Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 6 5 2014 10 15 311 316 EN Hadis Amraie Department of Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran. Pegah Shakib Cellular & Molecular Research Center and Microbiology Department, Member of Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran. Samaneh Rouhi Cellular & Molecular Research Center and Microbiology Department, Member of Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran. Neda Bakhshandeh Applied Agriculture Branch, Semnan Applied Sciences and Technology University, Semnan, Iran. Behnam Zamanzad Department of Microbiology, Shahrekord University of Medical Sciences, ShahreKord, Iran. 2015 10 16 Background and Objectives: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic microorganism. This study aimed to investigate the presence of magA gene and antimicrobial susceptibility in K. pneumoniae. Materials and Methods: 195 clinical specimens were collected from hospitals of Shahrekord, Iran. Bacterial culture, biochemical diagnostic standard test, determination of antibiotic sensitivity, phenotypic testing hypermucoviscosity (HV) and polymerase chain reaction (PCR) was performed for isolation and characterization of K. pneumoniae. Results: 173 samples were positive for K. pneumoniae. The highest and lowest rates of resistance were related to amoxicil-lin 79.19% and ciprofloxacin 15.60%, respectively. Also 4 samples were positive for magA gene. Conclusion: Based on our results, K. pneumoniae strains were resistant to different antibiotics. Knowing how to identify strains of K. pneumoniae, spreading of its virulence and also antimicrobial resistance genes can be useful in treatment of infection caused by this bacterium. https://ijm.tums.ac.ir/index.php/ijm/article/view/379 https://ijm.tums.ac.ir/index.php/ijm/article/download/379/156