Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 17 3 2025 06 02 404 410 Sara Kamal Shahsavar Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Zahra Esmaeili Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Hamid Ariannejad Department of Animal Sciences, School of Agriculture, Ferdowsi University, Mashhad, Iran Mohammadreza Nassiri Department of Animal Sciences, School of Agriculture, Ferdowsi University, Mashhad, Iran Ali Beheshti Namdar Department of Gastroenterology and Hepatology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Amir Hossein Jafarian Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Ramin Sadeghi Department of Nuclear Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Vahid Reza Dabbagh Department of Nuclear Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Kiarash Ghazvini Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2024 09 13 2024 12 25 Background and Objectives: The growing resistance of Helicobacter pylori to antibiotics poses significant challenges in managing gastric ulcers. The application of IgY antibodies against H. pylori is a promising strategy. The current study evaluated the preventive and synergistic effects of IgY antibodies in conjunction with pantoprazole for treating H. pylori infection. Materials and Methods: This investigation specifically focused on the inhibitory effects of IgY in the AGS cell line infected with H. pylori. In addition, the synergistic activity of IgY with pantoprazole and its preventive and therapeutic effects were assessed in male C57BL/6 mice. Results: The findings indicated that IgY antibodies possess a substantial inhibitory effect on the adhesion of H. pylori to the AGS cell line. Furthermore, IgY antibodies resulted in a significant reduction (P<0.05) in the population of H. pylori and the severity of gastritis in infected C57BL/6 mice in both treatment and prevention groups. Notably, the optimal outcome was observed when IgY was administered alongside pantoprazole. Conclusion: The use of IgY has the potential to repair damaged cells and prevent infection by decreasing bacterial adherence to gastric epithelial cells. Given its synergistic effect with pantoprazole, IgY can be recommended as a suitable complementary treatment in conjunction with pantoprazole. https://ijm.tums.ac.ir/index.php/ijm/article/view/5036 https://ijm.tums.ac.ir/index.php/ijm/article/download/5036/1782