Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 16 3 2024 06 19 351 356 Rajab Mardani Department of Viral Vaccines, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran Ariana Alavi Department of Production, Bsc in Biotechnology Pharmaceutical and Drug Delivery System Labratoary, Pasteur Institute of Iran, Tehren, Iran Seyed Dawood Mousavi Nasab Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran, Tehran, Iran Nayebali Ahmadi Proteomics Research Center, Department of Medical Lab Technology, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Javad Hossein Tehrani Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Maryam Shahali Department of Viral Vaccines, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran Delaram Doroud Department of Production, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran 2024 02 21 2024 05 05 Background and Objectives: Lipoarabinomannan is one of the components of the significant structural cell surfaces of mycobacteria and serves as an immunostimulatory factor. TNF-α and IL-12 are two examples of the anti-bacterial inflammatory cytokines that are activated and induced during infection. Materials and Methods: In this study, mannan was extracted and processed, and then Bulb/c female mice were used in three groups, one group was given BCG vaccine, the other group was given BCG vaccine with mannan adjuvant, and a non-injected group was used as a control group. Inflammatory factors interleukin-12, TNF-α, IgG and IgM were measured in mouse serum. Results: The levels of the inflammatory factors interleukin-12 and TNF-α in the serum isolated from mice receiving the BCG vaccine with mannan adjuvant showed a significant difference compared to the group that received only the BCG vaccine and the control group [IL-12] and , with P≤0.05.The examination of the level of IgG immune factors in these three groups revealed a significant difference. The group that received the BCG vaccine with mannan adjuvant showed a marked contrast compared to the group that received only the BCG vaccine and the control group, with P≤0.05. The level of IgM was higher in the group that received the BCG vaccine alone compared to the adjuvant vaccine group and the control group, with P≤0.05. Conclusion: Our results indicated that mice receiving the BCG vaccine with mannan adjuvant had significantly higher serum levels of IL-12, TNF-α, and IgG than the group receiving BCG alone. https://ijm.tums.ac.ir/index.php/ijm/article/view/4620 https://ijm.tums.ac.ir/index.php/ijm/article/download/4620/1676