Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 16 6 2024 12 08 780 785 Emad Asgari Department of Theriogenology, School of Veterinary Medicine, University of Tehran, Tehran, Iran Zohreh Sharifi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Farahnaz Bineshian Department of Parasitology and Mycology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Mahsa Zamanian Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 2024 04 14 2024 12 07 Background and Objectives: Recently, the anti-herpetic activities of different plant species have been investigated. This study evaluated the effects of Teucrium stocksianum aqueous extract on the HSV-1 virus-infected Vero cell. Materials and Methods: The IC50 of the aqueous extract was obtained by the maceration of the plant in boiling water and has been measured with the MTT method, also the q-PCR was used to study viral gene expression reduction. Results: Results of the MTT test indicated that the highest percentage of metabolic activity was observed in the 75 μg/ml concentration of Teucrium stocksianum’s aqueous extracts (IC50=45.5μg/ml). Time intervals of 24 and 48 hours after viral infection revealed that the cell viability is reduced by the viral infection time (MOI=0.1), log 10-3, p <0.001). Furthermore, the plant’s aqueous extract concentration almost avoids cell viability reduction. Through Q-PCR results; the reduction of viral proliferation revealed that the low expression of genes UL46 and US6 were significant in the presence of different treatments utilized in the experiment. Conclusion: T. stocksianum, has an anti-viral property and may be considered as a remedy for anti-HSV-1 agents. https://ijm.tums.ac.ir/index.php/ijm/article/view/4723 https://ijm.tums.ac.ir/index.php/ijm/article/download/4723/1729