Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 15 2 2023 04 19 311 317 Safari Jatmiko Department of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia; Department of Immunology and Molecular Biology, Faculty of Medicine, Universitas Muhammadiyah Surakarta, Surakarta, Indonesia Hartono Hartono Department of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia; Department of Physiology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia Tonang Ardyanto Department of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia Dono Indarto Department of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia; Department of Physiology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia 2022 12 30 2023 02 25 Background and Objectives: Intestinal leakage commonly occurs in severe dengue infection with zonulin as a biomarker. The aim of this study was to determine the effects of NS1 on liver weight, zonulin expression and serum zonulin levels. Materials and Methods: This laboratory experiment used 18 ddY mice, which were randomly divided into control (C), PBS (T1), and PBS + NS1 (T2) groups. Mice in the T1 and T2 groups were intravenously injected with 500 µl PBS only and 50 µg NS1 respectively. Mice blood samples were collected before and after three-day treatment for measurement of zonulin level. The fresh liver was weighted directly and were then used for immunostaining. Results: The C group had lower wet liver weight compared to the T groups (p=0.001). Increased expression of liver zonulin was found in the T2 group, significant different from the C (p=0.014) and T1 groups (p=0.020). After treatment, serum zonulin levels in the T1 group was higher than that of the T1 group before treatment (p=0.035) but not in control (p=0.753) and T2 groups (p=0.869). Conclusion: Administration of 50 µg NS 1 increases wet liver weight and zonulin expression in hepatocytes, but did not increase serum zonulin levels in ddY mice. https://ijm.tums.ac.ir/index.php/ijm/article/view/4061 https://ijm.tums.ac.ir/index.php/ijm/article/download/4061/1571