Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 18 4 2026 08 04 629 632 Suman Agrawalla Department of Microbiology, College of Basic Sciences and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India Prasanta Mishra Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India Srinivas Sathapathy Department of Veterinary Anatomy and Histology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India Ashok Kumar Sethi Department of Microbiology, College of Basic Sciences and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India Debasis Dash Department of Botany, College of Basic Sciences and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India Archana Mahapatra Department of Veterinary Anatomy, Faculty of Veterinary and Animal Sciences, Banaras Hindu university, Rajiv Gandhi South Campus, Uttar Pradesh, India Radhakrishna Sahu Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India 2025 12 02 2026 06 08 Background and Objectives: Apart from spontaneous deamidation and isomerization of Asp/Asn residues, factors like ageing, oxidative stress, salinity and heat stress may also form abnormal isoaspartate (iso-Asp) residues compromising protein structure and function. PIMT helps in restoration of such iso-Asp residues. Pseudomonas aeruginosa is a gram-negative bacteria responsible for diseases with varied degree of pathogenesis in humans and animals. They can survive a wide range of temperature between 25℃ to 42℃. The objective of the current study was to monitor the expression dynamics of PIMT in Pseudomonas aeruginosa upon exposure to different thermal stresses. Materials and Methods: The mid log phase culture of Pseudomonas aeruginosa was exposed to different temperature (37°C, 42°C, 56°C, 80°C) for an hour. The expression levels of PIMT were quantified by Western blot and ELISA. Results: A relatively higher level of expression of PIMT was observed in the group exposed to 42°C, 56°C, 80°C in comparison to 37°C. The expression of PIMT increased around 1.5 times in the group exposed to 42°C. Conclusion: A temperature dependent overexpression of PIMT was observed in Pseudomonas aeruginosa. https://ijm.tums.ac.ir/index.php/ijm/article/view/5986 https://ijm.tums.ac.ir/index.php/ijm/article/download/5986/1923