Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 2 3 2010 09 15 152 156 EN S Ghodsi Biology Department, Faculty of Sciences, Alzahra University, Vanak, Tehran, IR Iran. S Gharavi Biology Department, Faculty of Sciences, Alzahra University, Vanak, Tehran, IR Iran. P Ghadam Biology Department, Faculty of Sciences, Alzahra University, Vanak, Tehran, IR Iran. 2015 10 01 Background and Objectives: Bacillus subtilis HBsu is a 10 kD heat-stable protein shown to be involved in binding to DNA and is encoded by the hbs gene. Large-scale production for biochemical analysis is achieved through cloning and expression of the recombinant protein. Materials and Methods: This gene was amplified from B. subtilis ATCC 6633 using PCR and cloned into pET28a (+) expression vector. The construct was used to transform Escherichia coli BL21 (DE3). The expression of the protein was induced by the addition of 1mM IPTG. To confirm the expression of the cloned gene, SDS-PAGE was carried out and production of an approximately 11 KD recombinant tagged protein was confirmed for the cloned hbs gene. Results and Conclusion: The identity of the recombinant HBsu was verified and characterized by SDS-PAGE which can then be utilized for further applications. https://ijm.tums.ac.ir/index.php/ijm/article/view/64 https://ijm.tums.ac.ir/index.php/ijm/article/download/64/64