Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 18 3 2026 06 07 422 427 Abed Karimpour Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Maral Gharaghani Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran Amir Kamal Hardani Department of Pediatrics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Hadis Jafarian Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Ali Zarei Mahmoudabadi Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2025 07 01 2026 05 05 Background and Objectives: Candida albicans is a common opportunistic pathogen. Genotyping based on the 25S rDNA and mating type locus (MTL) allows for epidemiological and genetic profiling. This study aimed to characterize the genotypes and mating types of C. albicans isolates from various clinical sources in Iran. Materials and Methods: Ninety-four isolates from clinical samples (saliva, urine, vaginal swabs, and skin scrapings) were cultured on CHROMagar Candida and identified by standard phenotypic methods. Genotyping was performed using CA-INT primers, and mating type analysis was conducted using MTLa1 and MTLα1 primers. Results: In this study, 94 isolates of C. albicans from various sources were analyzed. Genotype A was the most frequent (65%), followed by genotypes C (24.5%), B (9.6%), and D (1.1%). Most isolates (97.9%) were heterozygous at the MTL locus, only two isolates homozygous (α/α). Conclusion: Genotype A and MTL-heterozygous strains were predominant among C. albicans isolates, suggesting a consistent molecular pattern across different clinical sources and regions. https://ijm.tums.ac.ir/index.php/ijm/article/view/5596 https://ijm.tums.ac.ir/index.php/ijm/article/download/5596/1902