Tehran University of Medical Sciences Iranian Journal of Microbiology 2008-3289 14 4 2022 08 14 568 573 Mohsen Keshavarz The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran Nahid Janati-Namin Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Yaser Arjeini Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran Talat Mokhtari-Azad Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Farhad Rezaei Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2022 04 29 2022 05 18 Background and Objectives: Parvovirus B19 (B19V) is usually transmitted through respiratory tract, but can also be received through blood transfusion. This study evaluated the seroprevalence, DNA existence, and circulating genotypes of B19V in hemophilia patients. Materials and Methods: Serum samples of cases and controls were analyzed for B19V using ELISA and real-time PCR. Finally, obtained sequences were used for genotyping. Results: Among cases, 3% were anti-B19V IgM positive and 47% were anti-B19V IgG positive and B19V DNA was detected in 16% of them. However, among controls, 38% were anti-B19V IgG positive (P>0.05) and 5% were B19V DNA positive (P= 0.019). Also ~13% of cases were positive and all of controls were negative for IgG avidity test (P= 0.029). Viral load in case group was higher than control group (P = 0.037). Conclusion: Since hemophilia patients receive large amounts of blood factors, prevalence of B19V in these patients might be higher than normal subjects. https://ijm.tums.ac.ir/index.php/ijm/article/view/3688 https://ijm.tums.ac.ir/index.php/ijm/article/download/3688/1492