Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 17 1 2025 02 05 Investigation of the optimal condition for the growth and biofilm development of Candida albicans on three dental materials 153 162 Hoai Le Center of Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam Truong Phuong Faculty of Odonto – Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam Giang Huy Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam Phuoc-Vinh Nguyen Faculty of Pharmacy, University of Health Sciences, Vietnam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam Bac Giang Nguyen Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam 2024 10 03 2024 12 18 Background and Objectives: Candida albicans as pathogenic fungi cause conditions like oral candidiasis and dental caries. The critical role of biofilms in the pathogenicity of C. albicans necessitates the exploration of conditions that promote their growth and development. Our study aimed to delineate the optimal conditions conducive to the proliferation and biofilm production of C. albicans on prevalent dental materials. Materials and Methods: To approximate oral cavity conditions, culture media were enhanced with various glucose concentrations to assess the growth and biofilm-forming capability of the fungus through growth curve analysis and crystal violet assays. Results: The findings suggest that YPG medium augmented with 4% glucose presents as an optimal environment for C. albicans growth. Biofilm formation was most effectively promoted in RPMI medium supplemented with the same concentration of glucose. Composite resin was identified as the substrate most susceptible to biofilm development by C. albicans under these conditions. Conclusion: This investigation highlights the necessity of accounting for microbial activity and material characteristics in the prevention and management of dental biofilm formation. Our research advances the understanding of in vitro cultivation of C. albicans, simulating the oral milieu more accurately and contributing to enhanced oral health management for individuals utilizing temporary dental fixtures. https://ijm.tums.ac.ir/index.php/ijm/article/view/5061 https://ijm.tums.ac.ir/index.php/ijm/article/download/5061/1753