Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 14 6 2022 12 06 841 849 Ali Ehsan Karshenas Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran Taghi Zahraei Salehi Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran Maryam Adabi Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran Babak Asghari Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Ramak Yahyaraeyat Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 2022 06 05 2022 10 27 Background and Objectives: Antibiotics-resistant Escherichia coli strains are considered one of the most important causes of human and animal infections worldwide. The aim of current study was to detect common resistance (carbapenems and quinolones) genes by PCR. Materials and Methods: A total of 100 E. coli strains isolated from human urinary tract infection and 20 isolated strains of aborted sheep embryos were collected. PCR was performed using specific primers to detect the resistance genes. Results: Overall, among the quinolones resistance genes, qnrS resistance gene had the highest frequency (48%) and among carbapenem resistance genes, imp resistance gene had the highest frequency (45%). The frequency of resistance genes, IMP (28.45%), KPC (9.5%), VIM (9.15%), NDM (7.20%) were observed in clinical and veterinary strains, respectively. According to the results, 38.6% of E. coli strains had at least one from five genes of resistance to quinolones. The lowest frequency of resistance gene was related to qnrA, which was observed in only 29 (24.2%) strains. Conclusion: Monitoring of carbapenem and quinolone resistance in pathogenic E. coli to humans and animals has an important value in revising treatment guidelines and the national public health, and plays an important role in preventing the spread of resistant strains. https://ijm.tums.ac.ir/index.php/ijm/article/view/3757 https://ijm.tums.ac.ir/index.php/ijm/article/download/3757/1525