Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 16 5 2024 10 19 584 597 Esraa Al-Nsour Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Isra University, Amman, Jordan Hadeel AL-Hadithi Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Isra University, Amman, Jordan Rania Al-Groom Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Zarqa University, Al-Zarqa, Jordan; Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Zarqa, Jordan Saqr Abushattal Department of Medical Analysis, College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma’an, Jordan Abdallah Naser Department of Applied Pharmaceutical Sciences and Clinical Pharmacy, Faculty of Pharmacy, Isra University, Amman, Jordan Ahmad Al Nsour Department of Otolaryngology (ENT), Jordan University Hospital, Medical Doctor (ENT), Amman, Jordan Rawand Sallam Department of Internal Medicine, Jordan University Hospital, Medical Doctor (Internal Medicine), Amman, Jordan Lara Kollab Department of Internal Medicine, Jordan University Hospital, Medical Doctor (Internal Medicine), Amman, Jordan Laila Alswalha Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Zarqa, Jordan Mohd Sajjad Ahmad Khan Department of Basic Sciences, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia 2024 05 31 2024 09 08 Background and Objectives: Healthcare workers in hospitals are exposed to infectious diseases that occur in the hospital making them a source of infection for the patients. It is interfaced as cross-contamination agents for MRSA and MR-CoNS, and preventive measures need to be adapted accordingly. The study aimed to assess Methicillin-Resistant Staphylococcus (MRS) on the skin and nasal cavities of healthcare workers (HCWs) and identifying isolates to the species level. Materials and Methods: Swab samples were cultured on mannitol salt agar (MSA) to obtain MRS and determine their ability to produce coagulase. Their susceptibility to antibiotics were determined by agar screening and disk diffusion methods and further identification was done at the species level. Results: The highest percentage of methicillin resistant coagulase positive Staphylococci (MRCoPS) was reported among skins of male HCWs, (71.4%) were identified as MRSA. The highest levels of methicillin resistant coagulase negative Staphylococci (MRCoNS) were mainly detected in both nasal cavities, (75%) were identified as MRSE. MRSA was reported from doctors (p-value 0.033), whereas the highest incidence of MRSE was obtained from the nurses (p-value 0.048). Conclusion: This study highlighted that incidence of MRSA was mainly detected in doctors and MRCoNS in both nasal cavities. The highest percentage of MRCoNS was recovered from the patients’ room followed by the reception table. Moreover, vancomycin is suggested to be highly effective in managing and controlling S. aureus, MRSA- and MRSE related infections. https://ijm.tums.ac.ir/index.php/ijm/article/view/4810 https://ijm.tums.ac.ir/index.php/ijm/article/download/4810/1704