Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 15 1 2023 02 15 62 68 Sara Sadeqi Department of Medical Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran Slawomir Michniewski Department of Life Sciences, University of Warwick, Coventry, United Kingdom Farhad Nikkhahi Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Eleanor Jameson Department of Life Sciences, University of Warwick, Coventry, United Kingdom AND Department of Natural Sciences, Bangor University, Bangor, Gwynedd, United Kingdom Seyed Mahmoud Amin Marashi Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran 2022 09 11 2023 01 11 Background and Objectives: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections. Materials and Methods: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology. Results: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1. Conclusion: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections. https://ijm.tums.ac.ir/index.php/ijm/article/view/3899 https://ijm.tums.ac.ir/index.php/ijm/article/download/3899/1543