Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 6 4 2014 08 15 246 252 EN Hossein Khoshkharam-Roodmajani Department of Bacteriology and Virology, Faculty of Medicine AND Student Research Committee. Jamal Sarvari Department of Bacteriology and Virology, Faculty of Medicine. Abdollah Bazargani Department of Bacteriology and Virology, Faculty of Medicine. Mohammad Reza Kandekar-Ghahraman Department of Bacteriology and Virology, Faculty of Medicine. Ali Nazari-Alam Department of Bacteriology and Virology, Faculty of Medicine. Mohammad Motamedifar Department of Bacteriology and Virology, Faculty of Medicine AND Shiraz HIV/AIDS Research Center (SHARC), Shiraz University of Medical Sciences, Shiraz, Iran. 2015 10 16 Background and Objectives: To investigate coagulase gene polymorphisms of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. Materials and Methods: A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP. Results: There were 27 and 28 different RFLP patterns for AluI and HaeIII restriction enzymes respectively. This study showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme. Conclusion: PCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates. https://ijm.tums.ac.ir/index.php/ijm/article/view/397 https://ijm.tums.ac.ir/index.php/ijm/article/download/397/174