Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 5 2 2013 06 15 113 119 EN Seyed-Mehdi Larrie-Bagha Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. Iraj Rasooli Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. Seyed-Latif Mousavi-Gargari Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. Zohreh Rasooli School of Medicine, Shahed University, Tehran-Iran. Shahram Nazarian Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran-3319118651, Iran. 2015 10 16 Background and Objectives: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 has been recognized as a major food borne pathogen responsible for frequent hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are important reservoirs of E. coli O157:H7, in which the organism colonizes the intestinal tract and is shed in the feces. Objective: Vaccination of cattle has significant potential as a pre-harvest intervention strategy for E. coli O157:H7. The aim of this study was to evaluate active and passive immunization against E. coli O157:H7 using a recombinant protein. Materials and Methods: The recombinant FepA protein induced by IPTG was purified by nickel affinity chromatography. Antibody titre was determined by ELISA in FepA immunized rabbits sera. Sera collected from vaccinated animals were used for bacterial challenge in passive immunization studies. Results: The results demonstrate that passive immunization with serum raised against FepA protects rabbits from subsequent infection. Conclusion: Significant recognition by the antibody of ferric enterobactin binding protein may lead to its application in the restriction of Enterobacteriaceae propagation. https://ijm.tums.ac.ir/index.php/ijm/article/view/626 https://ijm.tums.ac.ir/index.php/ijm/article/download/626/399