and rapid test preferred by almost every researcher. Fourier Transform Infrared Spectroscopy (FTIR) is preferred for being a new, user friendly and rapid technique in microbiological analyses. The main aim of this study is to detect application of IMS-FTIR for Salmonella identification from foods in a short time with a higher sensitivity. Materials and Methods: Conventional Culture Technique (CC), IMS-CC, IMS-PCR and IMS-FTIR techniques were compared with each other for rapid detection in artificially contaminated minced beef with Salmonella Typhimurium, as of the 2nd, 4th and 8th hours of contamination. The method was evaluated in different food matrices and sensitivity, specifity and overall recovery was calculated. Results: The results indicate that IMS-FTIR can detect S. Typhimurium as of the 8th     hour with sensitivity of 95.6667, accuracy of 91.69329, false positive ratio of 0.04333 and overall recovery of  95.66%. Conclusion: It can be suggested that the IMS-FTIR method is capable of detecting S.Typhimurium in a short time with lower cost. https://ijm.tums.ac.ir/index.php/ijm/article/view/718 https://ijm.tums.ac.ir/index.php/ijm/article/download/718/485