Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 16 3 2024 06 19 421 427 EN Rahim Soleimani-Jelodar Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Arash Arashkia Department of Virology, Pasteur Institute of Iran, Tehran, Iran Zabihollah Shoja Department of Virology, Pasteur Institute of Iran, Tehran, Iran Kimia Sharifian Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Setareh Akhavan Department of Gynecology Oncology, Imam Khomeini Hospital Complex, Valiasr Hospital Tehran, Iran Fariba Yarandi Department of Obstetrics and Gynecology, Yas Hospital, Tehran, Iran Somayeh Jalilvand Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2024 01 14 2024 04 11 Background and Objectives: Although several studies have been achieved on the frequency of the HPV types among women with cervical cancer in Iran, HPV-positive samples were in some cases directed to specific-primer genotyping of HPV 16 and 18. Therefore, the other HPV types are underestimated. Several studies have also reported a greater prevalence of HPV 16 in cervical cancer in Iran than in the world. To clarify these subjects, the distribution of HPV types in women referred for colposcopy in Tehran was investigated. Materials and Methods: In this cross-sectional study, a total of 148 cervical samples from women with normal, atypical squamous cells of undetermined significance, cervical intraepithelial neoplasia I-III, and invasive cervical cancer histopathology were included. HPV was detected by PCR assay and all HPV-positive specimens were subjected to direct nucleotide sequencing. Results: Our results demonstrated that the total prevalence of HPV was 92.5%. The five most common HPV types were HPV 16 (49.3%), 18 (14.8%), 6 (7.4%), 31 (4.1%), and 11 (2.7%). About the histopathological stage, HPV 16 and 18 were dominant in all studied groups. In cervical cancer, HPV 16 and 18 were detected in 60% and 20% of cases, respectively. Conclusion: HPV 16 and 18 were the most common in cervical cancer in Iran. https://ijm.tums.ac.ir/index.php/ijm/article/view/4569 https://ijm.tums.ac.ir/index.php/ijm/article/download/4569/1684