Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 15 6 2023 11 20 771 778 Hind Bennani Laboratory of Microbiology, Mohamed VI University Hospital Center, Marrakesh, Morocco; Laboratory of Microbiology, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakesh, Morocco Morad Guennouni Department of Science and Technology Team, Higher School of Education and Training, Chouaîb Doukkali University, El Jadida, Morocco; Laboratory of Health Sciences and Technologies, Higher Institute of Health Sciences of Settat, Hassan First University of Settat, Settat, Morocco Assia Ouarradi Laboratory of Microbiology, Mohamed VI University Hospital Center, Marrakesh, Morocco; Laboratory of Microbiology, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakesh, Morocco Asmae Lamrani Hanchi Laboratory of Microbiology, Mohamed VI University Hospital Center, Marrakesh, Morocco; Laboratory of Microbiology, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakesh, Morocco Nabila Soraa Laboratory of Microbiology, Mohamed VI University Hospital Center, Marrakesh, Morocco; Laboratory of Microbiology, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakesh, Morocco 2023 02 09 2023 10 14 Background and Objectives: A new type of corona virus has caused Corona virus disease-19 and, subsequently, a global pandemic. All individuals are prone to the disease, so drastic measures were taken to prevent its spread. This study aimed to evaluate the impact of COVID-19 on the progression of the antimicrobial resistance rate by comparing two periods: before and during COVID-19. Materials and Methods: We used a cross-sectional design to investigate the Antimicrobial Resistance (AMR) rate before (03/2019 to 03/2020) and during COVID-19 (03/2020 to 03/2021) in a University Hospital in Marrakech. The data were analyzed using SPSS Version 25.0. Results: Among the 7106 specimens, there was a significant increase in the multidrug-resistant bacterial from 27.38% to 35.87% during COVID-19 (p<0.001), particularly in blood culture, cerebrospinal fluid, catheter, and pus. However, there was a non-significant change in puncture fluid, expectoration, protected distal sampling, joint fluid, stool culture, and genital sampling. A decrease in Multidrug-resistant bacteria (MDRB) was observed only in cytobacteriological urine tests (p<0.05). According to species, there was an increase in extended-spectrum beta-lactamase-producing Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae, and methicillin-resistant Staphylococcus aureus. Conclusion: In our study, it is particularly noticeable that the MDRB has increased. These results highlight the importance that the pandemic has not been able to slow the progression. https://ijm.tums.ac.ir/index.php/ijm/article/view/4122 https://ijm.tums.ac.ir/index.php/ijm/article/download/4122/1624